Dmel\P{GawB}30Y Insertion

General Information
Symbol	Dmel\P{GawB}30Y	Species	D. melanogaster
Name		FlyBase ID	FBti0002931
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GawB}	Expression data	GAL4 reporter/driver
Affected gene(s)	Scer\GAL4	Viability / fertility
Causes allele(s)	Scer\GAL4^30Y	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3L ( 70E )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms core layer of the larval mushroom body outer layer of the larval mushroom body alpha'/beta' Kenyon cell inner layer of the larval mushroom body alpha/beta Kenyon cell surface layer of the larval mushroom body gamma Kenyon cell
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)	70E (in situ hybridization reported) (Tettamanti et al., 1997) 70E (in situ hybridization reported) (Yang, 1997.3.14) 70E (in situ hybridization reported) (Yang et al., 1995)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{GawB}
Location-dependent role	GAL4 driver/enhancer trap (Brand and Perrimon, 1993)
Size	11.279Kb
Associated alleles	Scer\GAL4^wB w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	Scer\GAL4 (Bushey et al., 2009, Yang et al., 1995, McBride et al., 1999, Belgacem and Martin, 2002, Gatti et al., 2000, Joiner and Griffith, 2000, Zars et al., 2000, Zars et al., 2000, Tettamanti et al., 1997, Rosay et al., 2001, Kraft et al., 1998, McGuire et al., 2001, Connolly et al., 1996, Amrein and Axel, 1997, Yang, 1997.3.14, Pitman et al., 2006, Joiner et al., 2006, Mehren and Griffith, 2004, Shafer et al., 2008, Stavropoulos and Young, 2011, Tzortzopoulos and Skoulakis, 2007, Donlea et al., 2009, Mehren and Griffith, 2006, Armstrong et al., 1998, Khurana et al., 2006, Ghosh and Feany, 2004, Carhan et al., 2005, Crocker et al., 2010, Aso et al., 2009, Pfeiffenberger and Allada, 2012, Pauls et al., 2010, Sakai and Kitamoto, 2006)
Alleles and Phenotypes
Causes alleles	Scer\GAL4^30Y (Yang et al., 1995, McBride et al., 1999, Belgacem and Martin, 2002, Bushey et al., 2009, Gatti et al., 2000, Joiner and Griffith, 2000, Zars et al., 2000, Zars et al., 2000, Tettamanti et al., 1997, Rosay et al., 2001, Kraft et al., 1998, McGuire et al., 2001, Connolly et al., 1996, Amrein and Axel, 1997, Yang, 1997.3.14, Pitman et al., 2006, Joiner et al., 2006, Mehren and Griffith, 2004, Shafer et al., 2008, Stavropoulos and Young, 2011, Tzortzopoulos and Skoulakis, 2007, Donlea et al., 2009, Mehren and Griffith, 2006, Armstrong et al., 1998, Khurana et al., 2006, Ghosh and Feany, 2004, Carhan et al., 2005, Crocker et al., 2010, Aso et al., 2009, Pfeiffenberger and Allada, 2012, Pauls et al., 2010, Sakai and Kitamoto, 2006)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference adult stage adult mushroom body (Yang et al., 1995) distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference larval stage mushroom body calyx (Tettamanti et al., 1997) Kenyon cell \| subset (Tettamanti et al., 1997) second instar larval stage -- third instar larval stage mushroom body alpha'-lobe \| restricted (Tettamanti et al., 1997) mushroom body alpha-lobe \| restricted (Tettamanti et al., 1997) mushroom body pedunculus (Tettamanti et al., 1997) third instar larval stage embryonic/larval mushroom body (Armstrong et al., 1998) pupal stage P5 mushroom body alpha-lobe (Armstrong et al., 1998) pupal stage P5 -- P7 mushroom body (Armstrong et al., 1998) mushroom body beta-lobe (Armstrong et al., 1998) pupal stage P6 mushroom body alpha-lobe \| restricted (Armstrong et al., 1998) pupal stage P6 -- P7 mushroom body gamma-lobe (Armstrong et al., 1998) mushroom body alpha'-lobe (Armstrong et al., 1998) Comment:Authors refer to the alpha'-lobe as the 'delta lobe'. pupal stage P7 mushroom body alpha-lobe (Armstrong et al., 1998) adult stage lobe system of mushroom body (Zars et al., 2000) ellipsoid body \| restricted (Zars et al., 2000) protocerebrum \| lateral & medial (Mehren and Griffith, 2004) median bundle \| restricted (Zars et al., 2000) ring neuron of ellipsoid body \| subset (Zars et al., 2000) ellipsoid body \| subset (Zars et al., 2000) fan-shaped body \| restricted (Zars et al., 2000) large field neuron \| subset of fan-shaped body (Zars et al., 2000) large field neuron \| subset (Zars et al., 2000) optic lobe (Mehren and Griffith, 2004) subesophageal