Dmel\P{neoFRT}18A Insertion

General Information
Symbol	Dmel\P{neoFRT}18A	Species	D. melanogaster
Name		FlyBase ID	FBti0002070
Feature type	transposable_element_insertion_site	Created / Updated	1997-03-21/1998-01-31
Description
Inserted element	P{neoFRT}	Localized function
Affected gene(s)		Expression data
Causes allele(s)		Viability / fertility
LINE ID		Stock availability
Genomic Location
Chromosomal location	X ( 18A )	Sequence location
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)	18A (in situ hybridization reported) (Xu and Rubin, 1993)
Chromosome in situ
Sequence Data
Flanking sequence
Inserted Element
Construct	P{neoFRT}
Location-dependent role
Size	15.262Kb
Associated alleles	ry^+t7.2 Tn\neoR^Hsp70Bb.PS
Molecular map
Transposon class
Element type
Affected Gene(s)
Insertion within gene
Insertion may affect gene
Orientation (relative to gene)	Gene Orientation References
Alleles and Phenotypes
Causes alleles

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 26 )
Bloomington	1578 w¹¹¹⁸ P{neoFRT}18A 1054 P{neoFRT}18A; ry⁵⁰⁶ 1526 w¹¹¹⁸ P{NM}8A P{neoFRT}18A 1472 w¹¹¹⁸ P{πM}10D P{neoFRT}18A 1504 B¹ P{neoFRT}18A; ry⁵⁰⁶ 1481 w¹¹¹⁸ P{πM}5A P{πM}10D P{neoFRT}18A 7370 w¹¹¹⁸ P{arm-lacZ.V}MM1 P{neoFRT}18A
Kyoto	101206 w¹¹¹⁸ P{neoFRT}18A 106397 w¹¹¹⁸ P{neoFRT}18A 106366 w¹¹¹⁸ P{NM}8A P{neoFRT}18A 106330 w¹¹¹⁸ P{πM}10D P{neoFRT}18A 106351 B^1? P{neoFRT}18A; ry⁵⁰⁶
	More stocks available...
Linkouts
Comments
	In the presence of continuous Scer\FLP1, P{neoFRT}18A is >75% lethal and 100% of the escapers have severe eye and head defects. The lethality and developmental abnormalities are observed with both male and female flies carrying an P{neoFRT}18A chromosome, so the defects are not due to inter-chromosomal recombination. The P{neoFRT}18A chromosome may carry an aberration (perhaps another FRT site near the one at 18A) and that this has been transmitted to recombinant chromosomes carrying P{neoFRT}18A. I further suspect that recombination between the two FRT sites deletes essential genes, leading to cell lethality and head defects. Wing disc development is disrupted in flies carrying P{neoFRT}18A and Scer\GAL4^ap-md544, a continuous source of Scer\FLP1 in the wing. Thus P{neoFRT}18A probably is cell lethal in the presence of continuous Scer\FLP1. (Ebacher, 2002.7.31) Used to generate mosaics mediated by FLP recombinase; P{neoFRT} insertion at proximal location in chromosome arm. (Xu and Rubin, 1993)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[ry⁺; hs-neo; FRT]18A (Xu and Rubin, 1993) P{neoFRT}18A (Ebacher, 2002.7.31, FlyBase, 1992-) P{ry^+t7.2=neoFRT}18A (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000869
References ( 3 )
Research paper	Xu and Rubin, 1993, Development 117(4): 1223--1237 Analysis of genetic mosaics in developing and adult Drosophila tissues. [FBrf0058070]
Personal communication to FlyBase	Ebacher, 2002.7.31, P{neoFRT}18A. P{neoFRT}18A. [FBrf0150864]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]