FlyBase Insertion Report: Dmel\P{GawB}tsh[md621]

General Information

Symbol

Dmel\P{GawB}tsh^md621

Species

D. melanogaster

Name

FlyBase ID

FBti0002787

Feature type

transposable_element_insertion_site

Also Known As

tsh-Gal4, tshGal4, teashirt-GAL4, tsh^md621-Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4, tsh

Viability / fertility

viable

Associated allele(s)

Scer\GAL4^tsh-md621, tsh^md621

Stock availability

3 publicly available

Genomic Location

Chromosomal Location

2L ( 40A5 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

40A5 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^tsh-md621

Phenotypic Class Phenotype Manifest in Detailed Description

tsh^md621

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage | male

larval abdominal segment 8 of male genital disc

(Petzoldt et al., 2012)

early third instar larval stage -- prepupal stage P4

abdominal segment 8 of male genital disc

(Speder et al., 2006)

wandering third instar larval stage

wing hinge primordium

(Peterson and O'Connor, 2013, Pallavi and Shashidhara, 2003)

mesothoracic tergum primordium

(Peterson and O'Connor, 2013, Pallavi and Shashidhara, 2003)

peripodial epithelium | restricted of wing disc

(Pallavi and Shashidhara, 2003)

adult stage

adult fruitless aSP-g (female) lineage clone

(Yu et al., 2010)

peripodial epithelium | restricted of wing disc | restricted

(Gibson et al., 2002)

Additional Information

Statement

Reference

Drives wing disc expression in the wing hinge primordium and the mesothoracic tergum primordium (presumptive notum). Also drives expression in a subset of olfactory sensory and projection neurons.

(FlyBase Curators, 2017)

ScerGAL4^tsh-md621 drives expression in the wing hinge primordium and in the mesothoracic tergum primordium (presumptive notum).

(Peterson and O'Connor, 2013)

ScerGAL4^tsh-md621 drives expression in the region of the male genital disc corresponding to abdominal segment 8.

(Petzoldt et al., 2012)

ScerGAL4^tsh-md621 drives expression in the A8 segment of the male genital disc.

(Speder et al., 2006)

ScerGAL4^tsh-md621 drives expression in the wing hinge primordium and in the mesothoracic tergum primordium (presumptive notum; expression is driven in both the disc proper, and in the peripodial epithelium.

(Pallavi and Shashidhara, 2003)

ScerGAL4^tsh-md621 is expressed in peripodial cells of the wing disc, but is repressed in columnar epithelial cells of the presumptive wing blade.

(Gibson et al., 2002)

Expression in imaginal discs and embryos consistent with that of the tsh gene.

(Calleja, 1996.10.16)

Marker for

Reflects expression of

tsh

(Peterson and O'Connor, 2013, Petzoldt et al., 2012, Yu et al., 2010, Speder et al., 2006, Pallavi and Shashidhara, 2003, Calleja, 1996.10.16)

Reporter construct used in assay

Mmus\Cd8a^{FRT.UAS.cYa.GFP}

(Yu et al., 2010)

Disc\RFP^{UAS.Tag:Myr(Src64B)}

(Speder et al., 2006)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (3)

Bloomington

3040

y¹ w¹¹¹⁸; P{GawB}tsh^md621/CyO; P{UAS-y.C}MC1/TM2

90932

w^*; P{GawB}tsh^md621 P{UAS-FLP.D}JD1; P{Ubi-GFP.D}61EF P{neoFRT}80B/T(2;3)TSTL14, SM5: TM6B, Tb¹

Kyoto

106797

y¹ w¹¹¹⁸; P{GawB}tsh^md621/CyO; P{UAS-y.C}MC1/TM2

External Crossreferences and Linkouts ( 0 )

Comments

Isolated using P{UAS-y.C} adult cuticle patterning screen; see FBrf0090484.

(Calleja, 1996.10.16)

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

GAL4-tsh⁺

(Enerly et al., 2002)

P{GawB}md621

(FlyBase, 1992-)

P{GawB}tsh^md621

(FlyBase, 1992-)

P{w^+mW.hs=GawB}md621

(FlyBase, 1992-)

P{w[+mW.hs] = GawB}tsh[md621]

(Fedotov et al., 2014)

Tsh-GAL4

(Wang et al., 2013)

Tsh-GAL4^md621

(Hatini et al., 2013)

teashirt-GAL4

(Guichard et al., 2006)

teashirt-Gal4

(Paul et al., 2013, Zhang et al., 2013)

tsh

(Morris et al., 2006)

tsh-GAL4

(Wong et al., 2023, Zhou et al., 2016, Fedotov et al., 2014, Paul et al., 2013, Peterson and O'Connor, 2013)

tsh-Gal4

(Hérault et al., 2024, Botero et al., 2021, Chougule et al., 2020, King et al., 2020, Wang and Spradling, 2020, Dardalhon-Cuménal et al., 2018, Pathak et al., 2013, Nakajima et al., 2011, McKay et al., 2009, Zirin and Mann, 2007, Bose et al., 2006, Speder et al., 2006, Gorfinkiel et al., 2003, Villa-Cuesta et al., 2003, Cavodeassi et al., 2002)

tsh::Gal4

(Petzoldt et al., 2012)

tsh^md621-GAL4

(Zhou et al., 2016)

tsh^md621-Gal4

(Chougule et al., 2020)

tshG4

(Paul et al., 2013)

tshGAL4

(Soller et al., 2006)

tshGal4

(Resino and Garcia-Bellido, 2004, Weigmann and Cohen, 1999)

tshirt-GAL4

(Yu et al., 2010)

Name Synonyms

Secondary FlyBase IDs

FBti0003053

References (40)

Report Sections

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