Dmel\P{lwB}aos^W11 Insertion

General Information
Symbol	Dmel\P{lwB}aos^W11	Species	D. melanogaster
Name		FlyBase ID	FBti0003321
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lwB}	Expression data	lacZ reporter
Affected gene(s)	aos, Ecol\lacZ	Viability / fertility
Causes allele(s)	aos^W11, Ecol\lacZ^aos-W11	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3L ( 73A2 )	Sequence location
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	73A2 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lwB}
Location-dependent role	lacZ enhancer trap (Wilson et al., 1989)
Size	12.486Kb
Associated alleles	Ecol\lacZ^P\T.A92a w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	aos (Freeman et al., 1992, Salzer et al., 2010) Ecol\lacZ (Freeman et al., 1992, Klaes et al., 1994, Wildonger et al., 2005, Roch et al., 2002, Adachi-Yamada, 2002, Stemerdink and Jacobs, 1997, Dong and Jacobs, 1997, Dumstrei et al., 1998, Wemmer and Klaembt, 1995, Scholz et al., 1997, Pallavi and Shashidhara, 2003, Scholz et al., 1997, Lam et al., 2006, Miura et al., 2008, Yan et al., 2009, Rendina et al., 2010, Ajuria et al., 2011)
Alleles and Phenotypes
Causes alleles	aos^W11 (Freeman et al., 1992, Brunner et al., 1994, Stemerdink and Jacobs, 1997, Brown and Freeman, 2003, Weber et al., 2003, Wemmer and Klaembt, 1995, Dworkin and Gibson, 2006, Salzer et al., 2010) Ecol\lacZ^aos-W11 (Freeman et al., 1992, Klaes et al., 1994, Wildonger et al., 2005, Roch et al., 2002, Adachi-Yamada, 2002, Stemerdink and Jacobs, 1997, Dong and Jacobs, 1997, Dumstrei et al., 1998, Wemmer and Klaembt, 1995, Scholz et al., 1997, Pallavi and Shashidhara, 2003, Scholz et al., 1997, Lam et al., 2006, Miura et al., 2008, Yan et al., 2009, Rendina et al., 2010, Ajuria et al., 2011)

