A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}trh10512 Insertion

General Information
Symbol Dmel\P{PZ}trh10512 Species D. melanogaster
Name FlyBase ID FBti0002973
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, trh Viability / fertility
Causes allele(s) Ecol\lacZtrh-10512, trh10512 Stock availability 1 publicly available
LINE ID l(3)10512
Genomic Location
Chromosomal location 3L ( 61C1 ) Sequence location 3L:377,299..377,299 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0050860 FBti0002973 FBti0070417 FBti0050337
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
3L:377,299..377,299 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
61C1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
61C1-61C2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034076
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(Rawlings et al., 2004, Isaac and Andrew, 1996, Haberman et al., 2003, Vosshall, 1998.8.10)
trh
(Schmidt et al., 2011, Mortimer and Moberg, 2007, Wilk et al., 1996)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZtrh-10512
(Isaac and Andrew, 1996, Haberman et al., 2003, Vosshall, 1998.8.10, Rawlings et al., 2004)
trh10512
(Wilk et al., 1996, BDGP Project Members, 1994-1999, Kuo et al., 1996, Isaac and Andrew, 1996, Spradling et al., 1999, Schmidt et al., 2011, Mortimer and Moberg, 2007)
Lethality
References
lethal | embryonic stage | recessive
(Perrimon et al., 1996)
lethal | recessive
(Spradling et al., 1999, Wilk et al., 1996)
partially lethal - majority die | recessive
(Berkeley Drosophila Genome Project, 1993.6.1)
Sterility
References
hide Phenotype Manifest In
abdominal lateral pentascolopidial chordotonal organ lch5 & growth cone
(Harris and Whitington, 2001)
embryonic/first instar larval cuticle
(Perrimon et al., 1996)
embryonic/larval salivary gland | embryonic stage
(Isaac and Andrew, 1996)
embryonic/larval trachea
(Perrimon et al., 1996, Schmidt et al., 2011)
filzkorper
(Isaac and Andrew, 1996)
trachea
(Isaac and Andrew, 1996)
tracheal branch primordium
(Wilk et al., 1996)
tracheal pit
(Isaac and Andrew, 1996)
hide Detailed Description
Statement
Reference
Trachea are absent in homozygous trh[10512] embryos, yet peripheral glial migration phenotype is normal.
(Schmidt et al., 2011)
trh[10512]/+ does not lead to a significant reduction in the penetrance of Scer\GAL4[btl.PS] Vhl[dsRNA.Scer\UAS] tracheal phenotypes.
(Mortimer and Moberg, 2009)
A trh[10512] heterozygous background dominantly suppresses the tracheal phenotypes found in ago[1]/ago[3] mutants.
(Mortimer and Moberg, 2007)
One third of the lateral chordotonal (lch5) neuron clusters fail to project normally beyond the TP1 choice point in trh10512 homozygous mutant embryos. In these segments the growth cones fail to leave the region of the lch5 cluster or they extend ventrally but along an aberrant route. Occasionally, the lch5 axons fasciculate with the axon of v\'ch1 and contribute to the segmental nerve (SN). In most hemisegments of trh10512 mutant embryos, the lch5 axon bundle does reach the intersegmental nerve (ISN) and the morphology of the fascicle appears normal in most cases.
(Harris and Whitington, 2001)
Salivary glands form closed sacs that are not connected to the foregut. The filzkorper do not elongate and tracheal pits do not form. The oesophagus, gastric caecae and Malpighian tubules are unaffected.
(Isaac and Andrew, 1996)
Germline clones produce eggs with patterning defects: trachea are missing.
(Perrimon et al., 1996)
Tracheal structures are not formed in the embryo. Phenotype is more severe than for trh1.
(Wilk et al., 1996)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage  
embryonic/larval trachea  
(Isaac and Andrew, 1996)
filzkorper  
(Isaac and Andrew, 1996)
embryonic/larval salivary gland  
(Isaac and Andrew, 1996)
central nervous system | restricted  
(Isaac and Andrew, 1996)
Additional Information
Statement
Reference
High level enhancer trap expression is observed in the whole salivary gland in both duct and secretory cells. Expression is also seen in all tracheal cells, in the filzkorper, and in a subset of CNS cells.
(Isaac and Andrew, 1996)
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)10512
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11747
P{PZ}trh10512 ry506/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3L:360831-360832 confirmed by FlyBase alignment of dbGSS accession AQ034076 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{PZ}trh10512
(FlyBase, 2005, FlyBase, 1992-, )
P{ry+t7.2=PZ}trh10512
(FlyBase, 1992-)
trh10512
(Schmidt et al., 2011, Mortimer and Moberg, 2007)
trh lacZ
(Haberman et al., 2003)
trh-lacZ
(Rawlings et al., 2004)
Secondary FlyBase IDs
  • FBti0000745
hide References ( 22 )
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hide Recent research papers ( 1 )
Schmidt et al., 2011, Glia 59(9): 1264--1272
Transcriptional regulation of peripheral glial cell differentiation in the embryonic nervous system of drosophila. [FBrf0214270]
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