FlyBase Insertion Report: Dmel\P{GawB}Dll[md23]

General Information

Symbol

Dmel\P{GawB}Dll^md23

Species

D. melanogaster

Name

FlyBase ID

FBti0002783

Feature type

transposable_element_insertion_site

Also Known As

Dll-GAL4, DllGal4, dll^md23-Gal4, Dll-GAL4^md23, Dll-GAL4 (MD23), dll.Gal4, P[Dll-Gal4], Dll^md23, dll, MD23

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Dll, Scer\GAL4

Viability / fertility

fertile, viable

Associated allele(s)

Dll^md23, Scer\GAL4^Dll-md23

Stock availability

3 publicly available

Genomic Location

Chromosomal Location

2R ( 60E2 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

60E2 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Dll

(Dey et al., 2009, Calleja, 1996.10.16)

Scer\GAL4

(Papadopoulos et al., 2012, Plavicki et al., 2012, Giorgianni and Mann, 2011, Gradilla et al., 2011, Morante et al., 2011, O'Keefe et al., 2011, Ouyang et al., 2011, Pueyo and Couso, 2011, Heffer et al., 2010, Nagai et al., 2010, Papadopoulos et al., 2010, Papadopoulos et al., 2010, Rendina et al., 2010, Umehara et al., 2010, Greenberg and Hatini, 2009, Loya et al., 2009, Terriente-Félix and de Celis, 2009, Brás-Pereira and Casares, 2008, Hamada et al., 2008, Morante and Desplan, 2008, Villányi et al., 2008, Barrio et al., 2007, Félix et al., 2007, Galindo et al., 2007, Garg et al., 2007, Sakurai et al., 2007, Stevens and Mann, 2007, Akasaka et al., 2006, Baena-Lopez and Garcia-Bellido, 2006, Matakatsu and Blair, 2006, Morris et al., 2006, Venkei et al., 2006, Akimoto et al., 2005, Lohr, 2005, Lohr and Pick, 2005, Takaesu et al., 2005, Hamaguchi et al., 2004, Matakatsu and Blair, 2004, Otsuki et al., 2004, Pueyo and Couso, 2004, Sakata, 2004, Yoshida et al., 2004, Astle et al., 2003, Baena-Lopez and Garcia-Bellido, 2003, de Celis Ibeas and Bray, 2003, Emerald et al., 2003, Enerly et al., 2003, Fortier et al., 2003, Hao et al., 2003, Kenyon et al., 2003, Kondoh et al., 2003, Kubota et al., 2003, Roignant et al., 2003, Suzanne et al., 2003, Ward et al., 2003, Azpiazu and Morata, 2002, Dong et al., 2002, Enerly et al., 2002, Galindo et al., 2002, Galindo et al., 2002, Held, 2002, Lee and Adler, 2002, Lockwood and Bodmer, 2002, Nakamura et al., 2002, Reichhart et al., 2002, Shiga et al., 2002, Veraksa et al., 2002, Kammermeier et al., 2001, Lohr et al., 2001, Marquez et al., 2001, Mishra et al., 2001, True et al., 2001, Yoshida et al., 2001, Boube et al., 2000, Boutros et al., 2000, Cavodeassi et al., 2000, Collins and Treisman, 2000, Kubota et al., 2000, Mukherjee et al., 2000, Ruiz-Gomez et al., 2000, Takatsu et al., 2000, Bishop et al., 1999, Gorfinkiel et al., 1999, Moreno and Morata, 1999, Ryoo et al., 1999, True et al., 1999, Weigmann and Cohen, 1999, Casares and Mann, 1998, Halder et al., 1998, Weatherbee et al., 1998, Adler et al., 1997, Gorfinkiel et al., 1997, Lecuit and Cohen, 1997, Calleja, 1996.10.16, Calleja et al., 1996, Gonzalez-Crespo and Morata, 1996)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Dll^md23

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^Dll-md23

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

head | restricted

(Calleja et al., 1996)

thorax | restricted

(Calleja et al., 1996)

pupal stage & adult stage

leg | restricted

(Calleja et al., 1996)

antenna | restricted

(Calleja et al., 1996)

wing | restricted

(Calleja et al., 1996)

