A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}CrebA03576 Insertion

General Information
Symbol Dmel\P{PZ}CrebA03576 Species D. melanogaster
Name FlyBase ID FBti0004124
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) CrebA, Ecol\lacZ Viability / fertility
Causes allele(s) CrebA03576, Ecol\lacZCrebA-03576 Stock availability 1 publicly available
LINE ID l(3)03576
Genomic Location
Chromosomal location 3L ( 71E1 ) Sequence location 3L:15,537,388..15,537,388 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0061447 FBti0061450 FBti0069135 FBti0100026 FBti0055738 FBti0069144 FBti0070778 FBti0068224 FBti0052248 FBti0132620 FBti0004124 FBti0052789 FBti0057949 FBti0050194 FBti0151940 FBti0011562 FBti0110650 FBti0108132 FBti0103482 FBti0039187 FBti0035137
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:15,537,388..15,537,388 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
71E1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
71D1--71D2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034057
(FlyBase, 1992-,  
G00569
(FlyBase, 1992-)
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CrebA
(Andrew et al., 1997, Tsurudome et al., 2010)
Ecol\lacZ
(Andrew et al., 1997)
hide Alleles and Phenotypes
Causes alleles
CrebA03576
(Andrew et al., 1997, Spradling et al., 1999, BDGP Project Members, 1994-1999, Meyer et al., 2006, Tsurudome et al., 2010)
Ecol\lacZCrebA-03576
(Andrew et al., 1997)
Lethality
References
lethal | larval stage | recessive
(Andrew et al., 1997)
lethal | recessive
(Spradling et al., 1999, Rose et al., 1997)
partially lethal - majority die
 
Sterility
References
hide Phenotype Manifest In
denticle belt | larval stage
(Andrew et al., 1997)
embryonic/larval cuticle
(Andrew et al., 1997)
larva
(Andrew et al., 1997)
hide Detailed Description
Statement
Reference
Heterozygosity for CrebA[03576] has no effect on the evoked excitatory junctional potentials (EJPs) and quantal content (QC) recorded from muscles of homozygous Df(2R)KT40 mutant larvae.
(Tsurudome et al., 2010)
Homozygous larvae are 40% the length and the same width as their heterozygous siblings, length differences arise during larva formation as embryos are about the same length as their heterozygous siblings. The larval cuticle of homozygotes is weak, as seen by frequent holes at random positions in the exoskeleton. The dorsal and ventral larval cuticle is composed of pattern elements found in the ventrolateral position in the wild-type larval cuticle.
(Andrew et al., 1997)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- third instar larval stage  
salivary gland  
(Rose et al., 1997)
embryonic stage 15 -- 16  
stomodeum  
(Rose et al., 1997)
posterior spiracle primordium | surrounding  
(Rose et al., 1997)
adult stage  
spermathecum  
(Rose et al., 1997)
oogenesis stage S9 -- S10 & adult stage | female  
follicle cell  
(Rose et al., 1997)
Additional Information
Statement
Reference
Marker for
embryonic posterior spiracle | surrounding
(Rose et al., 1997)
follicle cell
(Rose et al., 1997)
follicle cell salivary gland stomodeum embryonic posterior spiracle | surrounding spermatecum
(Rose et al., 1997)
salivary gland
(Rose et al., 1997)
spermatecum
(Rose et al., 1997)
stomodeum
(Rose et al., 1997)
Reflects
expression of
CrebA
(Rose et al., 1997)
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)03576
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
10183
P{PZ}CrebA03576 ry506/TM3, P{ftz/lacC}SC1, ryRK Sb1 Ser1
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{PZ}CrebA03576
(Andrew et al., 1997, FlyBase, 1992-, Meyer et al., 2006, )
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Tsurudome et al., 2010, Neuron 68(5): 879--893
The Drosophila miR-310 Cluster Negatively Regulates Synaptic Strength at the Neuromuscular Junction. [FBrf0212495]
Meyer et al., 2006, PLoS Genet. 2(8): e134
Overlapping functions of argonaute proteins in patterning and morphogenesis of Drosophila embryos. [FBrf0194224]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Andrew et al., 1997, Development 124(1): 181--193
The Drosophila dCREB-A gene is required for dorsal/ventral patterning of the larval cuticle. [FBrf0091006]
Rose et al., 1997, Genetics 146(2): 595--606
The CRE-binding protein dCREB-A is required for Drosophila embryonic development. [FBrf0093681]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]