Dmel\P{PZ}spen⁰³³⁵⁰ Insertion

General Information
Symbol	Dmel\P{PZ}spen⁰³³⁵⁰	Species	D. melanogaster
Name		FlyBase ID	FBti0003918
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, spen	Viability / fertility
Causes allele(s)	Ecol\lacZ^spen-03350, spen⁰³³⁵⁰	Stock availability	1 publicly available
LINE ID	l(2)03350
Genomic Location
Chromosomal location	2L ( 21B4 )	Sequence location	2L:161,799..161,799 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:161,799..161,799 [+]
Orientation
Cytological location (computed by FlyBase)	21B4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	21B4-21B6 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	G00736 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Montell et al., 1992, Vosshall, 1998.8.10) spen (Montell et al., 1992)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^spen-03350 (Montell et al., 1992, Vosshall, 1998.8.10) spen⁰³³⁵⁰ (BDGP Project Members, 1994-1999, Kuang et al., 2000, Wiellette et al., 1999, Lane et al., 2000, Braun et al., 1997, Spradling et al., 1999)

Lethality References lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999, Kuang et al., 2000)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage embryonic/larval foregut (Meister and Braun, 1995.10) embryonic/larval fat body (Meister and Braun, 1995.10) embryonic/larval midgut (Meister and Braun, 1995.10) Malpighian tubule (Meister and Braun, 1995.10) embryonic/larval hindgut (Meister and Braun, 1995.10) embryonic/larval brain (Meister and Braun, 1995.10) imaginal disc (Meister and Braun, 1995.10) ring gland (Meister and Braun, 1995.10) embryonic/larval salivary gland (Meister and Braun, 1995.10) adult stage adult brain (Vosshall, 1998.8.10)
Additional Information
Statement Reference In adult, expression throughout brain. (Vosshall, 1998.8.10) In wandering third instar larva, high levels of expression in imaginal discs (which discs not specified), throughout gut, and in salivary glands; slightly lower levels of expression in Malpighian tubules and fat body; weak expression in brain and ring gland. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)03350 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11295 P{PZ}spen⁰³³⁵⁰ cn¹/CyO; ry⁵⁰⁶
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1295 (Meister and Braun, 1995.10) P{PZ}l(2)03350⁰³³⁵⁰ (Montell et al., 1992, FlyBase, 1992-) P{PZ}spen⁰³³⁵⁰ (FlyBase, 1992-) P{ry^+t7.2=PZ}l(2)03350⁰³³⁵⁰ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000316
References ( 13 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Kuang et al., 2000, Development 127(7): 1517--1529 split ends encodes large nuclear proteins that regulate neuronal cell fate and axon extension in the Drosophila embryo. [FBrf0126772] Lane et al., 2000, Genetics 155(1): 233--244 A screen for modifiers of Cyclin E function in Drosophila melanogaster identifies Cdk2 mutations, revealing the insignificance of putative phosphorylation sites in Cdk2. [FBrf0127183] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Wiellette et al., 1999, Development 126(23): 5373--5385 spen encodes an RNP motif protein that interacts with Hox pathways to repress the development of head-like sclerites in the Drosophila trunk. [FBrf0112078] Braun et al., 1997, Genetics 147(2): 623--634 Drosophila immunity: analysis of larval hemocytes by P-element-mediated enhancer trap. [FBrf0098749] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714] Montell et al., 1992, Cell 71: 51--62 slow border cells, a locus required for a developmentally regulated cell migration during oogenesis, encodes Drosophila C/EBP. [FBrf0055567]
Personal communication to FlyBase	Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}spen03350 Insertion

Dmel\P{PZ}spen⁰³³⁵⁰ Insertion