ganglion \| dorso-lateral (Mehren and Griffith, 2004) mushroom body alpha-lobe (Armstrong et al., 1998) mushroom body beta-lobe (Armstrong et al., 1998) adult mushroom body \| restricted (Bushey et al., 2009, Tettamanti et al., 1997) adult mushroom body (Mehren and Griffith, 2004, Sakai and Kitamoto, 2006) adult antennal lobe (Mehren and Griffith, 2004) adult antennal lobe glomerulus \| subset (Zars et al., 2000) gamma Kenyon cell (Pauls et al., 2010) alpha/beta Kenyon cell (Pauls et al., 2010) alpha'/beta' Kenyon cell (Pauls et al., 2010) core layer of the larval mushroom body (Pauls et al., 2010) surface layer of the larval mushroom body (Pauls et al., 2010) inner layer of the larval mushroom body (Pauls et al., 2010) outer layer of the larval mushroom body (Pauls et al., 2010) optic lobe \| restricted (Sakai and Kitamoto, 2006) adult subesophageal ganglion \| restricted (Sakai and Kitamoto, 2006) adult mushroom body gamma-lobe (Armstrong et al., 1998, Aso et al., 2009) adult mushroom body alpha'-lobe (Armstrong et al., 1998) adult pars intercerebralis \| restricted (Crocker et al., 2010) adult brain \| restricted (Crocker et al., 2010) mushroom body beta lobe core stratum \| intense (Aso et al., 2009) mushroom body alpha lobe core stratum \| intense (Aso et al., 2009) mushroom body beta lobe surface stratum \| intense (Aso et al., 2009) mushroom body alpha lobe surface stratum \| intense (Aso et al., 2009) mushroom body beta lobe posterior stratum \| intense (Aso et al., 2009) mushroom body alpha lobe posterior stratum \| intense (Aso et al., 2009) mushroom body alpha' anterior stratum (Aso et al., 2009) mushroom body beta' anterior stratum (Aso et al., 2009) mushroom body alpha' middle stratum (Aso et al., 2009) mushroom body beta' middle stratum (Aso et al., 2009) mushroom body alpha' posterior stratum (Aso et al., 2009) mushroom body beta' posterior stratum (Aso et al., 2009) adult antennal lobe \| faint (Aso et al., 2009) medulla (Aso et al., 2009) lobula (Aso et al., 2009) lobula plate (Aso et al., 2009) adult protocerebrum \| restricted (Aso et al., 2009) lateral horn (Aso et al., 2009) optic tubercle (Aso et al., 2009) inferior neuropils \| faint (Aso et al., 2009) ventrolateral protocerebrum \| faint (Aso et al., 2009) posterior lateral protocerebrum \| faint (Aso et al., 2009) posterior slope \| faint (Aso et al., 2009) pars intercerebralis (Aso et al., 2009) adult antennal nerve \| faint (Aso et al., 2009) adult deuterocerebrum \| faint (Aso et al., 2009) adult tritocerebrum (Aso et al., 2009) adult subesophageal ganglion (Aso et al., 2009) adult stage \| male labellar gland \| restricted (Sakai and Kitamoto, 2006) adult labial sensory neuron \| restricted (Sakai and Kitamoto, 2006) sensory neuron \| subset of prothoracic tarsal segment (Sakai and Kitamoto, 2006)
Additional Information
Statement Reference Scer\GAL4[30Y] drives expression in the mushroom body, scattered expression int he optic lobe and subesophageal ganglion. Expression is also detected in the labellar glands, in both neuronal and non-neuronal cells, and in a subset of sensory neurons of the prothoracic male tarsus. (Sakai and Kitamoto, 2006) Adult expression is observed primarily in the mushroom bodies, subesophageal ganglion, antennal lobes, optic lobes, and lateral protocerebrum, but not in the chemosensory organs. (Mehren and Griffith, 2004) Adult expression is observed in a subset of the antennal glomeruli, in the lobes of mushroom body, in some ring neurons of the ellipsoid body, large field neurons of the fan-shaped body, and lateral fibers of the median bundle. Representative images can be seen Figure 4 of FBrf0127414. (Zars et al., 2000) Expression of Scer\GAL4[30Y] is extensive throughout the adult central brain neuropil. Expression is relatively extensive within the early and late pupal alpha-lobe, but is restricted in the mid-pupal stage. Expression of Scer\GAL4[30Y] is lost at pupal stage 6, but is observed again at pupal stage 7. (Armstrong et al., 1998) Larval expression is observed four Kenyon cells clusters, and in the calyx. In the second and third larval instars, expression is also seen in the pedunculus, in the β and γ' lobes of the mushroom body, and in the circumference of the α and α' lobes. (Tettamanti et al., 1997)

