Dmel\P{lacW}Syt1^T77 Insertion

General Information
Symbol	Dmel\P{lacW}Syt1^T77	Species	D. melanogaster
Name		FlyBase ID	FBti0003285
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Syt1	Viability / fertility
Causes allele(s)	Ecol\lacZ^Syt1-T77, Syt1^T77	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2L ( 23A6-23B1 )	Sequence location
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation	- (Littleton et al., 1993)
Cytological location (computed by FlyBase)	23A6-23B1 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Littleton and Bellen, 1994) Syt1 (Littleton et al., 1993)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Syt1-T77 (Littleton and Bellen, 1994) Syt1^T77 (Littleton et al., 1993, Littleton and Bellen, 1994, Ruden et al., 2000, Littleton et al., 1994, Littleton et al., 1995)

Lethality References lethal \| recessive (Littleton et al., 1993)
Sterility References
Phenotype Manifest In
	egg \| germline clone (Ruden et al., 2000)
Detailed Description
Statement Reference Germ-line clones of this mutant often produce miniature eggs with greatly reduced surface volumes. (Ruden et al., 2000) No obvious morphological defects. (Littleton and Bellen, 1994) Transheterozygote combinations with other syt alleles are lethal. (Littleton et al., 1994)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Transposed descendant of	P{lacW}B8-2-30
Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	4377 y¹ w^*; P{lacW}Syt1^T77/CyO
Linkouts
Comments
	Local hop (replicative): P{lacW}B8-2-30 insertion still present on the original P{lacW}syt^T77 chromosome. (Littleton et al., 1993)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}Syt1^T77 P{lacW}syt^T77 (FlyBase, 1992-, Littleton et al., 1993)
Secondary FlyBase IDs

References ( 7 )
Research paper	Ruden et al., 2000, Dev. Biol. 218(2): 314--325 Membrane fusion proteins are required for oskar mRNA localization in the Drosophila egg chamber. [FBrf0125279] Littleton et al., 1995, J. Neurochem. 65(1): 32--40 Immunocytochemical analysis of axonal outgrowth in synaptotagmin mutations. [FBrf0083593] Littleton and Bellen, 1994, Genetics 138(1): 111--123 Genetic and phenotypic analysis of thirteen essential genes in cytological interval 22F1-2; 23B1-2 reveals novel genes required for neural development in Drosophila. [FBrf0076536] Littleton et al., 1994, Proc. Natl. Acad. Sci. USA 91(23): 10888--10892 Calcium dependence of neurotransmitter release and rate of spontaneous vesicle fusions are altered in Drosophila synaptotagmin mutants. [FBrf0077252] Littleton et al., 1993, Cell 74(6): 1125--1134 Mutational analysis of Drosophila synaptotagmin demonstrates its essential role in Ca²⁺-activated neurotransmitter release. [FBrf0064585] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{lacW}Syt1T77 Insertion

Dmel\P{lacW}Syt1^T77 Insertion