FlyBase Insertion Report: Dmel\P{en1}wg[en11]

General Information

Symbol

Dmel\P{en1}wg^en11

Species

D. melanogaster

Name

FlyBase ID

FBti0002065

Feature type

transposable_element_insertion_site

Also Known As

Wg-lacZ, wingless-lacZ, wglacZ, wg-Z, wgZ, P{en1}wgen11, wingless^en11

Description

Inserted Element

P{en1}

Uses

lacZ reporter

Affected gene(s)

Ecol\lacZ, wg

Viability / fertility

Associated allele(s)

Ecol\lacZ^wg-en11, wg^en11

Stock availability

53 publicly available

LINE ID

Genomic Location

Chromosomal Location

2L ( 27F1 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

27F1 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{en1}

Location-dependent role

lacZ enhancer trap

(Kassis, 1990)

Size (Kb)

15.063

Associated alleles

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Avanesov et al., 2012, Chen and Verheyen, 2012, Troost et al., 2012, Kamimura et al., 2011, Olson et al., 2011, Quijano et al., 2011, Kleinschmit et al., 2010, Mukai et al., 2010, Padash-Barmchi et al., 2010, Dichtel-Danjoy et al., 2009, Grieder et al., 2009, Beckervordersandforth et al., 2008, Brás-Pereira and Casares, 2008, Mitchell et al., 2008, Nusinow et al., 2008, Zirin and Mann, 2007, Bras-Pereira et al., 2006, Dussillol-Godar et al., 2006, Ekas et al., 2006, Herranz et al., 2006, Jaekel and Klein, 2006, Lim and Tomlinson, 2006, Matakatsu and Blair, 2006, Pereira et al., 2006, Singh et al., 2006, Kenyon et al., 2005, Miskolczi-McCallum et al., 2005, Yao and Sun, 2005, Baeg et al., 2004, Costas et al., 2004, De Graeve et al., 2004, Grabbe et al., 2004, Hori et al., 2004, Lanzotti et al., 2004, Niwa et al., 2004, Wojtowicz et al., 2004, Baena-Lopez and Garcia-Bellido, 2003, Estella et al., 2003, Kolzer et al., 2003, Kubota et al., 2003, Urbach and Technau, 2003, Whitworth and Russell, 2003, Baonza and Freeman, 2002, Cadigan et al., 2002, del Alamo Rodriguez et al., 2002, Erkner et al., 2002, Lockwood and Bodmer, 2002, Lo et al., 2002, Mirth and Akam, 2002, Nakagoshi et al., 2002, Sato and Kornberg, 2002, Shao et al., 2002, Soustelle et al., 2002, Zecca and Struhl, 2002, Gieseler et al., 2001, Hatini and DiNardo, 2001, Herzig et al., 2001, Lee and Treisman, 2001, Mozer, 2001, Stewart et al., 2001, Treisman, 2001, Azpiazu and Morata, 2000, Calleja et al., 2000, Casares and Mann, 2000, Cavodeassi et al., 2000, Certel et al., 2000, Cho et al., 2000, Klein et al., 2000, Maurel-Zaffran and Treisman, 2000, Miletich and Limbourg-Bouchon, 2000, Mukherjee et al., 2000, Pfeiffer et al., 2000, Pichaud and Casares, 2000, Rogina and Helfand, 2000, Russell, 2000, Wang et al., 2000, Amin et al., 1999, Apidianakis et al., 1999, Brennan et al., 1999, Cavodeassi et al., 1999, Gorfinkiel et al., 1999, Goto and Hayashi, 1999, Gritzan et al., 1999, Kiger et al., 1999, Klein and Martinez Arias, 1999, Ligoxygakis et al., 1999, Lin and Perrimon, 1999, Moreno and Morata, 1999, Rincon-Limas et al., 1999, Sato et al., 1999, Shashidhara et al., 1999, Varadarajan and VijayRaghavan, 1999, Wessells et al., 1999, Ye et al., 1999, Bachmann and Knust, 1998, Borod and Heberlein, 1998, Castelli-Gair, 1998, Damm et al., 1998, Fernandez-Funez et al., 1998, Gallitano-Mendel and Finkelstein, 1998, Go et al., 1998, Hazelett et al., 1998, Heberlein et al., 1998, Karim and Rubin, 1998, Klein and Martinez Arias, 1998, Klein and Martinez Arias, 1998, Klein et al., 1998, Nakagoshi et al., 1998, Nicholls and Gelbart, 1998, Paumard-Rigal et al., 1998, Rodriguez-Esteban et al., 1998, Saget et al., 1998, Theodosiou et al., 1998, Zeng et al., 1998, Zhang and Carthew, 1998, Casares et al., 1997, Chanut and Heberlein, 1997, de Celis and Bray, 1997, Dominguez and Hafen, 1997, Gallitano-Mendel and Finkelstein, 1997, Gorfinkiel et al., 1997, Hepker et al., 1997, Heslip et al., 1997, Micchelli et al., 1997, O'Keefe et al., 1997, Pignoni and Zipursky, 1997, Rogina and Helfand, 1997, Rogina et al., 1997, Sanchez et al., 1997, Spradling et al., 1997, Struhl et al., 1997, Adamson and Shearn, 1996, Brook and Cohen, 1996, Forbes et al., 1996, Freeland and Kuhn, 1996, Gonczy and DiNardo, 1996, Jonsson and Knust, 1996, Morimura et al., 1996, Neumann and Cohen, 1996, Neumann and Cohen, 1996, Newfeld et al., 1996, Ng et al., 1996, Penton and Hoffmann, 1996, Royet and Finkelstein, 1996, Shirras and Couso, 1996, Zecca et al., 1996, Boyle and DiNardo, 1995, Broadus et al., 1995, de Celis et al., 1995, Diaz-Benjumea and Cohen, 1995, Lawrence et al., 1995, Lecourtois and Schweisguth, 1995, Ma and Moses, 1995, Matthews, 1995.11.17, Raftery et al., 1995, Thomas et al., 1995, Treisman and Rubin, 1995, Yoffe et al., 1995, Arora et al., 1994, Basler and Struhl, 1994, Lee et al., 1994, Couso et al., 1993, Forbes et al., 1993, Kassis et al., 1992, Schmidt-Ott and Technau, 1992, Skaer and Martinez Arias, 1992, Ingham et al., 1991, Finkelstein and Perrimon, 1990)

