FlyBase Insertion Report: Dmel\P{PZ}cuc[1]

General Information

Symbol

Dmel\P{PZ}cuc¹

Species

D. melanogaster

Name

FlyBase ID

FBti0003458

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

ms(2)05158

Affected gene(s)

cuc, Ecol\lacZ

Viability / fertility

Associated allele(s)

cuc¹, Ecol\lacZ^cuc-1

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 27F4 )

Sequence Location

2L:7,423,946..7,423,946 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:7,423,946..7,423,946 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

27F4 (inferred by FlyBase from sequence location)

Cytological location (reported)

28D1-28D12 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ026410

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

cuc

(Castrillon et al., 1993)

Ecol\lacZ

(Braun et al., 1997, Castrillon et al., 1993)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

cuc¹

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^cuc-1

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

Widespread expression in wandering third instar larva; highest levels in imaginal discs (which discs not specified), hindgut, Malpighian tubules, and salivary gland.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

cuc

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

ms(2)05158

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11765

P{PZ}cuc¹ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:7423946-7423947 confirmed by FlyBase alignment of dbGSS accession AQ026410 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P1765

(Meister and Braun, 1995.10)

P{PZ}cuc¹

(FlyBase, 2005, Castrillon et al., 1993, FlyBase, 1992-)

P{PZ}ms(2)05158⁰⁵¹⁵⁸

(FlyBase, 1992-)

P{ry^+t7.2=PZ}cuc¹

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000220

References (11)

Report Sections

Open Close