Cell Line: S2R+
Cell Line: S2R+
Stable cell line generated: Stable cell lines containing separase cleavage reporter constructs consisting of the MtnA promoter driving expression of cid for centromere localization fused to RFP and EGFP separated by CG8712 or vtd wild-type and mutant separase cleavage sites.
Stable cell lines generated:expressing RNAi resistant versions of RN-tre tagged with Tag:Myc under the control of a MT promoter.
An S2R+ cell line with a knockout gig mutation was used.
Both S2R+ and S2R+Wb (Wolbachia) cells were used.
S2R+ cells were obtained from the laboratory of N. Yanagawa.
Stable cell lines generated: Stable S2R+ cell lines containing C-terminal and N-terminal fusions of Vap33 or Vap33(P58S) with GFP were created.
Tag:FLAG-Tag:HA-AGO2 stable S2R+ cells from the laboratory of Eric Lai were used.
S2R+ cells stably expressing Actin-GAL4 (obtained from the laboratory of Satyajit Mayor, NCBS, Bangalore) were used.
S2R+ cells stably expressing Actin-GAL4 were used.
Stable cell line generated: S2R+ cells were stably transfected with a pCasper-tub-mitoGFP encoding a tubulin promoter driven human COX VIII mitochondrial targeting signal fused to the N-terminus of EGFP.
Stable cell line generated: Stable cell lines were generated to express constructs containing destabilizing domains (genetic tags that conditionally control the level of abundance of proteins of interest with specific stabilizing small-molecule drugs).
Stable cell line generated: a stable S2R+ cell line expressing AkhR was generated.
Expression of general hemocyte markers observed.
Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16287 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16287).
S2R+ is an isolate of S2 that has receptors for wg signalling. It is more strongly adherent to the substrate than other S2 cells available from the DGRC.
SR2+ is an isolate of S2 cells that was found in the Miyake laboratory freezer. It was contributed to the Miyake lab by Imogene Schneider and is likely to be very similar to the original S2 line.
Hemocyte-like gene expression, phagocytic, adherent, flat cells; Fz+ and Wg-responsive.
S2R+ cells express fz and fz2, and respond to the addition of extracellular wg protein by elevating arm protein levels and hyperphosphorylating dsh protein.