Dmel\P{lacW}ci^Dplac Insertion

General Information
Symbol	Dmel\P{lacW}ci^Dplac	Species	D. melanogaster
Name		FlyBase ID	FBti0002268
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	ci, Ecol\lacZ	Viability / fertility
Causes allele(s)	ci^Dplac, ci^E1, ci^E2, Ecol\lacZ^ci-Dplac	Stock availability	2 publicly available
LINE ID
Genomic Location
Chromosomal location	4 ( -101F1 )	Sequence location
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10	References Jia et al., 2010, J. Biol. Chem. 285(48): 37218--37226
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	-101F1 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	ci (Eaton and Kornberg, 1990) Ecol\lacZ (Eaton and Kornberg, 1990, Basler and Struhl, 1994, Major and Irvine, 2005, Apidianakis et al., 2001, Jia et al., 2002, Ou et al., 2002, Pan and Rubin, 1995, Bai and Montell, 2002, Alcedo et al., 2000, Svendsen et al., 2000, Blair, 1992, Bushey and Locke, 2004, Singh and Gopinathan, 1998, Saget et al., 1998, Strutt and Mlodzik, 1996, Struhl et al., 1997, Locke and Hanna, 1996, Forbes et al., 1996, Jiang and Struhl, 1998, Bejarano et al., 2007, Kim et al., 2010, Jia et al., 2010)
Alleles and Phenotypes
Causes alleles	ci^Dplac (Eaton and Kornberg, 1990, Micchelli et al., 2002, Locke and McDermid, 1993, Slusarski et al., 1995, Blair, 1992) ci^E1 (Bushey and Locke, 2004) ci^E2 (Bushey and Locke, 2004) Ecol\lacZ^ci-Dplac (Eaton and Kornberg, 1990, Basler and Struhl, 1994, Major and Irvine, 2005, Apidianakis et al., 2001, Jia et al., 2002, Ou et al., 2002, Pan and Rubin, 1995, Bai and Montell, 2002, Alcedo et al., 2000, Svendsen et al., 2000, Blair, 1992, Bushey and Locke, 2004, Singh and Gopinathan, 1998, Saget et al., 1998, Strutt and Mlodzik, 1996, Struhl et al., 1997, Locke and Hanna, 1996, Forbes et al., 1996, Jiang and Struhl, 1998, Bejarano et al., 2007, Kim et al., 2010, Jia et al., 2010)

Lethality References lethal \| embryonic stage \| recessive (Eaton and Kornberg, 1990)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Semilethal over ci^Ce-2, most die as embryos, adult survivors appear normal. Heterozygotes with ci^D die around eclosion having abnormal legs and mouthparts. (Eaton and Kornberg, 1990)
Expression Data
Reporter Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference larval stage imaginal disc \| posterior (Eaton and Kornberg, 1990) distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage organism \| segment polarity expression pattern (Eaton and Kornberg, 1990) larval stage wing disc \| anterior compartment (Blair, 1992) pupal stage wing \| anterior compartment (Blair, 1992)
Additional Information
Statement Reference In pupal wing, expression in anterior compartment not uniform: slightly fainter in cells midway between L3 and L4 down to the AP lineage boundary. (Blair, 1992) Enhancer trap staining occurs in wide,segmentally repeated stripes in embryos and in imaginal discs. In bothembryos and imaginal discs, expression was found to be in the anteriorcompartments and precisely complements en expression. In engrailedmutants, Ecol\lacZ expression is derepressed in the posterior compartments inembryos and imaginal discs, suggesting that ci expression is normallyrepressed by en in these cells. (Eaton and Kornberg, 1990)

Marker for
Reflects expression of	ci (Eaton and Kornberg, 1990, Blair, 1992)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration	T(2;4)DB47F (Bushey and Locke, 2004) T(2;4)DB57A (Bushey and Locke, 2004)
Balancer
Stocks ( 2 )
Bloomington	6303 y¹ w^*; P{lacW}ci^Dplac
Kyoto	101933 y¹ ; P{lacW}ci^Dplac
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	ci^D-lacZ (Alcedo et al., 2000) ci-lacZ (Major and Irvine, 2005, Ou et al., 2002, Bai and Montell, 2002, Jia et al., 2002, Apidianakis et al., 2001, Svendsen et al., 2000, Bejarano et al., 2007, Jia et al., 2010) Ci-lacZ (Kim et al., 2010) P[lacW]ci^Dplac (Apidianakis et al., 2001) P{lacW}ci^Dplac (Bushey and Locke, 2004, FlyBase, 1992-) P{lacZ-un2}ci^Dplac (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0002684
References ( 27 )

Generate a list of	All references relating to this insertion ( 27 )

List References by type	Research paper ( 25 ) Review ( 1 ) FlyBase analysis ( 1 )
Recent research papers ( 2 )
	Jia et al., 2010, J. Biol. Chem. 285(48): 37218--37226 Casein kinase 2 promotes Hedgehog signaling by regulating both smoothened and Cubitus interruptus. [FBrf0214177] Kim et al., 2010, Dev. Biol. 346(2): 247--257 Drosophila Cand1 regulates Cullin3-dependent E3 ligases by affecting the neddylation of Cullin3 and by controlling the stability of Cullin3 and adaptor protein. [FBrf0211875] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...

Dmel\P{lacW}ciDplac Insertion

Dmel\P{lacW}ci^Dplac Insertion