Dmel\P{lacW}prod^k08810 Insertion

General Information
Symbol	Dmel\P{lacW}prod^k08810	Species	D. melanogaster
Name		FlyBase ID	FBti0003184
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, prod	Viability / fertility
Causes allele(s)	Ecol\lacZ^prod-k08810, prod^k08810	Stock availability	2 publicly available
LINE ID	l(2)k08810
Genomic Location
Chromosomal location	2R ( 56A2 )	Sequence location	2R:14,858,525..14,858,525 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:14,858,525..14,858,525 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	56A2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	56A1-56A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025870 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) prod (Torok et al., 1993)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^prod-k08810 (BDGP Project Members, 1994-1999) prod^k08810 (Torok et al., 1993, BDGP Project Members, 1994-1999, Spradling et al., 1999, Torok et al., 1997)

Lethality References lethal \| larval stage (Torok et al., 1993) lethal \| larval stage \| recessive (Torok et al., 1997) lethal \| recessive (Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	adult cuticle \| somatic clone (Torok et al., 1997) embryonic/larval brain (Torok et al., 1997, Torok et al., 1993) embryonic/larval lymph gland (Torok et al., 1997, Torok et al., 1993) eye-antennal disc (Torok et al., 1993) imaginal disc (Torok et al., 1997) leg & adult cuticle \| somatic clone (Torok et al., 1997) leg & macrochaeta \| somatic clone (Torok et al., 1997) macrochaeta \| somatic clone (Torok et al., 1997) mitosis & nuclear chromosome (Torok et al., 1997)
Detailed Description
Statement Reference Homozygotes die as third instar larvae after a prolonged 20-day larval period. Homozygous larvae have a slower growth rate than normal, and do not reach full size until approximately 4 days after their heterozygous siblings pupariate. 9 day old larvae have tiny, slow-growing imaginal discs, which lose their epithelial organisation as the larvae age. There is massive cell death in the imaginal discs, and dead cells and cell fragments accumulate at the basal surface of the disc epithelium. Blood cells also accumulate around the discs. The first pair of lymph gland lobes begins to melanise at around 9 days after egg laying, and this melanisation spreads posteriorly as the larvae age until all the lobes are melanised and start to disintegrate. 20 day old larvae show a striking accumulation of amorphous tissue in the head region around and inside the imaginal discs, which consists of dead cell fragments and blood cells. The brain is slow-growing and smaller than normal. It develops an abnormal shape and contributes to the amorphous mass of tissue in the 20 day old larva. The penetrance of the mutant phenotype is 100%, but the expressivity is variable, with the degree of melanisation and cell death varying between different larvae. Homozygous clones (that are Minute⁺ in a Minute heterozygous background) in the leg have wrinkled cuticle and defective bristles. Homozygous larval tissue has a significantly lower mitotic index than wild-type, which decreases as the larvae age, until there are hardly any mitotic cells in 2 week old larvae. The metaphase/anaphase ratio in is 16:1 in mutant larval neuroblasts 5 days after egg laying, compared to 4.6:1 in wild-type. Incomplete chromosome condensation is seen, especially near the centromeres. (Torok et al., 1997) The mutant phenotype and lethality are completely rescued by prod^+t8. prod^Scer\UAS.cTa partially rescues the mutant phenotype when expressed using Scer\GAL4^69B; the lethal phase shifts to the pupal or pharate adult stages, and the larvae have relatively normal imaginal discs with only slightly abnormal folding. (Torok et al., 1997) Brain is smaller than wild type with elongated hemispheres. Discs form structureless masses, sometimes fusing with each other and the brain. The eye-antenna discs are larger than others, neoplastic outgrowths. Hyperplastic lymph glands grow as diffuse masses that fills the anterior body cavity, infrequent melanotic masses in larvae. (Torok et al., 1993)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k08810 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10814 y¹ w^67c23; P{lacW}prod^k08810/CyO
Kyoto	111248 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}prod^k08810 /CyO y⁺
Linkouts
Comments
	Location 2R:14485883-14485884 confirmed by FlyBase alignment of dbGSS accession AQ025870 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}prod^k08810 (FlyBase, 2005, Torok et al., 1993, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Torok et al., 1997, Genes Dev. 11(2): 213--225 The product of proliferation disrupter is concentrated at centromeres and required for mitotic chromosome condensation and cell proliferation in Drosophila. [FBrf0091413] Torok et al., 1993, Genetics 135(1): 71--80 P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. [FBrf0058618] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}prodk08810 Insertion

Dmel\P{lacW}prod^k08810 Insertion