FlyBase Insertion Report: Dmel\P{PZ}poe[03420]

General Information

Symbol

Dmel\P{PZ}poe⁰³⁴²⁰

Species

D. melanogaster

Name

FlyBase ID

FBti0003925

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

ms(2)03420

l(2)03420

Affected gene(s)

Ecol\lacZ, poe

Viability / fertility

Associated allele(s)

Ecol\lacZ^poe-03420, poe⁰³⁴²⁰

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 28E1 )

Sequence Location

2L:8,071,599..8,071,599 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:8,071,599..8,071,599 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

28E1 (inferred by FlyBase from sequence location)

Cytological location (reported)

27C1-27C9 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2L:8071600..8071608 .

Sequence Data

Flanking sequence

AQ026406

(FlyBase, 1992-)

AF096896

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Meister and Braun, 1995.10, Carlson, 1993, Castrillon et al., 1993, Riesgo-Escovar et al., 1992)

poe

(Meister and Braun, 1995.10, Castrillon et al., 1993)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^poe-03420

Phenotypic Class Phenotype Manifest in Detailed Description

poe⁰³⁴²⁰

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval antenno-maxillary sensory complex

(Riesgo-Escovar et al., 1992)

adult stage

maxillary palp

(Riesgo-Escovar et al., 1992)

second segment of antenna

(Riesgo-Escovar et al., 1992)

adult stage | female

female reproductive system

(Riesgo-Escovar et al., 1992)

central nervous system

(Riesgo-Escovar et al., 1992)

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

gonad

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

adult stage | male

male reproductive system

(Riesgo-Escovar et al., 1992)

adult stage

labellum

(Riesgo-Escovar et al., 1992)

basiconic sensillum of antennal segment 3

(Riesgo-Escovar et al., 1992)

Johnston organ

(Riesgo-Escovar et al., 1992)

wing vein L1

(Riesgo-Escovar et al., 1992)

Additional Information

Statement

Reference

In wandering third instar, low level of expression in larval brain and imaginal discs; higher level in gonad. (Not specified which imaginal discs.)

(Meister and Braun, 1995.10)

Enhancer trap expression occurs in thedorso-medial portion of the third antennal segment in a pattern similar tothat of the sensilla basiconica. One or a few cells in the second antennalsegment are stained that may be part of Johnston's organ. Staining is alsoobserved on the anterior margin of the wing, in the CNS, and inreproductive tissue.

(Riesgo-Escovar et al., 1992)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

ms(2)03420

(Gene Disruption Project members, 2001-)

l(2)03420

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11761

P{PZ}poe⁰³⁴²⁰ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:8071599-8071600 confirmed by FlyBase alignment of dbGSS accession AQ026406 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

May be multiple insertion line: in situ data (27C1-27C9) conflict with location inferred from flanking sequence (28E1).

(FlyBase, 1992-)

8 bp insertion associated host repeat

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

P1761

(Meister and Braun, 1995.10)

P{PZ}ms(2)27C⁰³⁴²⁰

(FlyBase, 1992-)

P{PZ}ms(2)ry27C

(FlyBase, 1992-)

P{PZ}poe⁰³⁴²⁰

(FlyBase, 2005, FlyBase, 1992-)

P{PZ}push⁰³⁴²⁰

(Castrillon et al., 1993, FlyBase, 1992-)

P{ry^+t7.2=PZ}ms(2)27C⁰³⁴²⁰

(FlyBase, 1992-)

l(2)03420

(FlyBase, 1992-)

push3420

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000694

References (15)

Report Sections

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