A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}blp01618 Insertion

General Information
Symbol Dmel\P{PZ}blp01618 Species D. melanogaster
Name FlyBase ID FBti0003977
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) blp, Ecol\lacZ Viability / fertility
Causes allele(s) blp01618, Ecol\lacZblp-01618 Stock availability 1 publicly available
LINE ID l(3)01618
Genomic Location
Chromosomal location 3R ( 89A8 ) Sequence location 3R:11,759,635..11,759,635 [+]
Map ( GBrowse ) detailed view FBti0075284 FBti0077663 FBti0045626 FBti0130184 FBti0110613 FBti0003977 FBti0039500 FBti0126639 FBti0044035
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:11,759,635..11,759,635 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
89A8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
89A8-89A9 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026173
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
blp
(Hacker et al., 1995)
Ecol\lacZ
(Hacker et al., 1995, Becker et al., 2001, Vosshall, 1998.8.10)
hide Alleles and Phenotypes
Causes alleles
blp01618
(BDGP Project Members, 1994-1999, Becker et al., 2001, Spradling et al., 1999)
Ecol\lacZblp-01618
(Hacker et al., 1995, Becker et al., 2001, Vosshall, 1998.8.10)
Lethality
References
lethal | embryonic stage | rescuable maternal effect
(Perrimon et al., 1996)
lethal | larval stage | recessive
(Perrimon et al., 1996)
lethal | recessive
(Spradling et al., 1999)
semi-lethal
 
semi-lethal | recessive
(Becker et al., 2001, Berkeley Drosophila Genome Project, 1993.6.1)
Sterility
References
hide Phenotype Manifest In
embryonic/first instar larval cuticle
(Perrimon et al., 1996)
melanotic mass
(Becker et al., 2001)
segment
(Perrimon et al., 1996)
hide Detailed Description
Statement
Reference
Some homozygous escapers eclose at 25oC. On average they require 5.5-6 days longer to develop than normal. At 18oC eclosion of escapers is delayed by 14 days compared to wild type. Heterozygotes show no obvious phenotype. At the transition from second to third larval instar stage, homozygotes develop pigment encapsulated cell clusters, which gradually lose their cellular appearance and transform into dark brown to black inclusion bodies of variable size ("black pearls"). These melanotic "tumours" are found at arbitrary sites apparently without contact to the surrounding tissue. In surviving adults the tumours are located predominantly in the abdomen and less frequently in the thorax. The tumours stop growing in the adults and appear to be benign without obvious effects on longevity.
(Becker et al., 2001)
Germline clones produce eggs with patterning defects: variable segmentation defects.
(Perrimon et al., 1996)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 15 -- 17  
antenno-maxillary complex | restricted  
(Becker et al., 2001)
Comment:localized to small cell clusters
embryonic/larval brain | restricted  
(Becker et al., 2001)
Comment:localized to small cell clusters
ventral midline  
(Becker et al., 2001)
Comment:localized to small cell clusters
second instar larval stage  
embryonic/larval brain | restricted  
(Becker et al., 2001)
Comment:localized to a very few small cell clusters
ventral nerve cord  
(Becker et al., 2001)
Comment:localized to a very few small cell clusters
third instar larval stage  
embryonic/larval brain | restricted  
(Becker et al., 2001)
Comment:localized to a many small cell clusters; excludes optic lobes
ventral nerve cord  
(Becker et al., 2001)
Comment:localized to a many cell clusters
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)01618
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11539
ry506 P{PZ}blp01618/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3R:11759635-11759636 confirmed by FlyBase alignment of dbGSS accession AQ026173 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{PZ}blp01618
(FlyBase, 2005, FlyBase, 1992-)
P{PZ}l(3)0161801618
(Hacker et al., 1995, FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)0161801618
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000496
hide References ( 13 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Becker et al., 2001, Gene 262(1-2): 15--22
The black-pearl gene of Drosophila defines a novel conserved protein family and is required for larval growth and survival. [FBrf0134627]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Perrimon et al., 1996, Genetics 144(4): 1681--1692
Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142]
Hacker et al., 1995, EMBO J. 14(21): 5306--5317
The Drosophila fork head domain protein crocodile is required for the establishment of head structures. [FBrf0085156]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
Berkeley Drosophila Genome Project, 1993.6.1, Insertion alleles.
Insertion alleles. [FBrf0122635]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]