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General Information
Symbol
Dmel\P{GawB}E132
Species
D. melanogaster
Name
FlyBase ID
FBti0002638
Also Known As
upd-gal4, E132-Gal4, upd1-gal4
Description
Inserted Element
Uses

GAL4 reporter/driver

Affected gene(s)
Viability / fertility
viable
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
X ( 17A5 )
Sequence Location
X:18,312,700..18,312,700
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:18,312,700..18,312,700
Orientation
Cytological location (computed by FlyBase)
17A5 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location

The inseriton site is reported to be 2851 bp upstream of cDNA GB:AF231684.

Sequence Data
Flanking sequence
Inserted Element
Construct
Location-dependent role
Size (Kb)

11.279

Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
electrophoresis
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in the distal-most part of the wing disc; in a lateral stripe in the outer optic anlage and in the lamina furrow in the eye disc; in polar follicle cells during oogenesis and in hub cells during spermatogenesis; and in DN period neurons in the adult brain. Also can be used as a read-out for activation of the JAK/STAT pathway.

At 48 hours, upd1 reporter is expressed broadly in the presumptive pouch, hinge and the lateral part of the notum. It is excluded from the medial notum and the peripodial epithelium. At 60 hr, expression is down-regulated in the distal pouch and notum. At 96-120 hr, expression is restricted to the lateral border of the notum. bowl and upd1 reporter are expressed in adjacent domains along the anterior margin in an interlocking pattern.

Scer\GAL4upd1-E132 drives expression in small-nucleated progenitors to enterocytes (e.g., intestinal stem cells and enteroblasts).

Scer\GAL4upd1-E132 drives expression in a widespread pattern in the adult brain, including the DN clock neurons and some lateral located neurons. Dendrites of both of these neuron clusters are located near the projections of the PDF positive sLNV clock neurons.

upd1-GAL4-expressing neurons comprise a subset of mushroom body alph/beta and gamma neurons. These neurons are organized into four distinct bundles, visible within the mushroom body peduncle. The four upd1 axon tracts initally enter the peduncle separately, but fuse into two final bundles at a more anterior level. upd1-expressing neurons represent a subset of alpha/beta and gamma neurons.

Scer\GAL4upd1-E132 drives expression in a stripe of cells along the most-lateral edge of the larval outer optic anlage and then becomes localized in the lamina furrow after the furrow forms around mid-third instar.

In second instar larva, expression throughout much of the ventral region of the eye-antennal disc, with staining expanding posteriorly to cover the entire dorsoventral extent of the eye disc. In third instar larva, expression in the antennal disc and in a small patch of cells at the posterior of the eye disc near the optic stalk.

 
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (19)
References (44)