A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}edlk06602 Insertion

General Information
Symbol Dmel\P{lacW}edlk06602 Species D. melanogaster
Name FlyBase ID FBti0004129
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, edl Viability / fertility
Causes allele(s) Ecol\lacZedl-k06602, edlk06602 Stock availability 2 publicly available
LINE ID l(2)k06602
Genomic Location
Chromosomal location 2R ( 55E6 ) Sequence location 2R:14,561,159..14,561,159 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0070862 FBti0025912 FBti0006735 FBti0075437 FBti0034771 FBti0100156 FBti0075044 FBti0102557 FBti0104533 FBti0004129 FBti0043545 FBti0075369
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:14,561,159..14,561,159 [-]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
55E6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
55E6-55E9 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025800
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Baker et al., 2001)
edl
(Beaton, 1997.3.4)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZedl-k06602
(BDGP Project Members, 1994-1999, Baker et al., 2001)
edlk06602
(Beaton, 1997.3.4, BDGP Project Members, 1994-1999, Bianchi-Frias et al., 2004, Vivekanand et al., 2004, Baker et al., 2001, Roch et al., 1998, Spradling et al., 1999)
Lethality
References
lethal | recessive
(Roch et al., 1998, Spradling et al., 1999, Baker et al., 2001, Beaton, 1997.3.4)
Sterility
References
hide Phenotype Manifest In
denticle belt & embryonic abdominal segment 1
(Baker et al., 2001)
denticle belt & embryonic abdominal segment 2
(Baker et al., 2001)
denticle belt & embryonic abdominal segment 3
(Baker et al., 2001)
denticle belt & embryonic abdominal segment 4
(Baker et al., 2001)
embryonic ventral epidermis & embryonic abdominal segment 1
(Baker et al., 2001)
embryonic ventral epidermis & embryonic abdominal segment 2
(Baker et al., 2001)
embryonic ventral epidermis & embryonic abdominal segment 3
(Baker et al., 2001)
embryonic ventral epidermis & embryonic abdominal segment 4
(Baker et al., 2001)
presumptive embryonic/larval central nervous system
(Tootle et al., 2003)
hide Detailed Description
Statement
Reference
The rough eye phenotype caused by aopACT.sev is enhanced if the flies also carry edlk06602/+.
(Vivekanand et al., 2004)
Neuronal differentiation in the central nervous system is inhibited in homozygous embryos.
(Tootle et al., 2003)
Denticle belts are narrowed along the midline, in abdominal segments 1 to 4. This is reminiscent of phenotypes shown by rho and pnt mutant embryos.
(Baker et al., 2001)
Lethality occurs during third instar larval or pupal stages. Mutants do not show any visible disc abnormalities.
(Roch et al., 1998)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
<e> is <t> E | stage 6-9 <a> ventral neurogenic region <s><note>
(Baker et al., 2001)
<e> is <t> E | stage 9-11 <a> ventral midline <s><note>
(Baker et al., 2001)
<e> is <t> E | stage 6-9 <a> procephalic neurogenic region <s><note>
(Baker et al., 2001)
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k06602
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10633
y1 w67c23; P{lacW}edlk06602/CyO
Kyoto
102488
y1 w67c23 ; P{lacW}edlk06602 / CyO
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hide Comments
Location 2R:14188516-14188517 determined by FlyBase alignment of dbGSS accession AQ025800 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}edlk06602
(FlyBase, 2005, FlyBase, 1992-, )
P{lacW}l(2)k06602k06602
(Beaton, 1997.3.4, FlyBase, 1992-)
P{lacW}maek06602
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 14 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Bianchi-Frias et al., 2004, PLoS Biol. 2(7): e178
Hairy transcriptional repression targets and cofactor recruitment in Drosophila. [FBrf0179136]
Vivekanand et al., 2004, Mech. Dev. 121(12): 1469--1479
MAE, a dual regulator of the EGFR signaling pathway, is a target of the Ets transcription factors PNT and YAN. [FBrf0180485]
Tootle et al., 2003, Development 130(5): 845--857
CRM1-mediated nuclear export and regulated activity of the Receptor Tyrosine Kinase antagonist YAN require specific interactions with MAE. [FBrf0157239]
Baker et al., 2001, Nature 411(6835): 330--334
Mae mediates MAP kinase phosphorylation of Ets transcription factors in Drosophila. [FBrf0135901]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Roch et al., 1998, Mol. Gen. Genet. 257(2): 103--112
Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Beaton, 1997.3.4, Update: Deficiencies.
Update: Deficiencies. [FBrf0092781]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]