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General Information
Symbol
S2R+
Species
D. melanogaster
Feature type
FlyBase ID
FBtc0000150
Source
Tissue Source
Lab of Origin
Schneider
Characterization
Sex
male (based on expression levels of roX genes, msl-2 and traF; splicing patterns of tra and Sxl)
Karyotype
Integrated Constructs (0)
    Comments
    A knockout gig S2R+ cell line was used.
    Both S2R+ and S2R+Wb (Wolbachia) cells were used.
    Stable cell lines generated:Stable cell lines containing C-terminal and N-terminal fusions of VAP and VAP(P58S) with GFP were created.
    Flag-HA-Ago2 stable S2R+ cells from the laboratory of Eric Lai were utilized.
    S2R+ cells stably expressing Actin-GAL4 (obtained from the laboratory of Satyajit Mayor, NCBS, Bangalore) were utilized.
    S2R+ cells stably expressing Actin-GAL4 were used.
    Stable cell line generated: S2R+ cells were stably transfected with a pCasper-tub-mitoGFP encoding a tubulin promoter driven human COX VIII mitochondrial targeting signal fused to the N-terminus of EGFP.
    Stable cell line generated: Stable cell lines were generated to express constructs containing destabilizing domains (genetic tags that conditionally control the level of abundance of proteins of interest with specific stabilizing small-molecule drugs).
    Wild type, TSC1 knockout (TSC1KO) and TSC2 (TSC2KO) knockout S2R+ cells were used.
    Stable cell line generated: a stable S2R+ cell line expressing AkhR was generated.
    Expression of general hemocyte markers observed.
    Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16287 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16287).
    S2R+ is an isolate of S2 that has receptors for wg signalling. It is more strongly adherent to the substrate than other S2 cells available from the DGRC.
    SR2+ is an isolate of S2 cells that was found in the Miyake laboratory freezer. It was contributed to the Miyake lab by Imogene Schneider and is likely to be very similar to the original S2 line.
    Hemocyte-like gene expression, phagocytic, adherent, flat cells; Fz+ and Wg-responsive.
    S2R+ cells express fz and fz2, and respond to the addition of extracellular wg protein by elevating arm protein levels and hyperphosphorylating dsh protein.
    Parental Lines (1)
    Isolate of:
    Descendant Lines (11)
    External Stocks and Resources ( 1 )
    Crossreferences
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (14)
    Reported As
    Symbol Synonym
    CVCL_Z831
    RRID:CVCL_Z831
    S2 receptor plus
    S2R+
    (Chaplot et al., 2019, Domsch et al., 2019, Drees et al., 2019, Fattouh et al., 2019, Gil-Ranedo et al., 2019, Gultekin and Steller, 2019, Guo et al., 2019, Kim and Choi, 2019, Kreutzberger et al., 2019, Li et al., 2019, Mathre et al., 2019, Meschi et al., 2019, Moreno et al., 2019, O'Connell et al., 2019, Palazzo et al., 2019, Petri et al., 2019, Rass et al., 2019, Sharma et al., 2019, Skouloudaki et al., 2019, Valoskova et al., 2019, Vijayakumar et al., 2019, von Stockum et al., 2019, Wang et al., 2019, Won et al., 2019, Xu et al., 2019, Zirin et al., 2019, Allen and Seo, 2018, Anding et al., 2018, Baena-Lopez et al., 2018, Bakthavachalu et al., 2018, Balakrishnan et al., 2018, Basso et al., 2018, Becker et al., 2018, Billmann et al., 2018, Brown et al., 2018, Courgeon et al., 2018, Cusumano et al., 2018, Duan et al., 2018, Giordano et al., 2018, Ji et al., 2018, Kistler et al., 2018, Knuckles et al., 2018, Kogenaru and Isalan, 2018, Kullmann and Krahn, 2018, Li et al., 2018, Lin et al., 2018, Lin et al., 2018, Lin et al., 2018, Lloyd et al., 2018, Ma et al., 2018, Mallik et al., 2018, Manieu et al., 2018, Mazzotta et al., 2018, Mohr et al., 2018, Panda et al., 2018, Poon et al., 2018, Reichardt et al., 2018, Ripp et al., 2018, Rosin et al., 2018, Szabo et al., 2018, Tacchelly-Benites et al., 2018, Tang et al., 2018, Wang et al., 2018, Wang et al., 2018, Yatsenko and Shcherbata, 2018, Zouaz et al., 2018, Amarnath et al., 2017, Arenas et al., 2017, Cherbas et al., 2017.4.19, Epstein et al., 2017, Ewen-Campen et al., 2017, Housden et al., 2017, Jeong et al., 2017, Kamalesh et al., 2017, Li et al., 2017, Lin et al., 2017, Lin et al., 2017, Lippert et al., 2017, Liu et al., 2017, Mallik et al., 2017, Moraru et al., 2017, Paik et al., 2017, Palazzo et al., 2017, Perez-Mockus et al., 2017, Pirone et al., 2017, Sanfilippo et al., 2017, Song et al., 2017, Spannl et al., 2017, Yeh et al., 2017, Zheng et al., 2017, Ammeux et al., 2016, Bairoch, 2016, Bertolin et al., 2016, Boone et al., 2016, Brinkmann et al., 2016, Flegel et al., 2016, Girdler et al., 2016, Guo et al., 2016, Lence et al., 2016, Miettinen and Björklund, 2016, Sen and Cox, 2016, Senaratne et al., 2016, Sen et al., 2016, Truscott et al., 2016, Vinayagam et al., 2016, Wang et al., 2016, Wang et al., 2016, Wang et al., 2016, Wang et al., 2016, Zhang et al., 2016, Gaspar et al., 2015, González-Morales et al., 2015, Hebbar et al., 2015, Kumichel et al., 2015, Mabashi-Asazuma et al., 2015, Parchure et al., 2015, Ramírez et al., 2015, Stoiber et al., 2015, Verboon et al., 2015, Wu et al., 2015, Zobel et al., 2015, Hwang et al., 2014, Lee et al., 2014, Qing et al., 2014, Manning et al., 2013, Colombani et al., 2012, Lloyd et al., 2012, Cherbas et al., 2011, Lachkar et al., 2010, Lee et al., 2009, Maurer et al., 2009, Nahvi et al., 2009, Belenkaya et al., 2008, Cherbas, 2008.11.12, Kaplan et al., 2008, Leibfried et al., 2008, Rencus-Lazar et al., 2008, Wu et al., 2008, Bilic et al., 2007, Franklin-Dumont et al., 2007, Kim et al., 2007, Kondylis et al., 2007, Peretz et al., 2007, Ramadan et al., 2007, Ayoob et al., 2006, Bartscherer et al., 2006, Ma et al., 2006, Rives et al., 2006, Yang and Bashaw, 2006, Gesellchen et al., 2005, Heeger et al., 2005, Sommer et al., 2005, Baeg et al., 2004, Bogdan et al., 2004, Yanagawa et al., 1998)
    Schneider's 2
    Schneider's line 2
    Name Synonyms
    Secondary FlyBase IDs
      References (510)