Comment: strong expression
Comment: 54 of 103 labeled neurons
Comment: 62 of 110 labeled neurons
Drives expression in the mushroom body and mushroom body neurons.
Scer\GAL4ey-OK107 drives expression in neuroblasts, ganglion mother cells and neurons of the developing larval medulla.
Scer\GAL4ey-OK107 drives expression in neurons that densely innervate the larval antennal lobes (AL). The cell bodies of labeled neurons are located in a cluster ventrolateral to the AL.
The expression of ey, ap, and Dll were compared in outer optic lobes (OPC) starting in late third instar larvae. At this stage they were expressed as three distinct cell populations. In anterior sections, the three genes are expressed a three parallele stripes of cells that represent rows of neurons that emerge from the OPC. They correspond to progeny from the youngest to oldest neuroblasts. In middle sections, Dll-positive cells are generated in the progeny of the oldest neuroblasts, with ey-positive and ap-positive cells often placed below Dll-positive (in cells that had emerged earlier from the these neuroblasts). By the beginning of pupation, the number of cells origination from the OPC increased. A major reorganization of optic lobe structure occurs around P20 such that the three stripes are no longer distinguishable and the three cell populations are extensively interspersed within the adult medulla cortex. ey is expressed in neuroblasts and in the vast majority of neurons derived from ey-positive neuroblasts. Expression does not persist in pupae.
Scer\GAL4ey-OK107 is expressed in the larval and adult mushroom body alpha'/beta', alpha/beta and gamma neurons and the Kenyon cells in the calyx. Co-expression with presynaptic markers labels restricted domains in the dendrites of Kenyon cells in the mushroom body lobes and calyx. A postsynaptic marker is expressed in the dendrites of Kenyon cells in the calyx, in a distinct domain to the pre-synapses.
Expression of Scer\GAL4ey-OK107 is seen in cell bodies found in the local interneuron cluster located ventral to the antennal lobe. Most labelled cells are bilaterally projecting interneurons.
Scer\GAL4ey-OK107 drives expression in the insulin producing cells (IPC) and in other cells of the dorsomedial neurosecretory cell cluster. Scer\GAL4ey-OK107 co-localises with Ilp2 protein in the IPC cells.
Scer\GAL4ey-OK107 drives expression in GABAergic and glutamatergic antennal lobe local interneurons, and in mushroom body neurons.
Expression is observed in the alpha, beta, gamma, alpha', and beta' neurons of the adult mushroom body.
Scer\GAL4ey-OK107 drives expression in 30% of the cells in the medulla cortex, whose processes are preferentially located in the lower medulla layers. Cells labeled with Scer\GAL4ey-OK107 do not overlap with cells expressing Ecol\lacZap-rK568 or Scer\GAL4Dll-md23.