A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}blw1 Insertion

General Information
Symbol Dmel\P{PZ}blw1 Species D. melanogaster
Name FlyBase ID FBti0003427
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) blw, Ecol\lacZ Viability / fertility
Causes allele(s) blw1, Ecol\lacZblw-1 Stock availability 2 publicly available
LINE ID ms(2)01504
Genomic Location
Chromosomal location 2R ( 59B2 ) Sequence location 2R:18,686,611..18,686,618 [+]
Map ( GBrowse )
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:18,686,611..18,686,618 [+]
(Jacobs et al., 1998, BDGP Project Members, 1994-1999)
2R:18,686,619..18,686,619 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
59B2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
58F5-58F7 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026401
 
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
blw
(Castrillon et al., 1993, Jacobs et al., 1998, BDGP Project Members, 1994-1999)
Ecol\lacZ
(Castrillon et al., 1993)
hide Alleles and Phenotypes
Causes alleles
blw1
(Castrillon et al., 1993, BDGP Project Members, 1994-1999, Jacobs et al., 1998)
Ecol\lacZblw-1
(Castrillon et al., 1993)
Lethality
References
Sterility
References
male sterile
(Jacobs, 1998.9.29)
male sterile | recessive
(Jacobs et al., 1998, Jacobs, 1998.9.29)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Hemizygotes are male sterile.
(Jacobs et al., 1998)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
imaginal disc  
(Meister and Braun, 1995.10)
embryonic/larval fat body  
(Meister and Braun, 1995.10)
embryonic/larval foregut  
(Meister and Braun, 1995.10)
Malpighian tubule  
(Meister and Braun, 1995.10)
embryonic/larval midgut  
(Meister and Braun, 1995.10)
embryonic/larval hindgut  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
ms(2)01504
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
11756
cn1 P{PZ}blw1/CyO; ry506
Kyoto
101940
cn1 P{PZ}blw1 / CyO ; ry506
hide Linkouts
hide Comments
Location 2R:18306696-18306697 confirmed by FlyBase alignment of dbGSS accession AQ026401 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
insertion associated host repeat
(Jacobs et al., 1998, BDGP Project Members, 1994-1999)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1756
(Meister and Braun, 1995.10)
P{PZ}blw1
(FlyBase, 2005, Castrillon et al., 1993, FlyBase, 1992-, Jacobs et al., 1998, BDGP Project Members, 1994-1999)
P{PZ}blw01504
(FlyBase, 1992-)
P{PZ}ms(2)0150401504
(FlyBase, 1992-)
P{ry+t7.2=PZ}blw1
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000212
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Jacobs et al., 1998, Molec. gen. Genet. 259(4): 383--387
A screen for lethal mutations in the chromosomal region 59AB suggests that bellwether encodes the alpha subunit of the mitochondrial ATP synthase in Drosophila melanogaster. [FBrf0104928]
Castrillon et al., 1993, Genetics 135(2): 489--505
Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster: characterization of male-sterile mutants generated by single P element mutagenesis. [FBrf0064394]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Jacobs, 1998.9.29, A2(2nd)27.
A2(2nd)27. [FBrf0104866]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]