FB2025_04 , released October 2, 2025
Cell Line: S2-DRSC
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General Information
Symbol
S2-DRSC
Species
D. melanogaster
Feature type
cultured cell-line
FlyBase ID
FBtc0000181
Source
Oregon R
(Cherbas, 2008.11.12)
Tissue Source
embryo
(Schneider, 1972)
Developmental Stage
late embryonic stage
(Cherbas, 2008.11.12)
Lab of Origin

Schneider

(Schneider, 1972)
Characterization
Sex
male (based on expression levels of roX genes, msl-2 and traF; splicing patterns of tra and Sxl)
(Stoiber et al., 2016, Lee et al., 2014)
Karyotype
Associated Genes (0)
    Integrated Constructs (0)
      Comments

      New stable cell line derived from this cell line: A stable Atat knockout S2 cell line was created

      (Ventura Santos et al., 2023)

      New stable cell line derived from this cell line: S2 cells expressing Tag:FLAG,Tag:HA-tagged not under the control of the MT promoter.

      (Erez et al., 2021)

      New stable cell line derived from this cell line: A stable S2 cell line was created containing a FBto0000031:GFP reporter under the control of the Mmp1 promoter.

      (Geigges et al., 2020)

      New stable cell line derived from this cell line: A stable S2 cell line co-expressing l(2)gl-RFP(Unk) and l(2)gl-GFP was created. A stable S2 cell line expressing l(2)gl-Dendra2 was created. A stable S2 cell line expressing l(2)gl-GFP and mCherry-tubulin (FBrf0227229) was used.

      (Moreira et al., 2019)

      New stable cell line derived from this cell line: An S2 cell line stably co-expressing l(2)gl.3A-RFP(Unk) and l(2)gl-GFP and an S2 cell line stably expressing l(2)gl-Dendra2 were created.

      (Moreira et al., 2019)

      Stock #181 from DGRC

      (Panhale et al., 2019)

      New stable cell line derived from this cell line: Stable S2 cell lines were created that express mir-8.

      (Umegawachi et al., 2017)

      Expression profiling by genome tiling array for this cell line (response to 5 hr ecdysone treatment) may be found at GEO: GSE16269 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11280).

      (Stoiber et al., 2016)

      Expression of cell-type specific markers correlates with that of plasmocytes and crystal cells; likely that this lines contains a mixture of cell types.

      (Cherbas et al., 2011)

      Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16286 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16286).

      (Cherbas et al., 2011)

      RNA-Seq transcription profile for this cell line may be found at GEO: GSE15596 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE15596). See also the FlyBase collection report modENCODE_mRNA-Seq_cell.A (FBlc0000116) and FlyBase RNA-Seq GBrowse view.

      (Cherbas et al., 2011)

      New stable cell line derived from this cell line: S2 cells stably expressing an inducible construct with the signal sequence of Drosophila Bip appended to Arus\HRP were used to monitor secretion.

      (von Blume et al., 2009)

      S2-DRSC is one of the four lines chosen for extensive analysis by the modENCODE project.

      (Cherbas, 2008.11.12)

      This S2 isolate is the one routinely used for RNAi screens at the DRSC; it is referred to at the DRSC as "S2". Its relationship to the S2-DGRC line is not known.

      (Cherbas, 2008.11.12)

      New stable cell line derived from this cell line: S2 cells stably expressing the signal sequence of Drosophila Bip appended to Arus\HRP and a Tag:V5 tag under the influence of an inducible MtnA promoter were generated as a way to monitor secretion.

      (Bard et al., 2006)
      Parental Lines (1)
      Isolate of:
      Descendant Lines (0)
       
