Dmel\P{lacW}oho48A^k06524 Insertion

General Information
Symbol	Dmel\P{lacW}oho48A^k06524	Species	D. melanogaster
Name		FlyBase ID	FBti0003180
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, oho48A	Viability / fertility
Causes allele(s)	Ecol\lacZ^{oho48A-k06524}, oho48A^k06524	Stock availability	2 publicly available
LINE ID	l(2)k06524
Genomic Location
Chromosomal location	2R ( 48B2 )	Sequence location	2R:7,527,927..7,527,927 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:7,527,927..7,527,927 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	48B2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	48B1--48B2 (in situ hybridization reported) (Roote, 2004.6.24) 48B1-48B2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025799 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) oho48A (Torok et al., 1993)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^{oho48A-k06524} (BDGP Project Members, 1994-1999) oho48A^k06524 (Torok et al., 1993, BDGP Project Members, 1994-1999, Spradling et al., 1999)

Lethality References lethal \| larval stage (Torok et al., 1993) lethal \| recessive (Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	embryonic/larval brain (Torok et al., 1993) embryonic/larval lymph gland (Torok et al., 1993)
Detailed Description
Statement Reference Brain and discs are smaller than wild type. Lymph glands retain lobular structure. (Torok et al., 1993)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k06524 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10631 y¹ w^67c23; P{lacW}oho48A^k06524/CyO
Kyoto	111200 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}oho48A^k06524 /CyO y⁺
Linkouts
Comments
	Location 2R:7156329-7156330 confirmed by FlyBase alignment of dbGSS accession AQ025799 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}k06524 (Roote, 2004.6.24) P{lacW}oho48A^k06524 (FlyBase, 2005, Torok et al., 1993, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 11 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Daborn et al., 2001, Mol. Genet. Genomics 266(4): 556--563 DDT resistance in Drosophila correlates with Cyp6g1 over-expression and confers cross-resistance to the neonicotinoid imidacloprid. [FBrf0145128] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Torok et al., 1993, Genetics 135(1): 71--80 P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. [FBrf0058618] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Roote, 2004.6.24, Cam Dps. Cam Dps. [FBrf0178882] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}oho48Ak06524 Insertion

Dmel\P{lacW}oho48A^k06524 Insertion