Dmel\P{PZ}Ras85D⁰⁵⁷⁰³ Insertion

General Information
Symbol	Dmel\P{PZ}Ras85D⁰⁵⁷⁰³	Species	D. melanogaster
Name		FlyBase ID	FBti0002988
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data
Affected gene(s)	Ecol\lacZ, Ras85D	Viability / fertility
Causes allele(s)	Ecol\lacZ^Ras85D-05703, Ras85D⁰⁵⁷⁰³	Stock availability	none publicly available
LINE ID	fs(3)05703
Genomic Location
Chromosomal location	3R ( 85D19 )	Sequence location	3R:5,338,761..5,338,761
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:5,338,761..5,338,761 (Schnorr and Berg, 1996)
Orientation
Cytological location (computed by FlyBase)	85D19 ( inferred by FlyBase from sequence location )
Cytological location (reported)	85D1-85D8 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Ras85D (Schnorr and Berg, 1996, Schnorr and Berg, 1996)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Ras85D-05703 (BDGP Project Members, 1994-1999) Ras85D⁰⁵⁷⁰³ (Rorth, 1996, BDGP Project Members, 1994-1999, Schnorr and Berg, 1996)

Lethality References partially lethal - majority die (Schnorr and Berg, 1996) partially lethal - majority die \| recessive (Rorth, 1996)
Sterility References female sterile (Schnorr and Berg, 1996) female sterile \| recessive (Rorth, 1996)
Phenotype Manifest In
	abdominal segment 1 (Schnorr and Berg, 1996) abdominal segment 2 (Schnorr and Berg, 1996) abdominal segment 3 (Schnorr and Berg, 1996) abdominal segment 4 (Schnorr and Berg, 1996) abdominal segment 5 (Schnorr and Berg, 1996) abdominal segment 6 (Schnorr and Berg, 1996) abdominal segment 7 (Schnorr and Berg, 1996) abdominal segment 8 (Schnorr and Berg, 1996) abdominal segment 9 (Schnorr and Berg, 1996) abdominal segment 10 (Schnorr and Berg, 1996) abdominal segment 11 (Schnorr and Berg, 1996) dorsal appendage (Schnorr and Berg, 1996) dorsal appendage \| maternal effect (Ward and Berg, 2005) egg chamber (Schnorr and Berg, 1996) embryonic/first instar larval cuticle (Schnorr and Berg, 1996) female terminalia (Schnorr and Berg, 1996) head (Schnorr and Berg, 1996) male terminalia (Schnorr and Berg, 1996) nurse cell (Schnorr and Berg, 1996) ommatidium (Schnorr and Berg, 1996) oocyte (Schnorr and Berg, 1996) ovary (Schnorr and Berg, 1996) type I bouton (Koh et al., 2002)
Detailed Description
Statement Reference Ras85D⁰⁵⁷⁰³/+; sli²/+ embryos produce a mild heart development phenotype. (MacMullin and Jacobs, 2006) 100% of the eggs laid by Ras85D⁰⁵⁷⁰³/Ras85D^e1B mothers have a single central dorsal appendage. (Ward and Berg, 2005) The Ras85D⁰⁵⁷⁰³ mutant result in a significant reduction in the number of type I synaptic boutons compared with wild-type. (Koh et al., 2002) Dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies. (Zhang et al., 1999) Expression of Gap1^EP45 in homozygous Ras85D⁰⁵⁷⁰³ flies using Scer\GAL4^hs.2sev produces severely disorganised eyes. (Rorth, 1996) Homozygotes have no detectable eye defects. (Rorth, 1996) Expression of Rlb1^hs.PE fails to ameliorate the phenotype. (Schnorr and Berg, 1996) A low percentage of egg chambers from homozygous females degenerate between stages 8 and 10. Some egg chambers contain too few nurse cells or fail to correctly position the developing oocyte within the egg chamber. Female transheterozygotes with Df(3R)by10 have rudimentary ovaries. All late stage egg chambers lack dorsal appendage structures and possess only a patch of chorion centered on the dorsal midline. Few eggs are laid, eggs appear ventralised but their overall shape is normal. Homozygous eyes exhibit slightly disrupted ommatidial array. Ras85D^ΔC17b/Ras85D⁰⁵⁷⁰³ and Df(3R)ΔC40b/Ras85D^e1B transheterozygotes are lethal. Ras85D^e1B/Ras85D⁰⁵⁷⁰³ and Ras85D^e2F/Ras85D⁰⁵⁷⁰³ transheterozygotes are semi-lethal. Ras85D^Sutor-341/Ras85D⁰⁵⁷⁰³ and Ras85D^Sutor-404/Ras85D⁰⁵⁷⁰³ transheterozygotes have low viability. Female transheterozygotes with Ras85D^e2F, Ras85D^Sutor-341 or Ras85D^Sutor-404 lay eggs with a severely disrupted eggshell (patch of chorion instead of dorsal appendages), 57%, 40% and 25% of eggs (respectively) hatch and develop to normal larvae. Unhatched Ras85D⁰⁵⁷⁰³/Ras85D^e2F embryos have normal cuticles, or are missing elements of the head skeleton or have defects in the anterior and posterior termini, holes in the cuticle and disordered posterior segments. Female transheterozygotes with Ras85D^e1B lay eggs with a single dorsal appendage, 84% of eggs hatch and develop to normal larvae. Transheterozygotes with Ras85D^e2F, Ras85D^Sutor-341 or Ras85D^Sutor-404 exhibit slightly disrupted ommatidial array (small number of fused and misplaced ommatidia). Transheterozygotes with Ras85D^e1B exhibit extremely rough eyes (many fused and misplaced ommatidia). (Schnorr and Berg, 1996) Mutant phenotypes are completely rescued by Ras85D^+t5.5, restoring viability and proper ovarian development. Ras85D^Δ fails to rescue the mutant phenotypes. (Schnorr and Berg, 1996) female-sterile.
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	fs(3)05703 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	P{PZ} insertion is located 28bp upstream of a putative Ras85D transcription start site. (Schnorr and Berg, 1996) This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}Ras85D⁰⁵⁷⁰³ (FlyBase, 1992-, Schnorr and Berg, 1996, )
Secondary FlyBase IDs

References ( 13 )
Research paper	MacMullin and Jacobs, 2006, Dev. Biol. 293(1): 154--164 Slit coordinates cardiac morphogenesis in Drosophila. [FBrf0190142] Ward and Berg, 2005, Mech. Dev. 122(2): 241--255 Juxtaposition between two cell types is necessary for dorsal appendage tube formation. [FBrf0184135] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Koh et al., 2002, J. Neurosci. 22(7): 2496--2504 The Ras1-mitogen-activated protein kinase signal transduction pathway regulates synaptic plasticity through fasciclin II-mediated cell adhesion. [FBrf0151505] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Zhang et al., 1999, Genetics 151(2): 697--711 A genetic screen for modifiers of Drosophila Src42A identifies mutations in egfr, rolled and a novel signaling gene. [FBrf0107534] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768] Schnorr and Berg, 1996, Genetics 144(4): 1545--1557 Differential activity of Ras1 during patterning of the Drosophila dorsoventral axis. [FBrf0091163] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}Ras85D05703 Insertion

Dmel\P{PZ}Ras85D⁰⁵⁷⁰³ Insertion