Dmel\P{PZ}wg^rO727 Insertion

General Information
Symbol	Dmel\P{PZ}wg^rO727	Species	D. melanogaster
Name		FlyBase ID	FBti0002727
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, wg	Viability / fertility
Causes allele(s)	Ecol\lacZ^wg-rO727, wg^rO727	Stock availability	none publicly available
LINE ID	l(2)rO727
Genomic Location
Chromosomal location	2L ( 27F1 )	Sequence location	2L:7,307,139..7,307,139 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:7,307,139..7,307,139 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	27F1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	28F1-28F2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026139 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Neumann and Cohen, 1996, Diaz-Benjumea and Cohen, 1994, Kopp et al., 1999, Kopp et al., 1997, Neumann and Cohen, 1998, Majumdar et al., 1997, Akimaru et al., 1997) wg (Diaz-Benjumea et al., 1994, Neumann and Cohen, 1996)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^wg-rO727 (Neumann and Cohen, 1996, Diaz-Benjumea and Cohen, 1994, Kopp et al., 1999, Kopp et al., 1997, Neumann and Cohen, 1998, Majumdar et al., 1997, Akimaru et al., 1997) wg^rO727 (Diaz-Benjumea et al., 1994, Neumann and Cohen, 1996, BDGP Project Members, 1994-1999, Neumann and Cohen, 1996)

Lethality References lethal \| embryonic stage \| recessive (Neumann and Cohen, 1996) lethal \| embryonic stage \| recessive \| segment polarity expression pattern (Neumann and Cohen, 1996)
Sterility References
Phenotype Manifest In
	embryo \| segment polarity expression pattern (Neumann and Cohen, 1996) embryonic abdominal segment (Neumann and Cohen, 1996)
Detailed Description
Statement Reference Weak segment polarity phenotype. (Neumann and Cohen, 1996) Weak embryonic segmentation defect. (Neumann and Cohen, 1996)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage imaginal disc \| restricted (Meister and Braun, 1995.10) embryonic/larval hindgut (Meister and Braun, 1995.10)
Additional Information
Statement Reference In third instar larva, high level of expression in imaginal discs (which discs not specified), lower level in hindgut. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)rO727 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 2L:7307139-7307140 confirmed by FlyBase alignment of dbGSS accession AQ026139 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005) This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}l(2)rO727^rO727 (FlyBase, 1992-) P{PZ}wg^rO727 (FlyBase, 2005, FlyBase, 1992-, ) P{ry^+t7.2=PZ}wg^rO727 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000753
References ( 19 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Giraldez et al., 2002, Dev. Cell 2(5): 667--676 HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient. [FBrf0148946] Kopp et al., 1999, Development 126(16): 3495--3507 Wingless, decapentaplegic and EGF receptor signaling pathways interact to specify dorso-ventral pattern in the adult abdomen of Drosophila. [FBrf0110096] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Neumann and Cohen, 1998, Science 281(5375): 409--413 Boundary formation in Drosophila wing: Notch activity attenuated by the POU protein Nubbin. [FBrf0103338] Akimaru et al., 1997, Nature 386(6626): 735--738 Drosophila CBP is a co-activator of cubitus interruptus in hedgehog signalling. [FBrf0093058] Kopp et al., 1997, Development 124(19): 3703--3714 The roles of hedgehog and engrailed in patterning adult abdominal segments of Drosophila. [FBrf0099018] Majumdar et al., 1997, Genes Dev. 11(10): 1341--1353 strawberry notch encodes a conserved nuclear protein that functions downstream of Notch and regulates gene expression along the developing wing margin of Drosophila. [FBrf0093584] Neumann and Cohen, 1996, Genetics 142(4): 1147--1155 Sternopleural is a regulatory mutation of wingless with both dominant and recessive effects on larval development of Drosophila melanogaster. [FBrf0087628] Neumann and Cohen, 1996, Development 122(6): 1781--1789 Distinct mitogenic and cell fate specification functions of wingless in different regions of the wing. [FBrf0088656] Diaz-Benjumea et al., 1994, Nature 372(6502): 175--179 Cell interaction between compartments establishes the proximal-distal axis of Drosophila legs. [FBrf0076987] Diaz-Benjumea and Cohen, 1994, Development 120(6): 1661--1670 wingless acts through the shaggyzeste-white 3 kinase to direct dorsal-ventral axis formation in the Drosophila leg. [FBrf0072960] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}wgrO727 Insertion

Dmel\P{PZ}wg^rO727 Insertion