(Sánchez-Higueras et al., 2019, Kassis et al., 1992, Ingham et al., 1991)

Alleles and Phenotypes

Allele

Ecol\lacZ^wg-en11

Reference(s)

(Sánchez-Higueras et al., 2019, Domínguez-Cejudo and Casares, 2015, Reilly et al., 2015, Won et al., 2015, Mouri et al., 2014, Pitsouli and Perrimon, 2013, Avanesov et al., 2012, Chen and Verheyen, 2012, Troost et al., 2012, Kamimura et al., 2011, Olson et al., 2011, Quijano et al., 2011, Kleinschmit et al., 2010, Mukai et al., 2010, Padash-Barmchi et al., 2010, Dichtel-Danjoy et al., 2009, Grieder et al., 2009, Beckervordersandforth et al., 2008, Brás-Pereira and Casares, 2008, Mitchell et al., 2008, Nusinow et al., 2008, von Hilchen et al., 2008, Sprecher et al., 2007, Zirin and Mann, 2007, Bras-Pereira et al., 2006, Dussillol-Godar et al., 2006, Ekas et al., 2006, Herranz et al., 2006, Jaekel and Klein, 2006, Lim and Tomlinson, 2006, Matakatsu and Blair, 2006, Pereira et al., 2006, Singh et al., 2006, Kenyon et al., 2005, Miskolczi-McCallum et al., 2005, Yao and Sun, 2005, Baeg et al., 2004, Costas et al., 2004, De Graeve et al., 2004, Grabbe et al., 2004, Hori et al., 2004, Lanzotti et al., 2004, Niwa et al., 2004, Wojtowicz et al., 2004, Baena-Lopez and Garcia-Bellido, 2003, Estella et al., 2003, Kolzer et al., 2003, Kubota et al., 2003, Urbach and Technau, 2003, Whitworth and Russell, 2003, Baonza and Freeman, 2002, Cadigan et al., 2002, del Alamo Rodriguez et al., 2002, Erkner et al., 2002, Lockwood and Bodmer, 2002, Lo et al., 2002, Mirth and Akam, 2002, Nakagoshi et al., 2002, Sato and Kornberg, 2002, Shao et al., 2002, Soustelle et al., 2002, Zecca and Struhl, 2002, Gieseler et al., 2001, Hatini and DiNardo, 2001, Herzig et al., 2001, Lee and Treisman, 2001, Mozer, 2001, Stewart et al., 2001, Treisman, 2001, Azpiazu and Morata, 2000, Calleja et al., 2000, Casares and Mann, 2000, Cavodeassi et al., 2000, Certel et al., 2000, Cho et al., 2000, Klein et al., 2000, Maurel-Zaffran and Treisman, 2000, Miletich and Limbourg-Bouchon, 2000, Mukherjee et al., 2000, Pfeiffer et al., 2000, Pichaud and Casares, 2000, Rogina and Helfand, 2000, Russell, 2000, Wang et al., 2000, Amin et al., 1999, Apidianakis et al., 1999, Brennan et al., 1999, Cavodeassi et al., 1999, Gorfinkiel et al., 1999, Goto and Hayashi, 1999, Gritzan et al., 1999, Kiger et al., 1999, Klein and Martinez Arias, 1999, Ligoxygakis et al., 1999, Lin and Perrimon, 1999, Moreno and Morata, 1999, Rincon-Limas et al., 1999, Sato et al., 1999, Shashidhara et al., 1999, Varadarajan and VijayRaghavan, 1999, Wessells et al., 1999, Ye et al., 1999, Bachmann and Knust, 1998, Borod and Heberlein, 1998, Castelli-Gair, 1998, Damm et al., 1998, Fernandez-Funez et al., 1998, Gallitano-Mendel and Finkelstein, 1998, Go et al., 1998, Hazelett et al., 1998, Heberlein et al., 1998, Karim