A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dhyd\Minos

General Information
Symbol Dhyd\Minos Species D.hydei
Name Minos element FlyBase ID FBte0000026
Feature type natural transposable element
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Description
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FB2013_03
FB2013_02
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hide Sequences & Components
Complete element (bp)
1775
 
Terminal repeat (bp)
255
 
Reference sequence transposon_sequence_set.embl.txt.gz
Component genes
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Transposon type
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Copy number
and comments
to 30
 
Search for
Target Site Duplication
Size (bp)
0 or 1
 
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Curated drosophilid orthologs
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Mi{ET1} insertions in D. melanogaster are over-represented in both testis-specific and all other loci within underreplicated regions of the genome (URs) compared to flanks; this pattern is the opposite of that observed with insertions of P-element and Tni\piggyBac transgenic constructs.
A defective Dhyd\Minos transposon that is inserted into the D.melanogaster genome is stable in the absence of Dhyd\Minos\T but is mobilised at relatively high frequencies in the presence of transposase. Transposase can induce transposition of defective Dhyd\Minos to a new location. Analysis of structures remaining after excision suggests the breaks can be repaired by two alternative mechanisms: gap repair or ligation repair.
Precise excisions can be recovered only from heterozygotes, and not from hemi- or homozygotes. Footprints of 6 nucleotides are generally left in excisions from hemi- or homozygotes and are left at a frequency of 25% in excisions from heterozygotes. The gap left after Dhyd\Minos excision might be repaired by an alternative mechanism not involving gene conversion and the structure of the repaired donor sites might, in some way, reflect the mechanism of action of Dhyd\Minos transposase.
Dhyd\Minos elements actively transpose in the germ line, and are characterised by a striking degree of sequence and size homogeneity. Elements insert in a TA nucleotide that is probably duplicated during the process.
A Dhyd\Minos element has been cloned and sequenced.
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hide Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym
Dhyd\Minos
 
Name Synonym
Minos element
 
Secondary FlyBase IDs
  • FBgn0004585
  • FBgn0010242
  • FBtp0011478
hide References ( 48 )
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hide Recent research papers ( 5 )
Gummalla et al., 2012, PLoS Genet. 8(5): e1002720
abd-A Regulation by the iab-8 Noncoding RNA. [FBrf0218472]
Kane et al., 2012, PLoS Genet. 8(4): e1002659
Competition between Replicative and Translesion Polymerases during Homologous Recombination Repair in Drosophila. [FBrf0218093]
Egoz-Matia et al., 2011, Dev. Biol. 351(1): 99--109
Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression. [FBrf0213026]
Spradling et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(38): 15948--15953
Drosophila P elements preferentially transpose to replication origins. [FBrf0215815]
Venken et al., 2011, Nat. Methods 8(9): 737--743
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331]
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All reviews listed in FlyBase were published before 2011