Marker for	antennal glomerulus \| subset (Zars et al., 2000) antennal lobe (Mehren and Griffith, 2004) calyx of mushroom bodystalk of mushroom bodyKenyon cell \| subsetalpha-lobe \| restrictedalpha'-lobe \| restrictedadult mushroom body (Tettamanti et al., 1997) ellipsoid body \| restricted (Zars et al., 2000) fan-shaped body \| restricted (Zars et al., 2000) large field neuron <of> fan-shaped body \| subset (Zars et al., 2000) lateral protocerebrum (Mehren and Griffith, 2004) lobe of mushroom body (Zars et al., 2000) median bundle \| restricted (Zars et al., 2000) mushroom body (Mehren and Griffith, 2004) optic lobe (Mehren and Griffith, 2004) ring neuron <of> ellipsoid body \| subset (Zars et al., 2000) subesophogeal ganglion (Mehren and Griffith, 2004)
Reflects expression of
Reporter construct used in assay	Ecol\lacZ^Scer\UAS.cBa (Armstrong et al., 1998) Fmr1^EP3517 (Bushey et al., 2009) Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} (Crocker et al., 2010, Aso et al., 2009, Pauls et al., 2010)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	30818 w^*; P{GawB}30Y
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	30Y (McGuire et al., 2001, Rosay et al., 2001, Joiner and Griffith, 2000, Connolly et al., 1996, Pitman et al., 2006, Joiner et al., 2006, Mehren and Griffith, 2004, Donlea et al., 2009, Armstrong et al., 1998, Sakai and Kitamoto, 2006) 30y (Zars et al., 2000, Zars et al., 2000, Pauls et al., 2010, Aso et al., 2009) 30Y-GAL4 (Mehren and Griffith, 2004, Tzortzopoulos and Skoulakis, 2007, Ghosh and Feany, 2004, Mehren and Griffith, 2006, Khurana et al., 2006) 30Y-Gal4 (Shafer et al., 2008, Stavropoulos and Young, 2011, Pfeiffenberger and Allada, 2012) 30y-GAL4 (Crocker et al., 2010) P[Gal4]30Y (Belgacem and Martin, 2002, Gatti et al., 2000) P{GAL4:30Y} (Carhan et al., 2005) P{GawB}30Y (Tettamanti et al., 1997, FlyBase, 1992-, Bushey et al., 2009) pGAL4(30Y) (McBride et al., 1999)
Secondary FlyBase IDs

References ( 34 )

Generate a list of	All references relating to this insertion ( 34 )

List References by type	Research paper ( 31 ) Supplementary material ( 1 ) Personal communication to FlyBase ( 1 ) FlyBase analysis ( 1 )
Recent research papers ( 2 )
	Pfeiffenberger and Allada, 2012, PLoS Genet. 8(10): e1003003 Cul3 and the BTB Adaptor Insomniac Are Key Regulators of Sleep Homeostasis and a Dopamine Arousal Pathway in Drosophila. [FBrf0219613] Stavropoulos and Young, 2011, Neuron 72(6): 964--976 insomniac and Cullin-3 Regulate Sleep and Wakefulness in Drosophila. [FBrf0217043] Show all research papers ...