Dmel\P{lwB}sr^B14.0 Insertion

General Information
Symbol	Dmel\P{lwB}sr^B14.0	Species	D. melanogaster
Name		FlyBase ID	FBti0003279
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lwB}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, sr	Viability / fertility
Causes allele(s)	Ecol\lacZ^sr-B14.0, sr^B14.0	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 90E4-90F1 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	90E4-90F1 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lwB}
Location-dependent role	lacZ enhancer trap (Wilson et al., 1989)
Size	12.486Kb
Associated alleles	Ecol\lacZ^P\T.A92a w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Martin-Bermudo and Brown, 1996, Volk and Vijay Raghavan, 1994, Martin-Bermudo and Brown, 2000, Fernandes and Vijay Raghavan, 1993, Martin-Bermudo, 2000, Halfon and Keshishian, 1998, Lee et al., 1995, Fernandes et al., 1996, Sandstrom et al., 1997) sr (Volk and Vijay Raghavan, 1994)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^sr-B14.0 (Martin-Bermudo and Brown, 1996, Volk and Vijay Raghavan, 1994, Martin-Bermudo and Brown, 2000, Fernandes and Vijay Raghavan, 1993, Martin-Bermudo, 2000, Halfon and Keshishian, 1998, Lee et al., 1995, Fernandes et al., 1996, Sandstrom et al., 1997) sr^B14.0 (Volk and Vijay Raghavan, 1994, Lee et al., 1995, Beiman et al., 1996, Martin-Bermudo and Brown, 1996)

Lethality References viable (Beiman et al., 1996)
Sterility References
Phenotype Manifest In
	adult muscle system (Beiman et al., 1996) dorsal medial muscle (Lee et al., 1995) macrochaeta (Volk and Vijay Raghavan, 1994)
Detailed Description
Statement Reference Defects in adult muscle patterning. (Beiman et al., 1996) Homozygotes exhibit a significant reduction in DLM size. (Lee et al., 1995) Weak whorl on dorsal thoracic bristles. (Volk and Vijay Raghavan, 1994)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference third instar larval stage wing disc (Lee et al., 1995) muscle attachment site \| presumptive (Lee et al., 1995) pupal stage muscle attachment site (Lee et al., 1995)
Additional Information
Statement Reference Enhancer trap expression is observed in pupae in the dorsal longitudinal muscle insertion sites in the epidermis but not in the developing muscle fibers. In third instar larval wing discs, staining is observed in cells fated to be muscle attachment sites but not in myoblasts. (Lee et al., 1995)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lwB}sr^B14.0 (Volk and Vijay Raghavan, 1994, FlyBase, 1992-) stripe-lacZ (Martin-Bermudo and Brown, 2000)
Secondary FlyBase IDs

References ( 12 )
Research paper	Martin-Bermudo and Brown, 2000, J. Cell Sci. 113(21): 3715--3723 The localized assembly of extracellular matrix integrin ligands requires cell-cell contact. [FBrf0130272] Martin-Bermudo, 2000, Development 127(12): 2607--2615 Integrins modulate the Egfr signaling pathway to regulate tendon cell differentiation in the Drosophila embryo. [FBrf0128565] Halfon and Keshishian, 1998, Dev. Biol. 199(1): 164--174 The Toll pathway is required in the epidermis for muscle development in the Drosophila embryo. [FBrf0103273] Sandstrom et al., 1997, Dev. Biol. 181(2): 168--185 Broad-complex transcription factors regulate thoracic muscle attachment in Drosophila. [FBrf0091319] Beiman et al., 1996, Genes Dev. 10(23): 2993--3002 Heartless, a Drosophila FGF receptor homolog, is essential for cell migration and establishment of several mesodermal lineages. [FBrf0090449] Fernandes et al., 1996, Dev. Biol. 176(2): 166--184 Development of the indirect flight muscle attachment sites in Drosophila: role of the PS integrins and the stripe gene. [FBrf0088109] Martin-Bermudo and Brown, 1996, J. Cell Biol. 134(1): 217--226 Intracellular signals direct integrin localization to sites of function in embryonic muscles. [FBrf0089738] Lee et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92(22): 10344--10348 Identification of a Drosophila muscle development gene with structural homology to mammalian early growth response transcription factors. [FBrf0084118] Volk and Vijay Raghavan, 1994, Development 120(1): 59--70 A central role for epidermal segment border cells in the induction of muscle patterning in the Drosophila embryo. [FBrf0068724] Wilson et al., 1989, Genes Dev. 3(9): 1301--1313 P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila. [FBrf0049802]
Abstract	Fernandes and Vijay Raghavan, 1993, Davis, Zipursky, 1993: 30 Development of the indirect flight muscles of Drosophila melanogaster. [FBrf0078374]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lwB}srB14.0 Insertion

Dmel\P{lwB}sr^B14.0 Insertion