Schneider
Both S2R+ and S2R+Wb (Wolbachia) cells were used.
S2R+ cells were obtained from the laboratory of N. Yanagawa.
Stable cell line generated: Stable cell lines were generated to express constructs containing destabilizing domains (genetic tags that conditionally control the level of abundance of proteins of interest with specific stabilizing small-molecule drugs).
Stable cell line generated: a stable S2R+ cell line expressing AkhR was generated.
Expression of general hemocyte markers observed.
Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16287 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16287).
SR2+ is an isolate of S2 cells that was found in the Miyake laboratory freezer. It was contributed to the Miyake lab by Imogene Schneider and is likely to be very similar to the original S2 line.
Hemocyte-like gene expression, phagocytic, adherent, flat cells; Fz+ and Wg-responsive.