Dmel\P{lacW}lola^5D2 Insertion

General Information
Symbol	Dmel\P{lacW}lola^5D2	Species	D. melanogaster
Name		FlyBase ID	FBti0003339
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	lola	Viability / fertility
Causes allele(s)	lola^5D2	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2R ( 47A11-47A13 )	Sequence location
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	47A11-47A13 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	lola (Giniger et al., 1994, Paige Bass et al., 2007)
Alleles and Phenotypes
Causes alleles	lola^5D2 (Giniger et al., 1994, Madden et al., 1999, Paige Bass et al., 2007, Horiuchi et al., 2003, Cavarec et al., 1997)

Lethality References lethal (Paige Bass et al., 2007) lethal \| recessive (Giniger et al., 1994)
Sterility References
Phenotype Manifest In
	abdominal 1 lateral monoscolopidial chordotonal organ lch1 (Giniger et al., 1994) abdominal 1 lateral pentascolopidial chordotonal organ lch5 (Giniger et al., 1994) abdominal 2 lateral monoscolopidial chordotonal organ lch1 (Giniger et al., 1994) abdominal 2 lateral pentascolopidial chordotonal organ lch5 (Giniger et al., 1994) abdominal 3 lateral monoscolopidial chordotonal organ lch1 (Giniger et al., 1994) abdominal 3 lateral pentascolopidial chordotonal organ lch5 (Giniger et al., 1994) abdominal 4 lateral monoscolopidial chordotonal organ lch1 (Giniger et al., 1994) abdominal 4 lateral pentascolopidial chordotonal organ lch5 (Giniger et al., 1994) abdominal 5 lateral monoscolopidial chordotonal organ lch1 (Giniger et al., 1994) abdominal 5 lateral pentascolopidial chordotonal organ lch5 (Giniger et al., 1994) abdominal 6 lateral monoscolopidial chordotonal organ lch1 (Giniger et al., 1994) abdominal 6 lateral pentascolopidial chordotonal organ lch5 (Giniger et al., 1994) abdominal 7 lateral monoscolopidial chordotonal organ lch1 (Giniger et al., 1994) abdominal 7 lateral pentascolopidial chordotonal organ lch5 (Giniger et al., 1994) abdominal intersegmental nerve (Giniger et al., 1994) abdominal segmental nerve (Madden et al., 1999) adult antennal lobe projection neuron \| somatic clone (Spletter et al., 2007) axon & adult antennal lobe projection neuron \| somatic clone (Spletter et al., 2007) commissure (Giniger et al., 1994) dendrite & adult antennal lobe projection neuron \| somatic clone (Spletter et al., 2007) egg chamber \| germline clone (Paige Bass et al., 2007) intersegmental nerve (Madden et al., 1999) longitudinal connective (Giniger et al., 1994) nurse cell \| germline clone (Paige Bass et al., 2007) VUM1 neuron (Giniger et al., 1994) VUM2 neuron (Giniger et al., 1994) VUM3 neuron (Giniger et al., 1994) VUM4 neuron (Giniger et al., 1994) VUM5 neuron (Giniger et al., 1994) VUM6 neuron (Giniger et al., 1994)
Detailed Description
Statement Reference Females containing homozygous germline clones show defects in stage 14 egg chambers, including dumpless egg chambers (egg chambers in which the nurse cells have failed to transfer their cytoplasmic contents to the oocyte, have intact nuclei and do not appear to be undergoing programmed cell death) and egg chambers with persisting nurse cell nuclei (egg chambers in which nurse cells have transferred their cytoplasmic contents to the oocyte, but their nuclei have failed to undergo programmed cell death including nuclear condensation). (Paige Bass et al., 2007) In mutant embryos, the motor axons that project through the SNb peripheral nerve fail to form connections to their cognate muscles. Most commonly, SNb axons appear to "stall" somewhere between the point at which they would normally separate from the ISN and the muscle 6/13 junction. In other hemisegments, SNb axons project through the muscles field but fail to branch into the intermuscle clefts where synapses should be formed. The phenotype is slightly stronger than seen in lola^1A4. (Madden et al., 1999) In the embryonic CNS, longitudinal connectives are interrupted and commissures fuse. In some segments the VUM neurons are born and differentiate, but fail to form a well-defined posterior root to the anterior peripheral fascicle. Intersegmental nerve shows various aberrations, failing to grow or wandering into adjacent segments, or defasciculation of ISN sensory axons. The lateral chordotonal organs are sometimes displaced dorsally from their wild type position. There is no segment specificity of these phenotypes. (Giniger et al., 1994)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	28266 y¹ w^*; P{neoFRT}42D P{lacW}lola^5D2/CyO
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	l(2)5D2 (Giniger et al., 1994) P{lacW}lola^5D2 (Giniger et al., 1994, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Paige Bass et al., 2007, Dev. Biol.: The axon guidance gene lola is required for programmed cell death in the Drosophila ovary. [FBrf0194763] Spletter et al., 2007, Neural Dev. 2: 14 Lola regulates Drosophila olfactory projection neuron identity and targeting specificity. [FBrf0208891] Madden et al., 1999, Dev. Biol. 213(2): 301--313 lola has the properties of a master regulator of axon-target interaction for SNb motor axons of Drosophila. [FBrf0111420] Cavarec et al., 1997, Molec. Cell. Biol. 17(1): 482--494 Molecular cloning and characterization of a transcription factor for the copia retrotransposon with homology to the BTB-containing Lola neurogenic factor. [FBrf0091372] Giniger et al., 1994, Development 120(6): 1385--1398 lola encodes a putative transcription factor required for axon growth and guidance in Drosophila. [FBrf0073173] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Horiuchi et al., 2003, Genes Dev. 17(20): Supplemental material. [FBrf0173085]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{lacW}lola5D2 Insertion

Dmel\P{lacW}lola^5D2 Insertion