Dmel\P{PZ}Dscam⁰⁵⁵¹⁸ Insertion

General Information
Symbol	Dmel\P{PZ}Dscam⁰⁵⁵¹⁸	Species	D. melanogaster
Name		FlyBase ID	FBti0002607
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Dscam, Ecol\lacZ	Viability / fertility
Causes allele(s)	Dscam⁰⁵⁵¹⁸, Ecol\lacZ^Dscam-05518	Stock availability	1 publicly available
LINE ID	l(2)05518
Genomic Location
Chromosomal location	2R ( 43B1 )	Sequence location	2R:3,253,154..3,253,154 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:3,253,154..3,253,154 [+] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	43B1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	43B1-43B2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034165 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Dscam (Roote, 1995.9.26) Ecol\lacZ (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Jackle and Janning, 1998.8.10)
Alleles and Phenotypes
Causes alleles	Dscam⁰⁵⁵¹⁸ (Roote, 1995.9.26, Wang et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999, Schmucker et al., 2000, Chen et al., 2006) Ecol\lacZ^Dscam-05518 (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Jackle and Janning, 1998.8.10)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	Bolwig nerve (Andrews et al., 2008, Schmucker et al., 2000) commissure (Schmucker et al., 2000, Andrews et al., 2008) longitudinal connective (Schmucker et al., 2000)
Detailed Description
Statement Reference 27.5% of homozygous Dscam[05518] embryos show defects in axon guidance in the Bolwig\'s nerve. 30% of segments fail to separate the anterior and posterior commissures correctly in Dscam[05518] embryos. (Andrews et al., 2008) 67% of Dscam[05518]/Dscam[05518] fra[4]/fra[4] embryos show defects in axon guidance in the Bolwig\'s nerve. The penetrance of the axon guidance defect phenotype that is seen in the Bolwig\'s nerve of Dscam[05518]/Dscam[05518] fra[4]/fra[4] double mutant embryos is not increased by addition of Dscam3[c02826]/Dscam3[c02826]. 22.5% of Dscam[05518]/+ ; fra[4]/+ embryos show defects in axon guidance in the Bolwig\'s nerve. 38% of Df(1)NP5/+ ; Dscam[05518]/+ embryos show defects in axon guidance in the Bolwig\'s nerve. 34% of Df(1)NetAB[Δ]/+ ; Dscam[05518]/+ embryos show defects in axon guidance in the Bolwig\'s nerve. 84% of segments fail to separate the anterior and posterior commissures correctly in Dscam[05518] Dscam3[c02826] embryos. Dscam[05518] fra[4] embryos show defects in the commissures of the central nervous system; 9% of anterior commissures are absent, 24% of anterior commissures are thin, 5% of posterior commissures are absent and 39% of posterior commissures are thin. 51% of segments fail to separate the anterior and posterior commissures correctly. Dscam[05518] Abl[4] embryos show defects in the commissures of the central nervous system; 23% of anterior commissures are absent, 33% of anterior commissures are thin, 65% of posterior commissures are absent and 26% of posterior commissures are thin. 19% of segments fail to separate the anterior and posterior commissures correctly. Dscam[05518] fra[4] Dscam3[c02826] embryos show defects in the commissures of the central nervous system; 39% of anterior commissures are absent, 51% of anterior commissures are thin, 36% of posterior commissures are absent and 55% of posterior commissures are thin. 5% of segments fail to separate the anterior and posterior commissures correctly. Dscam[05518] fra[4] Abl[4] embryos show defects in the commissures of the central nervous system; 98% of anterior commissures are absent, 2% of anterior commissures are thin and 100% of posterior commissures are absent. The severity of the SP1 axon midline crossing defects seen in fra[unspecified] embryos is enhanced in fra[unspecified] Dscam[05518] Dscam3[c02826] triple mutants. 37% of EG axon bundles are thin or absent in Dscam[05518] fra[unspecified] mutant embryos. (Andrews et al., 2008) About 42% of Dscam⁰⁵⁵¹⁸/dock³ embryos exhibit some Bolwig\'s nerve targeting defects. About 38% of Dscam⁰⁵⁵¹⁸/Pak⁶ embryos exhibit some Bolwig\'s nerve targeting defects. (Schmucker et al., 2000) About 5% of heterozygotes exhibit some Bolwig\'s nerve targeting defects. Dscam⁰⁵⁵¹⁸ hemizygous mutants exhibit mild to severe disorganisation of embryonic axon tracts. Breaks in the connectives, predominantly of the outer two fascicles are observed. In addition axon bundles aberrantly cross the midline. Some 53%-58% of Bolwig\'s nerve (BN) projections are defective in Dscam¹/Df(2R)cos-2 hemizygotes. In half of the "abnormal projections" the entire nerve mistargets, whereas in the remainder only a subset of axons does. Many otherwise normal projections show abnormal expansion of BN terminus at P2. These phenotypes are 53-58% penetrant. (Schmucker et al., 2000)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage \| early cephalic furrow \| anterior to (Jackle and Janning, 1998.8.10) embryonic stage organism \| segmentally repeated (Jackle and Janning, 1998.8.10) presumptive embryonic/larval peripheral nervous system (Jackle and Janning, 1998.8.10) anterior embryonic/larval midgut (Jackle and Janning, 1998.8.10) embryonic/larval brain \| presumptive (Jackle and Janning, 1998.8.10) posterior embryonic/larval midgut (Jackle and Janning, 1998.8.10) epidermis (Jackle and Janning, 1998.8.10) imaginal disc primordium (Jackle and Janning, 1998.8.10) embryonic/larval pharynx (Jackle and Janning, 1998.8.10) presumptive embryonic/larval central nervous system \| restricted (Jackle and Janning, 1998.8.10)
Additional Information
Statement Reference In early embryo, stripe of expression anterior to cephalic furrow. (Jackle and Janning, 1998.8.10) In wandering third instar larva, no detectable β-gal staining. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)05518 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11412 P{PZ}Dscam⁰⁵⁵¹⁸ cn¹/CyO; ry⁵⁰⁶
Linkouts
Comments
	Location 2R:2877461-2877462 determined by FlyBase alignment of dbGSS accession AQ034165 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1412 (Meister and Braun, 1995.10) P{PZ}Dscam⁰⁵⁵¹⁸ (FlyBase, 2005, FlyBase, 1992-) P{PZ}l(2)43Bc⁰⁵⁵¹⁸ (FlyBase, 1992-) P{PZ}l(2)05518⁰⁵⁵¹⁸ (FlyBase, 1992-) P{ry^+t7.2=PZ}l(2)43Bc⁰⁵⁵¹⁸ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000448
References ( 15 )
Research paper	Andrews et al., 2008, Development 135(23): 3839--3848 Dscam guides embryonic axons by Netrin-dependent and -independent functions. [FBrf0206236] Chen et al., 2006, Cell 125(3): 607--620 The molecular diversity of Dscam is functionally required for neuronal wiring specificity in Drosophila. [FBrf0189904] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Wang et al., 2002, Neuron 33(4): 559--571 Drosophila dscam is required for divergent segregation of sister branches and suppresses ectopic bifurcation of axons. [FBrf0145178] Schmucker et al., 2000, Cell 101(6): 671--684 Drosophila Dscam is an axon guidance receptor exhibiting extraordinary molecular diversity. [FBrf0128634] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Jackle and Janning, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103143] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] Roote, 1995.9.26, 43 lethals. 43 lethals. [FBrf0086245]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}Dscam05518 Insertion

Dmel\P{PZ}Dscam⁰⁵⁵¹⁸ Insertion