Dmel\P{lacZ-un4}hkb⁵⁹⁵³ Insertion

General Information
Symbol	Dmel\P{lacZ-un4}hkb⁵⁹⁵³	Species	D. melanogaster
Name		FlyBase ID	FBti0002357
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacZ-un4}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, hkb	Viability / fertility	viable
Causes allele(s)	Ecol\lacZ^hkb-5953, hkb⁵⁹⁵³	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 82A4 )	Sequence location
Recent Updates
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FB2012_01	Controlled Vocabulary Terms ventro-lateral serotonergic neuron References Lundell et al., 1996, Molec. Cell. Neurosci. 7(1): 46--61
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	82A4 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacZ-un4}
Location-dependent role	lacZ enhancer trap (FlyBase, 1992-)
Size
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Andrew et al., 1994, Mehta and Bhat, 2001, Bhat et al., 2000, Urbach and Technau, 2003, Ragone et al., 2003, Doe and Technau, 1993, Goodman and Doe, 1993, Chu-LaGraff and Doe, 1993, Buescher et al., 2002, De Graeve et al., 2004, Isshiki et al., 1997, Schmidt et al., 1997, Doe, 1992, Bhat, 1998, Chu et al., 1998, Bossing et al., 1996, Broadus et al., 1995, Bhat, 1996, Matsuzaki and Saigo, 1996, Bhat and Schedl, 1997, Buescher and Chia, 1997, Baumgardt et al., 2007, Losada-Pérez et al., 2010, Beckervordersandforth et al., 2008, Lundell et al., 1996) hkb (Andrew et al., 1994)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^hkb-5953 (Andrew et al., 1994, Mehta and Bhat, 2001, Bhat et al., 2000, Urbach and Technau, 2003, Ragone et al., 2003, Doe and Technau, 1993, Goodman and Doe, 1993, Chu-LaGraff and Doe, 1993, Buescher et al., 2002, De Graeve et al., 2004, Isshiki et al., 1997, Schmidt et al., 1997, Doe, 1992, Bhat, 1998, Chu et al., 1998, Bossing et al., 1996, Broadus et al., 1995, Bhat, 1996, Matsuzaki and Saigo, 1996, Bhat and Schedl, 1997, Buescher and Chia, 1997, Baumgardt et al., 2007, Losada-Pérez et al., 2010, Beckervordersandforth et al., 2008, Lundell et al., 1996) hkb⁵⁹⁵³ (Bhat, 1996)

Lethality References viable (Andrew et al., 1994, Bhat, 1996)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage 9 -- 10 ventral neurogenic region (Chu-LaGraff and Doe, 1993) Comment:row 5 embryonic stage 10 -- 11 neuroblast NB2-2 (Doe, 1992) embryonic stage 11 neuroblast NB2-4 (Doe, 1992) neuroblast NB5-5 (Doe, 1992) Comment:late stage 11 neuroblast NB5-4 (Doe, 1992) distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage neuroblast of ventral nervous system \| subset (Higashijima et al., 1996) embryonic stage 9 -- 10 ventral neurogenic region (Chu-LaGraff and Doe, 1993) Comment:row 2/3 neuroblast NB2-3 (Chu-LaGraff and Doe, 1993) embryonic stage 9 -- 11 neuroblast NB2-3 (Doe, 1992) deuterocerebral neuroblast \| subset (Urbach and Technau, 2003) procephalic neurogenic region & embryonic antennal segment \| restricted (Urbach and Technau, 2003) Comment:stripe of expression tritocerebral neuroblast \| subset (Urbach and Technau, 2003) procephalic neurogenic region & embryonic ocular segment \| restricted (Urbach and Technau, 2003) protocerebral neuroblast \| subset (Urbach and Technau, 2003) procephalic neurogenic region & embryonic intercalary segment \| anterior & restricted (Urbach and Technau, 2003) embryonic stage 10 -- 11 neuroblast NB1-1 (Bhat et al., 2000) Comment:reference states 5-5.5 hr AEL neuroblast NB2-2 (Bhat et al., 2000) Comment:reference states 5-5.5 hr AEL neuroblast NB2-5 (Bhat et al., 2000) Comment:reference states 5-5.5 hr AEL neuroblast NB4-2 (Bhat et al., 2000) Comment:reference states 5-5.5 hr AEL embryonic stage 11 neuroblast NB4-4 (Doe, 1992) neuroblast NB7-3 (Doe, 1992) Comment:late stage 11 neuroblast NB4-3 (Doe, 1992) Comment:late stage 11 neuroblast NB2-1 (Doe, 1992) embryonic stage 17 A-subperineurial glial cell of abdomen \| faint (Bossing et al., 1996) A-subperineurial glial cell of thorax \| faint (Bossing et al., 1996) B-subperineurial glial cell of abdomen (Bossing et al., 1996) RP2 neuron (Bossing et al., 1996) ventro-lateral serotonergic neuron (Lundell et al., 1996)
Additional Information
Statement Reference Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d6, d8, v1; deuterocerebrum - d1-10, d12, d13, v1-v8; protocerebrum - cd2, cv7 (Urbach and Technau, 2003) Ecol\lacZ^hkb-5953 is expressed in the CNSin row 2/3 and in patches of cells in rows 4 and 5 in wild type embryos.In wg mutant embryos, there is selective loss of Ecol\lacZ^hkb-5953 expressionin row 4. (Chu-LaGraff and Doe, 1993) Enhancer trap expression is observed in a specified subset of neuroblasts in embryonic stages 9-11. (Doe, 1992)