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage

larval outer optic anlage | restricted

(Morante et al., 2011)

adult stage

cell body rind of adult medulla

(Morante et al., 2011)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10 -- 17

embryonic head | restricted

(Marquez et al., 2001)

embryonic stage 11

embryonic head segment

(Calleja et al., 1996)

embryonic thoracic segment

(Calleja et al., 1996)

embryonic stage 12 -- 14

dorsal thoracic disc primordium

(Inoue and Hayashi, 2007)

prothoracic segment | restricted

(Inoue and Hayashi, 2007)

metathoracic segment | restricted

(Inoue and Hayashi, 2007)

larval stage

antennal disc | restricted

(Mukherjee et al., 2000)

leg disc | restricted

(Mukherjee et al., 2000)

third instar larval stage

male genital disc | restricted

(Gorfinkiel et al., 1999)

female genital disc | restricted

(Gorfinkiel et al., 1999)

wing disc | restricted

(Marquez et al., 2001)

larval outer optic anlage | restricted

(Morante et al., 2011)

adult stage

femur

(Gorfinkiel et al., 1997)

arista

(Gorfinkiel et al., 1997)

tarsal segment

(Gorfinkiel et al., 1997)

leg | distal

(Gorfinkiel et al., 1997)

surstylus

(Gorfinkiel et al., 1999)

wing vein | distal

(Gorfinkiel et al., 1997)

wing margin

(Gorfinkiel et al., 1997)

antenna | distal

(Gorfinkiel et al., 1997)

trochanter

(Gorfinkiel et al., 1997)

hypogynium

(Gorfinkiel et al., 1999)

wing | distal

(Gorfinkiel et al., 1997)

second segment of antenna

(Gorfinkiel et al., 1997)

alar lobe

(Gorfinkiel et al., 1997)

third segment of antenna

(Gorfinkiel et al., 1997)

cercus

(Gorfinkiel et al., 1999)

tibia

(Gorfinkiel et al., 1997)

adult optic lobe | restricted

(Morante and Desplan, 2008)

medullary intrinsic neuron Mi5

(Morante and Desplan, 2008)

medullary intrinsic neuron Mi12

(Morante and Desplan, 2008)

distal medullary amacrine neuron Dm2

(Morante and Desplan, 2008)

distal medullary amacrine neuron Dm4

(Morante and Desplan, 2008)

distal medullary amacrine neuron Dm5

(Morante and Desplan, 2008)

distal medullary amacrine neuron Dm8

(Morante and Desplan, 2008)

centrifugal neuron C3

(Morante and Desplan, 2008)

Additional Information

Statement

Reference

Drives expression in presumptive distal structures in imaginal discs, including the presumptive tarsal segments of leg discs, the presumptive wing margin, the presumptive second and third segments and arista of the antennal disc, and distal structures of male and female genital discs.

(FlyBase Curators, 2017)

Comparing ScerGAL4^Dll-md23 with anti-Dll staining shows that the Gal4 driver faithfully recapitulates Dll expression with two exceptions: (1) the embryonic ventral nerve cord glia that express Dll lack ScerGAL4^Dll-md23 expression; and (2) several cells in thebrain that express Dll, also lack ScerGAL4^Dll-md23 expression.

(Plavicki et al., 2016)

Dll reporter is expressed in a small population of neurons located at the most proximal part of the outer optic lobe (neurons generated by the oldest neuroblasts to have emerged from the neuroepithelium) but not in the neuroblasts themselves. Expression persists in pupae.

(Morante et al., 2011)

ScerGAL4^Dll-md23 drives expression in 20% of cells in the medulla cortex and medulla rim. Neurons in the medulla rim have processes in the lamina. Cells labeled with ScerGAL4^Dll-md23 do not overlap with cells expressing EcollacZ^ap-rK568 or ScerGAL4^ey-OK107.

(Morante and Desplan, 2008)

Reporter expression was used to trace the movement of wing primordial cells in the embryo. In embryonic stages 12-14, expressing cell clusters in each thoracic segment extend in the dorsoventral direction. By stage 15, wing and haltere cells are well separated from leg primordia. A dorsal cluster of expressing cells is also observed in the prothoracic segment.

(Inoue and Hayashi, 2007)

Larval expression is observed in the genital and anal primordia of the male and female genital discs. Adult expression is observed in the vaginal plates in females, in the claspers in males, and in the anal plates of both sexes.

(Gorfinkiel et al., 1999)

Assayed in adult cuticle using y⁺ marker. Expressed in distal portions of leg, wing, and antenna; expression in femur relatively weak.

(Gorfinkiel et al., 1997)

Expression in distal portions of leg and antenna; along dorsal/ventral boundary of wing. Observed as adult y⁺ phenotype; expression assumed to be earlier.

(Calleja et al., 1996)

Marker for

cercus

(Gorfinkiel et al., 1999)

female genital disc

(Gorfinkiel et al., 1999)

hypogynium

(Gorfinkiel et al., 1999)

male genital disc

(Gorfinkiel et al., 1999)

surstylus

(Gorfinkiel et al., 1999)

Reflects expression of

Dll

(Morante and Desplan, 2008, Inoue and Hayashi, 2007, Marquez et al., 2001, Mukherjee et al., 2000, Gorfinkiel et al., 1999, Gorfinkiel et al., 1997, Calleja et al., 1996)

Reporter construct used in assay

Syt1^UAS.Tag:HA

(Morante and Desplan, 2008)