and Rubin, 1998, Klein and Martinez Arias, 1998, Klein and Martinez Arias, 1998, Klein et al., 1998, Nakagoshi et al., 1998, Nicholls and Gelbart, 1998, Paumard-Rigal et al., 1998, Rodriguez-Esteban et al., 1998, Saget et al., 1998, Theodosiou et al., 1998, Zeng et al., 1998, Zhang and Carthew, 1998, Casares et al., 1997, Chanut and Heberlein, 1997, de Celis and Bray, 1997, Dominguez and Hafen, 1997, Gallitano-Mendel and Finkelstein, 1997, Gorfinkiel et al., 1997, Hepker et al., 1997, Heslip et al., 1997, Micchelli et al., 1997, O'Keefe et al., 1997, Pignoni and Zipursky, 1997, Rogina and Helfand, 1997, Rogina et al., 1997, Sanchez et al., 1997, Spradling et al., 1997, Struhl et al., 1997, Adamson and Shearn, 1996, Brook and Cohen, 1996, Forbes et al., 1996, Freeland and Kuhn, 1996, Gonczy and DiNardo, 1996, Jonsson and Knust, 1996, Morimura et al., 1996, Neumann and Cohen, 1996, Neumann and Cohen, 1996, Newfeld et al., 1996, Ng et al., 1996, Penton and Hoffmann, 1996, Royet and Finkelstein, 1996, Shirras and Couso, 1996, Zecca et al., 1996, Boyle and DiNardo, 1995, Broadus et al., 1995, de Celis et al., 1995, Diaz-Benjumea and Cohen, 1995, Lawrence et al., 1995, Lecourtois and Schweisguth, 1995, Ma and Moses, 1995, Matthews, 1995.11.17, Raftery et al., 1995, Thomas et al., 1995, Treisman and Rubin, 1995, Yoffe et al., 1995, Arora et al., 1994, Basler and Struhl, 1994, Lee et al., 1994, Couso et al., 1993, Forbes et al., 1993, Kassis et al., 1992, Schmidt-Ott and Technau, 1992, Skaer and Martinez Arias, 1992, Ingham et al., 1991, Finkelstein and Perrimon, 1990)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Allele

wg^en11

Reference(s)

(Sánchez-Higueras et al., 2019, Cox et al., 2005, Yoshikawa et al., 2003, Conley et al., 2000, Sato et al., 1999, Klein and Martinez Arias, 1998, Buratovich et al., 1997, Haerry et al., 1997, Li et al., 1995, Perrimon, 1995.8.30, Rulifson and Blair, 1995, Yoffe et al., 1995, Heemskerk and DiNardo, 1994, Hing et al., 1994, Kassis et al., 1992, Schmidt-Ott and Technau, 1992)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic proventriculus | restricted

(Nakagoshi et al., 2002)

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

dorsal-ventral compartment boundary of the wing disc

(Casares and Mann, 2000)

wing hinge primordium

(Casares and Mann, 2000)

presumptive tegula

(Casares and Mann, 2000)

third instar larval stage

eye disc | restricted

(Hazelett et al., 1998)

female genital disc | restricted

(Freeland and Kuhn, 1996)

larval abdominal segment | anterior

(Struhl et al., 1997)

histoblast | subset

(Struhl et al., 1997)

male genital disc | restricted

(Freeland and Kuhn, 1996)

wing disc | restricted

(Thomas et al., 1995)

embryonic stage

embryonic proventriculus | restricted

(Gorfinkiel et al., 1999)

