A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}GlyPk07918 Insertion

General Information
Symbol Dmel\P{lacW}GlyPk07918 Species D. melanogaster
Name FlyBase ID FBti0003181
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, GlyP Viability / fertility viable
Causes allele(s) Ecol\lacZGlyP-k07918, GlyPk07918 Stock availability 2 publicly available
LINE ID l(2)k07918
Genomic Location
Chromosomal location 2L ( 22C3 ) Sequence location 2L:2,130,807..2,130,807 [+]
Map ( GBrowse )
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:2,130,807..2,130,807 [+]
(Tick et al., 1999)
2L:2,130,799..2,130,799 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
22C3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
22B6-22B7 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025849
(FlyBase, 1992-,  
AQ073302
(FlyBase, 1992-)
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Gelbart, 1999.7.20)
GlyP
(Tick et al., 1999, Torok et al., 1993, Tick et al., 1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZGlyP-k07918
(BDGP Project Members, 1994-1999, Gelbart, 1999.7.20)
GlyPk07918
(Tick et al., 1999, Spradling et al., 1999)
Lethality
References
viable
(Gelbart, 1999.7.20, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
No obvious phenotype.
(Spradling et al., 1999)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k07918
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10692
y1 w67c23; P{lacW}GlyPk07918 ftk07918/CyO
Kyoto
111230
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}GlyPk07918 ftk07918 P{neoFRT}40A/CyO y+
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hide Comments
Fertility not tested.Insertion is separable from lethal originally isolated on same chromosome.
(Gelbart, 1999.7.20)
Insertion separable from lethal originally isolated on the same chromosome.
(Spradling et al., 1999)
The insertion site is reported at -52 with respect to the start of GlyP transcription. The P insertion is in a negative orientation with respect to GlyP gene transcription.
(Tick et al., 1999)
Separable from ftk07918.
(BDGP Project Members, 1994-1999)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}GlyPk07918
(FlyBase, 1992-, Tick et al., 1999)
P{lacW}k07918
(FlyBase, 1992-)
P{lacW}l(2)k07918k07918
(Torok et al., 1993, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Tick et al., 1999, Biochem. biophys. Res. Commun. 257(1): 34--43
Structural and functional characterization of the Drosophila glycogen phosphorylase gene. [FBrf0108288]
Torok et al., 1993, Genetics 135(1): 71--80
P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. [FBrf0058618]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gelbart, 1999.7.20, BDGP lines.
BDGP lines. [FBrf0109761]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]