      External Stocks and Resources ( 3 )
      Crossreferences
      Cellosaurus - Cellosaurus is a knowledge resource on cell lines that attempts to describe all cell lines used in biomedical research.
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      RRID - Research Resource Identification Portal
      Synonyms and Secondary IDs (15)
      Reported As
      Symbol Synonym
      CVCL_Z992
      (Bairoch, 2016)
      DRSC 181
      (Rosin et al., 2018)
      RRID:CVCL_Z992
      (Bairoch, 2016)
      S2
      (Lu et al., 2025, Roy et al., 2025, Wood et al., 2025, Babosha et al., 2024, Barton et al., 2024, Brown et al., 2024, Drewell et al., 2024, Kaul et al., 2024, Platero et al., 2024, Tokamov et al., 2024, Zhang et al., 2024, Zhang et al., 2024, Duan et al., 2023, Klonaros et al., 2023, Liu et al., 2023, Ninova et al., 2023, Pinheiro et al., 2023, Tokamov et al., 2023, Ueda et al., 2023, Ventura Santos et al., 2023, Zhang et al., 2023, Faucillion et al., 2022, Garcia, 2022, Han et al., 2022, Imambocus et al., 2022, Martin et al., 2022, Richards et al., 2022, Wang et al., 2022, Erez et al., 2021, Goldman et al., 2021, Izadifar et al., 2021, Alabert et al., 2020, Barthez et al., 2020, Geigges et al., 2020, Laver et al., 2020, Lin et al., 2020, McLamarrah et al., 2020, Stergachis et al., 2020, Xu et al., 2020, Yoong et al., 2020, Albig et al., 2019, Chai et al., 2019, Flint Brodsly et al., 2019, Goldman et al., 2019, He et al., 2019, Moreira et al., 2019, Neal et al., 2019, Nil et al., 2019, Picchioni et al., 2019, Ramaekers et al., 2019, Suisse and Treisman, 2019, Yang et al., 2019, Lauwers et al., 2018, Lin et al., 2018, Rosin et al., 2018, Talbert et al., 2018, Ma et al., 2017, Pereira and Paro, 2017, Schauer et al., 2017, Urban et al., 2017, Zouaz et al., 2017, Adrian et al., 2016, Chlamydas et al., 2016, Huisinga et al., 2016, Ji and Tulin, 2016, Kwon et al., 2016, Nesler et al., 2016, Comoglio et al., 2015, Westholm et al., 2014, modENCODE Consortium et al., 2010, von Blume et al., 2009, Cherbas, 2008.11.12, Bard et al., 2006)
      S2 DRSC
      (Faucillion et al., 2022, Panhale et al., 2019)
      S2-DGSC
      (Fujinaga et al., 2025)
      S2-DRSC
      (Barton et al., 2024, Tokamov et al., 2024, Duan et al., 2023, Jneid et al., 2023, Klonaros et al., 2023, Ninova et al., 2023, Pizzano et al., 2023, Tokamov et al., 2023, Ventura Santos et al., 2023, Zhang et al., 2023, Garcia, 2022, Imambocus et al., 2022, Lewerentz et al., 2022, Mariyappa et al., 2022, Wang et al., 2022, Geigges et al., 2020, Stergachis et al., 2020, Yoong et al., 2020, Albig et al., 2019, Hu et al., 2019, Letizia et al., 2019, Luhur et al., 2019, Moreira et al., 2019, Suisse and Treisman, 2019, Zhang et al., 2019, Lauwers et al., 2018, Lin et al., 2018, Tan et al., 2018, Gerland et al., 2017, Janssens et al., 2017, Kim et al., 2017, Ma et al., 2017, Neukomm et al., 2017, Schauer et al., 2017, Umegawachi et al., 2017, Ammeux et al., 2016, Bairoch, 2016, Ji and Tulin, 2016, Kahn et al., 2016, Kwon et al., 2016, Stoiber et al., 2016, Webster et al., 2016, Comoglio et al., 2015, Lee et al., 2014, Seridi et al., 2014, Zheng et al., 2014, Kockmann et al., 2013, Cherbas et al., 2011, Cherbas, 2008.11.12)
      S2-DRSC-1
      (Han et al., 2022)
      S2-DRSC-2
      (Han et al., 2022)
      S2-DRSC-3
      (Han et al., 2022)
      S2_DRSC
      (Cloud et al., 2019)
      SL2
      (Cherbas, 2008.11.12)
      Schneider's 2
      (Cherbas, 2008.11.12)
      Schneider's Line S2
      (Thakur and O'Connor-Giles, 2025)
      Schneider's line 2
      (Cherbas, 2008.11.12)
      Name Synonyms
      Secondary FlyBase IDs
        References (183)