Lethality References lethal \| recessive (Dworkin and Gibson, 2006) viable (Wemmer and Klaembt, 1995)
Sterility References
Phenotype Manifest In
	cone cell (Freeman et al., 1992) dorsal appendage (Wasserman and Freeman, 1998) dorsal appendage \| maternal effect (Zartman et al., 2009) eye (Zartman et al., 2009) eye photoreceptor cell (Freeman et al., 1992) interommatidial bristle & eye \| precursor (Freeman et al., 1992) midline glial cell (Stemerdink and Jacobs, 1997) mystery cell (Freeman et al., 1992) ommatidium (Gaengel and Mlodzik, 2003, Freeman, 1994) ommatidium \| somatic clone (Brown and Freeman, 2003) optic lobe (Freeman et al., 1992) photoreceptor cell \| ectopic (Gaengel and Mlodzik, 2003) pigment cell (Freeman, 1994) retina (Brunner et al., 1994) wing (Dworkin and Gibson, 2006)
Detailed Description
Statement Reference argos[Δ7]/argos[W11] flies have blisters in the eye. Only 5% of eggs derived from argos[Δ7]/argos[W11] have defects in dorsal appendage morphology, which include a reduced distance between the two appendages and appendages that are shorter than normal. (Zartman et al., 2009) Heterozygotes show a direct effect on the shape of the wing. (Dworkin and Gibson, 2006) The ectopic wing vein phenotype caused by expression of Socs44A[Scer\UAS.cRa] under the control of Scer\GAL4[en-e16E] is enhanced by argos[W11]/+. (Rawlings et al., 2004) When homozygous mutant clones are made in the eye, although many of the mutant ommatidia have too many photoreceptors, a significant proportion have the correct number. many of these ommatidia are misrotated. (Brown and Freeman, 2003) Mutant eye discs show, in addition to the extra R-cell phenotype, severe ommatidial rotation defects, though the initial 45^oC rotation seen in wild-type is less affected. (Gaengel and Mlodzik, 2003) argos^W11 enhances the ed^k01102 phenotype; the number of ommatidia with multiple R8 cells is increased from 21% to 46% in the double mutant flies. (Spencer and Cagan, 2003) The Cct1¹⁶⁹¹⁹ defective eye phenotype is not significantly enhanced in Cct1¹⁶⁹¹⁹/+, argos^W11/+, N^55e11/+ triple heterozygotes. (Weber et al., 2003) argos^W11 enhances the extra wing vein phenotype of Egfr^E1/Egfr^E3 transheterozygotes, but suppresses the eye phenotype. (Lesokhin et al., 1999) argos^Δ7/argos^W11 hypomorphic females lay a significant proportion of eggs with fused dorsal appendages. Larvae hatch from the eggs, with dorsal-ventral pattern unperturbed. (Wasserman and Freeman, 1998) The number of midline glia cells is increased to an average of 5.1 +/- 0.2 cells per segment in homozygous embryos. (Stemerdink and Jacobs, 1997) No effect on the faf eye phenotype. (Huang and Fischer-Vize, 1996) Retinal degeneration in aging flies. (Brunner et al., 1994) Mutant ommatidia have extra pigment cells. (Freeman, 1994) Reduced viability and rough eye phenotype, including blistering in the posterior region of the eye due to a lawn of undifferentiated cells in the pupal eye. Eyes have abnormal rhabdomere morphology and extra outer photoreceptors. Optic lobes are small and disorganised. Many of the mystery cells start differentiating as neurons and never leave the developing ommatidia. Extra cone cells and an abnormally high number of primary pigment cells are found in the pupal eye. Interommatidial bristle precursors are disorganised though the total number appears to be normal. (Freeman et al., 1992)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage \| late midline glial cell (Klaes et al., 1994) embryonic stage organism \| pair rule expression pattern (Freeman et al., 1992) midline glial cell (Freeman et al., 1992) embryonic stage 9 -- 16 epidermis \| ventral (Freeman et al., 1992) third instar larval stage cone cell (Freeman et al., 1992) eye-antennal disc (Freeman et al., 1992) primary pigment cell (Freeman et al., 1992) photoreceptor cell R1 -- photoreceptor cell R8 (Freeman et al., 1992) adult stage lamina \| restricted (Freeman et al., 1992) cone cell (Freeman et al., 1992)
Additional Information
Statement Reference Enhancer trap expression is observed in the developing photoreceptors and cone cells in the third instar eye imaginal disc. It is first expressed behind the morphogenetic furrow and is switched on at around the same time as the first neural anitgens. Thus expression occurs in the cells in the order in which they differentiate, R8, then R2 and R5, R3 and R4, R1 and R6, R7, and finally cone cells. In pupae, expression is observed in cone cells and in primary pigment cells. In adults expression is observed in cells which are probably cone cells as well as in a subset of cells in the optic lobe and brain. In embryos, expression is first observed in the cellular blastoderm in dorsal regions at the anterior and posterior ends and in the dorsal ectoderm anlage, in a pair-rule pattern. At stage 9, expression occurs in two rows of ectodermal cells bordering the mesectoderm. As the mesectoderm invaginates, the rows meet and expression occurs in the entire ventral ectoderm. In germ band extended embryos, additional expression is seen in the head region and in segmentally repeated groups of ectodermal cells. Expression is observed in dorsal and lateral epidermal cells located at the segment boundary and in all ventral epidermal cells until stage 16. As the germ band retracts, expression is seen in the midline glial cells. (Freeman et al., 1992)

Marker for
Reflects expression of	aos (Freeman et al., 1992)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	2513 w⁸; P{lwB}aos^W11/TM3, Sb¹
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	aos^w11-lacZ (Wildonger et al., 2005) aos-lacZ (Pallavi and Shashidhara, 2003, Adachi-Yamada, 2002, Roch et al., 2002, Yan et al., 2009) aos-lacZ^W11 (Miura et al., 2008, Rendina et al., 2010) argos^w11 (Ajuria et al., 2011) argos^w11lacZ (Roch et al., 2002) argos-lacZ (Yan et al., 2009) P{A92}W11 (FlyBase, 1992-) P{lacZ}W11 (FlyBase, 1992-) P{lwB}aos^W11 P{lwB}argos^W11 (FlyBase, 1992-) P{lwB}gil^W11 (Freeman et al., 1992, FlyBase, 1992-) P{w^+mW.hs=lwB}gil^W11 (FlyBase, 1992-) W11 (Klaes et al., 1994)
Secondary FlyBase IDs
	FBti0001275 FBti0002149
References ( 32 )

Generate a list of	All references relating to this insertion ( 32 )

List References by type	Research paper ( 30 ) Erratum ( 1 ) FlyBase analysis ( 1 )
Recent research papers ( 3 )
	Ajuria et al., 2011, Development 138(5): 915--924 Capicua DNA-binding sites are general response elements for RTK signaling in Drosophila. [FBrf0212975] Rendina et al., 2010, Genetics 186(1): 167--181 Bap170, a Subunit of the Drosophila PBAP Chromatin Remodeling Complex, Negatively Regulates the EGFR Signaling. [FBrf0211845] Salzer et al., 2010, Genetics 184(1): 185--197 The retinal determination gene eyes absent is regulated by the EGF receptor pathway throughout development in Drosophila. [FBrf0209690] Show all research papers ...

Dmel\P{lwB}aosW11 Insertion

Dmel\P{lwB}aos^W11 Insertion