A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-9503R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000434808
Collection NIG_RNAi_Fly-1   Associated gene(s) CG9503
Genomic Location
Chromosome (arm) X Sequence location X:14,815,001..14,815,611 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0073952 FBtr0073950 FBtr0330215 FBtr0073951 FBtr0073949 FBtr0330217 FBtr0330216 FBtr0330214 FBtr0073974 FBsf0000020523 FBsf0000019575 FBsf0000073866 FBsf0000405508 FBsf0000398269
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Length 504
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
CGGCCAGTTTGGAGGAGCGGTAACGGCGGCCACCAGCAACAGTTGGTTCA
TCCCGATGCTGATGGCAGCGGTGGCCTATTTTCAGTACGAGGAGATCATC
GATCCGGAATCAAAGCCCAGTGATGTGGGCGGCGACGACATCCTCGACCA
TTATGACTTTATAGTGATAGGTGCCGGATCGGCGGGGGCCGTGNGTGGCG
AATCGATTGACGGAGGTGGAGAACTGGAATGTACTTTTGCTGGAGGCCGG
TGGCGATGAAACGGAACTCACGGACGTCCCTTTGATGGCCGGCTACCTGC
AGCTGTCCAAGATTGACTGGCAGTACAAGACGGAACCATCGGGAACATCT
TGCTTGGCAATGCAAGGAGGTCGCTGCAATTGGCCCAGAGGCAAGGTTCT
TGGCGGCAGCTCTGTTCTTAACTATATGTTGTATCTGCGAGGATCTAAGC
ATGACTACGACAACTGGGAGGCTATGGGCAATCCCAGTTGGTCGTATCNG
CGAC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgCGGCCATTGGAGGAGCGGTAA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccGTCGCGATACGACCAACTGGG
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
9503R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-9503R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]