A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{hsneo}stg14 Insertion

General Information
Symbol Dmel\P{hsneo}stg14 Species D. melanogaster
Name FlyBase ID FBti0002679
Feature type transposable_element_insertion_site
Description
Inserted element P{hsneo} Expression data
Affected gene(s) stg Viability / fertility
Causes allele(s) stg14 Stock availability none publicly available
LINE ID l(3)neo62
Genomic Location
Chromosomal location 3R ( 99A5 ) Sequence location
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{hsneo}; most lines have a lethal or sterile phenotype. The P{hsneo} construct carries a neomycin-resistance gene with a heat-shock promoter (allowing chemical selection in flies using G418), and bacterial sequences that allow plasmid rescue. First dataset of this type.
Insertion lines from this collection were assessed for inclusion in the BDGP Gene Disruption Project collection.
(Cooley et al., 1988, Spradling et al., 1999)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
99A5 ( near gene of known cytology )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{hsneo}
Location-dependent
role
Size 5.075Kb
Associated alleles
Tn\neoRHsp70Bb.PS
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
stg
(Edgar and O'Farrell, 1989)
hide Alleles and Phenotypes
Causes alleles
stg14
(Edgar and O'Farrell, 1989, Lindsley and Zimm, 1992, Berg and Spradling, 1991, Cooley et al., 1988)
Lethality
References
lethal | embryonic stage | recessive
(Edgar and O'Farrell, 1989)
lethal | recessive
(Lindsley and Zimm, 1992, Cooley et al., 1988)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Does not display a stg phenotype.
(Lindsley and Zimm, 1992)
fails to complement completely strong stg alleles.
(Lindsley and Zimm, 1992)
stg14 fails to complement stg5, stg3 and stg2, and only partially complements stg8, stg6, stg4, stg1 and stg7. stg14 homozygotes do not show a stg mutant cuticle phenotype. stg3/stg14 transheterozygotes show a weak stg mutant cuticle phenotype.
(Edgar and O'Farrell, 1989)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)neo62
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{hsneo}stg14
(FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Berg and Spradling, 1991, Genetics 127: 515--524
Studies on the rate and site-specificity of P-element transposition. [FBrf0054167]
Edgar and O'Farrell, 1989, Cell 57: 177--187
Genetic control of cell division patterns in the Drosophila embryo. [FBrf0049340]
Cooley et al., 1988, Science 239: 1121--1128
Insertional mutagenesis of the Drosophila genome with single P-elements. [FBrf0049003]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]
Book
Lindsley and Zimm, 1992, The Genome of Drosophila melanogaster.
The Genome of Drosophila melanogaster. [FBrf0066905]