Dscam1^{exon17.1.UAS.GFP}

(Morante and Desplan, 2008)

Ecol\lacZ^{UAS.O.Tag:MT(btau)}

(Morante and Desplan, 2008)

Mmus\Cd8a^UAS.cLa.EGFP

(Morante and Desplan, 2008)

Ecol\lacZ^UAS.cBa

(Marquez et al., 2001, Calleja et al., 1996)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (3)

Bloomington

3038

P{GawB}Dll^md23/CyO

64307

y¹ w^67c23; P{UAS-mCD8::GFP.L}LL5 P{GawB}Dll^md23, P{UAS-mCD8::GFP.L}2/CyO, P{ActGFP}JMR1

Kyoto

106795

P{GawB}Dll^md23/CyO

External Crossreferences and Linkouts ( 0 )

Comments

Isolated using P{UAS-y.C} adult cuticle patterning screen; see FBrf0090484.

(Calleja, 1996.10.16)

Synonyms and Secondary IDs (43)

Reported As

Symbol Synonym

Distal-lesGal4-MD23

(Gradilla et al., 2011)

Dll

(Umehara et al., 2010)

Dll-G4

(Baena-Lopez and Garcia-Bellido, 2003)

Dll-GAL4 (MD23)

(Suzanne et al., 2003)

Dll-GAL4^md23

(de Madrid et al., 2015, Greenberg and Hatini, 2009)

Dll-Gal4 (MD23)

(Papadopoulos et al., 2012)

Dll-Gal4 (line MD 23)

(McKay et al., 2009)

Dll-Gal4(MD23)

(Papadopoulos et al., 2010)

Dll-Gal4^MD23

(Guarner et al., 2014)

Dll-gal4

(Akimoto et al., 2005, Kenyon et al., 2003)

Dll::Gal4^MD23

(Ambrosini et al., 2019)

Dll:Gal4

(Ryoo et al., 1999)

Dll^Gal4

(Emerald et al., 2003)

Dll^MD23-Gal4

(Requena et al., 2017)

Dll^md23

(Sakurai et al., 2007, Akimoto et al., 2005)

DllG4

(Baena-Lopez and Garcia-Bellido, 2006)

DllGAL4

(Grubbs et al., 2013, Hao et al., 2003, Dong et al., 2002)

DllGal4

(Garg et al., 2007, Emerald and Cohen, 2004, Pueyo and Couso, 2004, Galindo et al., 2002, Galindo et al., 2002, Boutros et al., 2000, Weigmann and Cohen, 1999)

DllGal4^MD23

(Félix et al., 2007)

GAL4-dll⁺

(Enerly et al., 2002)

Gal4-dll

(Barrio et al., 2007)

MD23

(Papadopoulos et al., 2019, González and Busturia, 2009)

MD23-GAL4

(Gorfinkiel et al., 1999)

P[Dll-Gal4]

(Kester and Nambu, 2011, Mukherjee et al., 2000)

P[GawB]Dll(m23)

(Kester and Nambu, 2011)

P{GawB}Dll^md23

(Kuzin et al., 2018, Akishina et al., 2017, Plavicki et al., 2016, Plavicki et al., 2012, Kester and Nambu, 2011, Brás-Pereira and Casares, 2008, FlyBase, 1992-)

P{w⁺mW.hs = GawB}Dll^md23

(Fortier et al., 2003)

P{w^+mW.hs=GawB}Dll.MD23

(Reichhart et al., 2002)

P{w^+mW.hs=GawB}Dll^md23

(FlyBase, 1992-)

distal less-Gal4

(González and Busturia, 2009)

distal-less-GAL4

(Roignant et al., 2003)

distalless Gal4

(Venkei et al., 2006)

distalless-G4

(Baena-Lopez and Garcia-Bellido, 2006)

dll

(Morris et al., 2006)

dll-GAL4

(Cho et al., 2020, Plavicki et al., 2012, Rendina et al., 2010, Hamaguchi et al., 2004, Otsuki et al., 2004, Lee and Adler, 2002, Yoshida et al., 2001)

dll-Gal4

(Suzuki et al., 2018, Hevia et al., 2017, Molnar and de Celis, 2013, González and Busturia, 2009, Terriente-Félix and de Celis, 2009, Morante and Desplan, 2008, Villányi et al., 2008, Barrio et al., 2007, Akasaka et al., 2006)

dll-gal4

(Matakatsu and Blair, 2006, Matakatsu and Blair, 2004)

dll.Gal4

(Takaesu et al., 2005, Marquez et al., 2001)

dll^md23-GAL4

(Caipo et al., 2020)

dll^md23-Gal4

(Morante et al., 2011, Morante and Desplan, 2008)

dllG4^md23

(Baena-Lopez and Garcia-Bellido, 2006)

Name Synonyms

Secondary FlyBase IDs

FBti0002813

References (127)

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