A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-9427R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000434783
Collection NIG_RNAi_Fly-1   Associated gene(s) CG9427
Genomic Location
Chromosome (arm) 3R Sequence location 3R:5,450,947..5,451,511 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0082075 FBtr0334616 FBtr0334617 FBtr0082076 FBtr0082104 FBtr0334619 FBtr0082077 FBsf0000015891 FBsf0000026416 FBsf0000015975 FBsf0000195023 FBsf0000081236 FBsf0000092620 FBsf0000413009 FBsf0000405894 FBsf0000408242 FBsf0000395475 FBsf0000395555
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 370
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
GGCTGAGTGCAAGGGCAATCGCCTCCAGAGTTGCGTCATCAACAGCACCG
CAAACCAGGCGGCGCAGGTTAAATTCGTGGTGTGCCAGATGCTAGCTCCG
GATTATTCCCGCATTGATCAGTGTGCCAATGAGGcAGGCCTGCTCACCGA
TGTGGTCCACTGCCTGTCCAGCGAGACGGGCACCAAATTGCAGCTGCAGG
CGGAGCTGGNTGACCAAACAGTACAGCCCGAGCTTCATTCCCACCATCGT
CTACAATGGCGTCTTCGATCAGCAGCTGCAGGANCCATTCGCTGCGcGAT
TTCCGCGGCACGGTTTGTTATATGTTGCGTCAACAAAANCTTGCTGCCCA
GCAGCAGCACAATCTGCCAG
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgGGCTGAGTGCAAGGGCAATCG
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccCTGGCAGATTGTGCTGCTGCT
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
9427R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-9427R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]