A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}SmD3k09540 Insertion

General Information
Symbol Dmel\P{lacW}SmD3k09540 Species D. melanogaster
Name FlyBase ID FBti0003686
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Oda, SmD3 Viability / fertility
Causes allele(s) Ecol\lacZSmD3-k09540, Odak09540, SmD3k09540 Stock availability none publicly available
LINE ID l(2)k09540
Genomic Location
Chromosomal location 2R ( 48E4 ) Sequence location
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
48E4 ( near gene of known cytology )
Cytological location
(reported)
48E-48E (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(Kania et al., 1995, Salzberg et al., 1996)
Oda
(Kania et al., 1995)
SmD3
(Schenkel et al., 1999, Schenkel et al., 2002, Kania et al., 1995, Salzberg et al., 1996)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZSmD3-k09540
(Kania et al., 1995, Salzberg et al., 1996)
Odak09540
(Kania et al., 1995, BDGP Project Members, 1994-1999, Schenkel et al., 2002, Salzberg et al., 1996)
SmD3k09540
(Schenkel et al., 1999, Schenkel et al., 2002, Kania et al., 1995, Salzberg et al., 1996)
Lethality
References
lethal | embryonic stage
(Schenkel et al., 2002)
lethal | embryonic stage | recessive
(Schenkel et al., 2002, Kania et al., 1995, Salzberg et al., 1996)
lethal | larval stage
(Schenkel et al., 2002)
Sterility
References
hide Phenotype Manifest In
chordotonal organ
(Schenkel et al., 2002)
embryonic/larval somatic muscle
(Salzberg et al., 1996)
embryonic visceral muscle
(Salzberg et al., 1996)
presumptive embryonic/larval central nervous system
(Salzberg et al., 1996)
presumptive embryonic/larval peripheral nervous system
(Kania et al., 1995, Salzberg et al., 1996)
ventral nerve cord
(Schenkel et al., 2002)
hide Detailed Description
Statement
Reference
Mutant embryos show defects in the lateral chordotonal organs and the ventral nerve cord.
(Schenkel et al., 2002)
Homozygous embryos exhibit loss of neurons.
(Kania et al., 1995)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage | late  
embryonic/larval midgut  
(Kania et al., 1995)
embryonic/larval hindgut  
(Kania et al., 1995)
embryonic stage  
embryonic/larval midgut  
(Salzberg et al., 1996)
embryonic stage 14 -- 17  
embryonic/larval somatic muscle  
(Salzberg et al., 1996)
Additional Information
Statement
Reference
Strong enhancer trap staining is observed in the midgut. Very weak expression is observed in somatic muscle starting in stage 14.
(Salzberg et al., 1996)
In embryo, expressed in hindgut and midgut; strongest expression in the midgut.
(Kania et al., 1995)
Marker for
Reflects
expression of
Oda
(Salzberg et al., 1996)
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k09540
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
P{lacW}gufk09512 and P{lacW}gufk09540 may be members of a cluster, and not unique insertion events.
(Kania et al., 1995)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
l(2)k09540
(FlyBase, 1992-)
P{lacW}gufk09540
(Kania et al., 1995, FlyBase, 1992-)
P{lacW}SmD3k09540
(FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Schenkel et al., 2002, Genetics 161(2): 763--772
P elements inserted in the vicinity of or within the Drosophila snRNP SmD3 gene nested in the first intron of the Ornithine decarboxylase antizyme gene affect only the expression of SmD3. [FBrf0149027]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Salzberg et al., 1996, Genetics 144(1): 183--196
gutfeeling, a Drosophila gene encoding an antizyme-like protein, is required for late differentiation of neurons and muscles. [FBrf0089808]
Kania et al., 1995, Genetics 139(4): 1663--1678
P-element mutations affecting embryonic peripheral nervous system development in Drosophila melanogaster. [FBrf0080145]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract
Schenkel et al., 1999, Europ. Dros. Res. Conf. 16: 214
Down-regulation of the expression of the spliceosome core protein SmD3 gene results in tissue overgrowth. [FBrf0110736]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]