Malpighian tubule

(Skaer and Martinez Arias, 1992)

embryonic stage 10 -- 17

ventral nerve cord

(Matthews, 1995.11.17)

embryonic/larval proventriculus

(Matthews, 1995.11.17)

embryonic/larval midgut of parasegment 8

(Matthews, 1995.11.17)

epidermis | striped

(Matthews, 1995.11.17)

larval stage

eye-antennal disc | restricted

(Mukherjee et al., 2000)

ventral thoracic disc | restricted

(Mukherjee et al., 2000)

antennal disc | restricted

(Mukherjee et al., 2000)

eye disc | lateral | restricted

(Mukherjee et al., 2000)

adult stage

adult abdominal segment | anterior

(Struhl et al., 1997)

tegula

(Casares and Mann, 2000)

adult midgut circular muscle

(Lin et al., 2008)

embryonic stage 11

ectoderm | segmentally repeated

(Lawrence et al., 1995)

embryonic stage 13 -- 17

embryonic peripheral glial cell 2

(von Hilchen et al., 2008)

embryonic peripheral glial cell 4

(von Hilchen et al., 2008)

embryonic peripheral glial cell 5

(von Hilchen et al., 2008)

embryonic peripheral glial cell 10

(von Hilchen et al., 2008)

embryonic peripheral glial cell 11

(von Hilchen et al., 2008)

second instar larval stage

disc epithelium proper of wing disc | restricted | ventral

Comment: ventral wedge of expression.

(Nusinow et al., 2008)

Additional Information

Statement

Reference

In second instar wing discs, wg expression, as assayed by Ecol\lacZ^wg-en11 expression, accumulates in the disc epithelium proper in a wedge of ventro-anterior cells. At early to mid third instar, Ecol\lacZ^wg-en11 begins to exhibit expression along the dorsal/ventral compartment boundary of the wing disc.

(Nusinow et al., 2008)

Reporter expression is detected is detected in the circular muscles that envelope gut epithelia.

(Lin et al., 2008)

Expression in the leg and antennal discs is observed in a wedge in the anterior/ventral quadrant, and in the lateral regions of the eye disc.

(Mukherjee et al., 2000)

Expression in the male and female genital discs is complementary to that of dpp.

(Gorfinkiel et al., 1999)

In third instar larval abdominal segments, expressed cells of the anterior compartment immmediately anterior to the posterior compartment, and in histoblasts within the anterior compartment. In adult abdominal segments, expressed in the anterior compartment, with highest levels at posterior edge.

(Struhl et al., 1997)

Strong embryonic expression in epidermal stripes, midgut in parasegment 8 and in proventriculus; weak expression in ventral nerve cord.

(Matthews, 1995.11.17)

Ecol\lacZ reporter gene expression isseen in all of the cells of the Malpighian tubules as they evert from theproctodeum. Staining becomes faint on the anterior side of the tubules andin the tip cells during germ band shortening suggesting that expressionbecomes limited to the cells on the posterior side of the tubules asdevelopment proceeds.

(Skaer and Martinez Arias, 1992)

Marker for

dorsal-ventral compartment boundary of the wing disc

(Casares and Mann, 2000)

embryonic peripheral glial cell 10

(von Hilchen et al., 2008)

embryonic peripheral glial cell 11

(von Hilchen et al., 2008)

embryonic peripheral glial cell 2

(von Hilchen et al., 2008)

embryonic peripheral glial cell 4

(von Hilchen et al., 2008)

embryonic peripheral glial cell 5

(von Hilchen et al., 2008)

Reflects expression of

(Lin et al., 2008, Nusinow et al., 2008, Nakagoshi et al., 2002, Casares and Mann, 2000, Mukherjee et al., 2000, Gorfinkiel et al., 1999, Hazelett et al., 1998, Struhl et al., 1997, Freeland and Kuhn, 1996, Lawrence et al., 1995, Matthews, 1995.11.17, Thomas et al., 1995, Schmidt-Ott and Technau, 1992)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

CyO-P{en1}wg^en11

(FlyBase Curators, 2006-, Matthews, 1995.11.17, Perrimon, 1995.8.30, Wu et al., 1995, Yoffe et al., 1995, Heemskerk and DiNardo, 1994, Kassis et al., 1992)

CyO-vAg

(FlyBase Curators, 2006-)

Stocks (53)

Bloomington

50763

tsh⁸/CyO, P{en1}wg^en11

30720

ebi^E90/CyO, P{en1}wg^en11

59061

CycE^5L/CyO, P{en1}wg^en11

51661

w^*; P{hs-N.icd}2/CyO, P{en1}wg^en11