A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-9668R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000434881
Collection NIG_RNAi_Fly-1   Associated gene(s) Rh4
Genomic Location
Chromosome (arm) 3L Sequence location 3L:16,846,274..16,855,415 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0075330 FBtr0075332 FBtr0075333 FBtr0301683 FBtr0075336 FBtr0332702 FBtr0075338 FBtr0075337 FBsf0000011017 FBsf0000009544 FBsf0000020807 FBsf0000009543 FBsf0000010889 FBsf0000024958 FBsf0000010729 FBsf0000071457 FBsf0000072231 FBsf0000091004 FBsf0000077160 FBsf0000092956 FBsf0000074075 FBsf0000089557 FBsf0000082975 FBsf0000081625 FBsf0000194213 FBsf0000082020 FBsf0000437430 FBsf0000405927 FBsf0000407812 FBsf0000413074 FBsf0000408751 FBsf0000403132 FBsf0000398879 FBsf0000437921 FBsf0000390821 FBsf0000392106 FBsf0000391950 FBsf0000429652
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 500
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
CATGCACTACATGCTGGGCGTCTTCTACATATTTCTCTTCTGCGCCTCGA
CAGTGGGTAATGGCATGGTCATCTGGATCTTCAGCACATCCAAGTCGCTG
AGGACACCATCCAATATGTTCGTGCTGAATCTGGCCGTCTTCGATCTGAT
CATGTGCCTCAAGGCGCCGATCTTCATCTACAACAGCTTCCATCGAGGAT
TCGCCCTGGGCAATACCTGGTGCCAGATATTCGCCTCCATTGGCTCCTAT
TCGGGTATTGGTGCTGGTATGACGAACGCGGCCATAGGATACGATCGATA
CAATGTGATCACCAAGCCCATGAACCGCAACATGACCTTCACCAAGGCGG
TGATAATGAATATAATCATCTGGTTGTACTGCACACCGTGGGTTGTCCTG
CCGCTAACCCAGTTCTGGGATCGATTCGTGCCAGAGGGCTACCTCACGTC
CTGCTCCTTCGACTATCTTTCGGACAACTTTGACACCCGGTTGTTTGTGG
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgCATGCACTACATGCTGGGCGT
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccCCACAAACAACCGGGTGTCAA
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
9668R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-9668R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]