Dmel\P{lacZ-un1}loco^rC56 Insertion

General Information
Symbol	Dmel\P{lacZ-un1}loco^rC56	Species	D. melanogaster
Name		FlyBase ID	FBti0000730
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacZ-un1}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, loco	Viability / fertility
Causes allele(s)	Ecol\lacZ^loco-rC56, loco^rC56	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 94B7 )	Sequence location	3R:18,452,336..18,452,689 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:18,452,336..18,452,689 [-] (Granderath et al., 1999)
Orientation	- (Granderath et al., 1999)
Cytological location (computed by FlyBase)	94B7 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location	The P{lacZ-un1}loco[rC56] insertion site maps between an EcoRI site and the loco-RC transcription start and is reported to be within 1 bp of P{lacZ}loco[3-109]. (Granderath et al., 1999)
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacZ-un1}
Location-dependent role	lacZ enhancer trap (FlyBase, 1992-)
Size
Associated alleles	ry^+t*
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Menne and Klambt, 1994, Yuasa et al., 2003, Shandala et al., 2003, Granderath et al., 2000, Granderath et al., 1999, Kretzschmar et al., 1997, Pielage et al., 2004, Klambt, 1998.8.10, Bernardoni et al., 1998, Wemmer and Klaembt, 1995, Thomas and van Meyel, 2007, Kaplow et al., 2008, Ding et al., 2011) loco (Granderath et al., 1999, Granderath et al., 1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^loco-rC56 (Menne and Klambt, 1994, Yuasa et al., 2003, Shandala et al., 2003, Granderath et al., 2000, Granderath et al., 1999, Kretzschmar et al., 1997, Pielage et al., 2004, Klambt, 1998.8.10, Bernardoni et al., 1998, Wemmer and Klaembt, 1995, Thomas and van Meyel, 2007, Kaplow et al., 2008, Ding et al., 2011) loco^rC56 (Granderath et al., 1999)

Lethality References viable (Klaembt, 1995.10.24)
Sterility References fertile (Klaembt, 1995.10.24)
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage CNS glial cell \| subset (Menne and Klambt, 1994) lateral ectoderm \| dorsal (Granderath et al., 1999) embryonic stage 12 interface glial cell (Klaes et al., 1994) glial cell of VUM neuron (Klaes et al., 1994) embryonic stage 12 -- 17 glial cell (Granderath et al., 1999) embryonic stage 13 -- 17 segmental nerve root glial cell (Klaes et al., 1994) exit glial cell (Klaes et al., 1994) B-subperineurial glial cell of abdomen (Klaes et al., 1994) A-subperineurial glial cell (Klaes et al., 1994) medial intersegmental nerve root glial cell (Klaes et al., 1994) embryonic stage 16 lateral cord glial cell (Granderath et al., 2000)
Additional Information
Statement Reference Enhancer trap expression is detected at embryonic stage 12 in cells which appear to be progeny of the lateral glioblast. Those cells migrate medially and divide. By the end of embryogenesis, enhancer trap expression is observed in most glial cells except the midline glia. Expression is also seen in the dorsal leading edge cells of the lateral ectoderm. (Granderath et al., 1999)

Marker for	lateral cord glia (Granderath et al., 2000)
Reflects expression of	loco (Granderath et al., 1999)
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	10009 P{lacZ-un1}loco^rC56
Linkouts
Comments
	The P{lacZ-un1}loco^rC56 insertion site maps between an EcoRI site and the loco-RC transcription start and is reported to be within 1 bp of P{lacZ}3-109. It is in a positive orientation with respect to loco gene transcription. (Granderath et al., 1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	loco-lacZ (Thomas and van Meyel, 2007, Ding et al., 2011) P{A92}rC56 (FlyBase, 1992-) P{lacZ}rC56 (FlyBase, 1992-) P{lacZ-un1}loco^rC56 (FlyBase, 1992-, Granderath et al., 1999, Kaplow et al., 2008, ) P{lacZ-un1}rC56 (Menne and Klambt, 1994, FlyBase, 1992-) P{PZ}rC56 (FlyBase, 1992-) P{ry^+t7.2=PZ}rC56 (FlyBase, 1992-) rC56 (Pielage et al., 2004, Klaes et al., 1994) rc56 (Shandala et al., 2003, Granderath et al., 2000)
Secondary FlyBase IDs
	FBti0002154 FBti0009198
References ( 17 )
Research paper	Ding et al., 2011, Dev. Dyn. 240(1): 122--134 Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila. [FBrf0212620] Kaplow et al., 2008, Genetics 178(4): 2003--2016 Regulation of Glia Number in Drosophila by Rap/Fzr, an Activator of the Anaphase-Promoting Complex, and Loco, an RGS Protein. [FBrf0204641] Thomas and van Meyel, 2007, Development 134(3): 591--600 The glycosyltransferase Fringe promotes Delta-Notch signaling between neurons and glia, and is required for subtype-specific glial gene expression. [FBrf0192828] Pielage et al., 2004, Dev. Biol. 275(5): 245--257 The Drosophila transmembrane protein Fear-of-intimacy controls glial cell migration. [FBrf0180105] Shandala et al., 2003, Development 130(8): 1505--1513 The dead ringer/retained transcriptional regulatory gene is required for positioning of the longitudinal glia in the Drosophila embryonic CNS. [FBrf0158873] Yuasa et al., 2003, Development 130(11): 2419--2428 Drosophila homeodomain protein REPO controls glial differentiation by cooperating with ETS and BTB transcription factors. [FBrf0158889] Granderath et al., 2000, Mech. Dev. 91(1-2): 197--208 gcm and pointed synergistically control glial transcription of the Drosophila gene loco. [FBrf0127098] Granderath et al., 1999, Development 126(8): 1781--1791 loco encodes an RGS protein required for Drosophila glial differentiation. [FBrf0108191] Bernardoni et al., 1998, Development 125(16): 3189--3200 Glial differentiation does not require a neural ground state. [FBrf0104717] Kretzschmar et al., 1997, J. Neurosci. 17(19): 7425--7432 The swiss cheese mutant causes glial hyperwrapping and brain degeneration in Drosophila. [FBrf0098827] Wemmer and Klaembt, 1995, Genetics 140(2): 629--641 A genetic analysis of the Drosophila closely linked interacting genes bulge, argos and soba. [FBrf0082770] Klaes et al., 1994, Cell 78(1): 149--160 The Ets transcription factors encoded by the Drosophila gene pointed direct glial cell differentiation in the embryonic CNS. [FBrf0073570] Menne and Klambt, 1994, Development 120(1): 123--133 The formation of commissures in the Drosophila CNS depends on the midline cells and on the Notch gene. [FBrf0068598]
Personal communication to FlyBase	Klambt, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103139] Klaembt, 1996.12.4, Df(3R)23D1 and Df(3R)5C1. Df(3R)23D1 and Df(3R)5C1. [FBrf0091283] Klaembt, 1995.10.24, P{lacZ-un1}loco[rC56] and P{lwB}AA142. P{lacZ-un1}loco[rC56] and P{lwB}AA142. [FBrf0122648]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacZ-un1}locorC56 Insertion

Dmel\P{lacZ-un1}loco^rC56 Insertion