Marker for	Dd1 neuroblast (Urbach and Technau, 2003) Dd2 neuroblast (Urbach and Technau, 2003) Dd3 neuroblast (Urbach and Technau, 2003) Dd4 neuroblast (Urbach and Technau, 2003) Dd5 neuroblast (Urbach and Technau, 2003) Dd6 neuroblast (Urbach and Technau, 2003) Dd7 neuroblast (Urbach and Technau, 2003) Dd8 neuroblast (Urbach and Technau, 2003) Dd9 neuroblast (Urbach and Technau, 2003) Dd10 neuroblast (Urbach and Technau, 2003) Dd12 neuroblast (Urbach and Technau, 2003) Dd13 neuroblast (Urbach and Technau, 2003) deuterocerebral neuroblast \| subset (Urbach and Technau, 2003) Dv1 neuroblast (Urbach and Technau, 2003) Dv2 neuroblast (Urbach and Technau, 2003) Dv3 neuroblast (Urbach and Technau, 2003) Dv4 neuroblast (Urbach and Technau, 2003) Dv5 neuroblast (Urbach and Technau, 2003) Dv6 neuroblast (Urbach and Technau, 2003) Dv7 neuroblast (Urbach and Technau, 2003) Dv8 neuroblast (Urbach and Technau, 2003) embryonic brain \| restricted (Urbach and Technau, 2003) neuroblast NB1-1 (Bhat et al., 2000) neuroblast NB2-2 (Bhat et al., 2000) neuroblast NB2-5 (Bhat et al., 2000) neuroblast NB4-2 (Bhat et al., 2000) neuroblasts of ventral nervous system \| subset (Higashijima et al., 1996) Pcd2 neuroblast (Urbach and Technau, 2003) Pcv7 neuroblast (Urbach and Technau, 2003) procephalic neuroblast \| subset (Urbach and Technau, 2003) protocerebral neuroblast \| subset (Urbach and Technau, 2003) Td6 neuroblast (Urbach and Technau, 2003) Td8 neuroblast (Urbach and Technau, 2003) tritocerebral neuroblast \| subset (Urbach and Technau, 2003) Tv1 neuroblast (Urbach and Technau, 2003)
Reflects expression of	hkb (Urbach and Technau, 2003)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	6947 w^*; P{lacZ-un4}hkb⁵⁹⁵³ ded^U9/TM3, Sb¹
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	5933 (Urbach and Technau, 2003) 5953 (Lundell et al., 1996) 5953-LacZ (Higashijima et al., 1996) hkb-lacZ (Urbach and Technau, 2003, Losada-Pérez et al., 2010) huckebein-lacZ (Urbach and Technau, 2003, De Graeve et al., 2004, Ragone et al., 2003, Losada-Pérez et al., 2010, Beckervordersandforth et al., 2008) line 5953 (Andrew et al., 1994) P{lacZ-un4}hkb⁵⁹⁵³ (FlyBase, 1992-, Baumgardt et al., 2007)
Secondary FlyBase IDs

References ( 28 )

Generate a list of	All references relating to this insertion ( 28 )

List References by type	Research paper ( 25 ) Review ( 2 ) FlyBase analysis ( 1 )
Recent research papers ( 1 )
	Losada-Pérez et al., 2010, Mech. Dev. 127(9-12): 458--471 Lineage-unrelated neurons generated in different temporal windows and expressing different combinatorial codes can converge in the activation of the same terminal differentiation gene. [FBrf0212045] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...

Dmel\P{lacZ-un4}hkb5953 Insertion

Dmel\P{lacZ-un4}hkb⁵⁹⁵³ Insertion