Dmel\P{lacW}RpS13¹ Insertion

General Information
Symbol	Dmel\P{lacW}RpS13¹	Species	D. melanogaster
Name		FlyBase ID	FBti0003942
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	RpS13	Viability / fertility
Causes allele(s)	RpS13¹	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2L ( 29B2 )	Sequence location	2L:8,364,578..8,364,578 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:8,364,578..8,364,578 [-] (Saeboe-Larssen and Lambertsson, 1996)
Orientation
Cytological location (computed by FlyBase)	29B2 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	RpS13 (Saeboe-Larssen and Lambertsson, 1996, Oren-Giladi et al., 2008, Saeboe-Larssen and Lambertsson, 1996)
Alleles and Phenotypes
Causes alleles	RpS13¹ (Saeboe-Larssen and Lambertsson, 1996, Oren-Giladi et al., 2008)

Lethality References lethal \| recessive (Saeboe-Larssen and Lambertsson, 1996)
Sterility References
Phenotype Manifest In
	macrochaeta (Saeboe-Larssen and Lambertsson, 1996) scutellar bristle (Marygold et al., 2007)
Detailed Description
Statement Reference RpS13[1]/+ flies have shorter and thinner scutellar bristles. (Marygold et al., 2007) Homozygous mutant RpI135^k16513 clone survival can be rescued by genetically reducing the growth of surrounding cells by making them heterozygous for a dominant Minute RpS13¹ mutant. In this case, RpI135^k16513 mutant clones are recovered in all RpS13¹ heterozygous discs that are examined at 96hrs after clone induction, a time-point at which all clones are normally eliminated in a wild-type background. However, these clones colonise a significantly smaller area of RpS13¹/+ discs compared with wild-type clones (28.8% compared to 67.8% of the total disc area), indicating that they are still growth impaired. Compared to surrounding heterozygote RpS13¹ or wild-type cells, RpI135^k16513 mutant clone cells exhibit no significant change in either cell size or cell-cycle phasing. (Grewal et al., 2005) Minute phenotype. (Saeboe-Larssen and Lambertsson, 1996)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	2246 y¹ w^*; P{lacW}RpS13¹/CyO
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}M(2)32A¹ (FlyBase, 1992-) P{lacW}RpS13¹ (FlyBase, 1992-, Saeboe-Larssen and Lambertsson, 1996, Oren-Giladi et al., 2008) P{w^+mC=lacW}M(2)32A¹ (FlyBase, 1992-) P{w^+mC=lacW}RpS13¹ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0001353 FBti0002821
References ( 6 )
Research paper	Oren-Giladi et al., 2008, Genes Cells 13(3): 221--231 Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development. [FBrf0203123] Marygold et al., 2007, Genome Biol. 8(10): R216 The ribosomal protein genes and Minute loci of Drosophila melanogaster. [FBrf0205398] Grewal et al., 2005, Nature Cell Biol. 7(3): 295--302 Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development. [FBrf0184182] Saeboe-Larssen and Lambertsson, 1996, Genetics 143(2): 877--885 A novel Drosophila minute locus encodes ribosomal protein S13. [FBrf0087682] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{lacW}RpS131 Insertion

Dmel\P{lacW}RpS13¹ Insertion