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General Information
Symbol
S2-DRSC
Species
D. melanogaster
Feature type
FlyBase ID
FBtc0000181
Source
Tissue Source
Lab of Origin

Schneider

Characterization
Sex
male (based on expression levels of roX genes, msl-2 and traF; splicing patterns of tra and Sxl)
Karyotype
Integrated Constructs (0)
    Comments

    Stable cell line generated:Stable cell line generated:Stable S2 cell lines were generated expressing l(2)gl constructs.

    Stable cell lines generated:An S2 cell line co-expressing Lgl3A-RFP and Lgl-GFP and an S2 cell line expressing Lgl-Dendra2 were created.

    Stock #181 from DGRC

    Expression profiling by genome tiling array for this cell line (response to 5 hr ecdysone treatment) may be found at GEO: GSE16269 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11280).

    Expression of cell-type specific markers correlates with that of plasmocytes and crystal cells; likely that this lines contains a mixture of cell types.

    Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16286 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16286).

    RNA-Seq transcription profile for this cell line may be found at GEO: GSE15596 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE15596). See also the FlyBase collection report modENCODE_mRNA-Seq_cell.A (FBlc0000116) and FlyBase RNA-Seq GBrowse view.

    S2-DRSC is one of the four lines chosen for extensive analysis by the modENCODE project.

    This S2 isolate is the one routinely used for RNAi screens at the DRSC; it is referred to at the DRSC as "S2". Its relationship to the S2-DGRC line is not known.

    Parental Lines (1)
    Isolate of:
    Descendant Lines (0)
     
    External Stocks and Resources ( 1 )
    Crossreferences
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (10)
    References (124)