FB2025_01 , released February 20, 2025
Cell Line: S3
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General Information
Symbol
S3
Species
D. melanogaster
Feature type
cultured cell-line
FlyBase ID
FBtc0000005
Source
Oregon R
(Cherbas, 2008.11.12)
Tissue Source
embryo
(Schneider, 1972)
Developmental Stage
late embryonic stage
(Cherbas, 2008.11.12)
Lab of Origin

Schneider

(Schneider, 1972)
Characterization
Sex
male (based on expression levels of roX genes, msl-2 and traF; splicing patterns of tra and Sxl)
(Stoiber et al., 2016, Lee et al., 2014)
Karyotype
Associated Genes (0)
    Integrated Constructs (0)
      Comments

      New stable cell line derived from this cell line: S3[GCaMP3] cells were created that contain the GCaMP3 calcium sensor inserted in the GCaMP3 pAc5.1/V5-His A vector (Invitrogen).

      (Sung et al., 2017)

      Expression profiling by genome tiling array for this cell line (response to 5 hr ecdysone treatment) may be found at GEO: GSE16269 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11280).

      (Stoiber et al., 2016)

      Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16288 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16288).

      (Cherbas et al., 2011)

      S3 cells were made from Oregon R embryos on the verge of hatching.

      (Cherbas, 2008.11.12)

      Adherent, spindle-shaped cells, ecdysone-responsive, grow in clumps.

      (Ramadan et al., 2007)
      Parental Lines (0)
      Descendant Lines (0)
       
      External Stocks and Resources ( 3 )
      Crossreferences
      Cellosaurus - Cellosaurus is a knowledge resource on cell lines that attempts to describe all cell lines used in biomedical research.
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      RRID - Research Resource Identification Portal
      Synonyms and Secondary IDs (9)
      References (66)