Dmel\P{PZ}salm⁰³⁶⁰² Insertion

General Information
Symbol	Dmel\P{PZ}salm⁰³⁶⁰²	Species	D. melanogaster
Name		FlyBase ID	FBti0004231
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, salm	Viability / fertility
Causes allele(s)	Ecol\lacZ^salm-03602, salm⁰³⁶⁰²	Stock availability	1 publicly available
LINE ID	l(2)03602
Genomic Location
Chromosomal location	2L ( 32F2 )	Sequence location	2L:11,445,759..11,445,759 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:11,445,759..11,445,759 [+]
Orientation
Cytological location (computed by FlyBase)	32F2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	32F1-32F2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location	Mapped to the region 2L:11450129..11454221 (EcoRI restriction fragment); r5 coordinates. The orientation of the insertion was not reported. (Barrio et al., 1999)
Sequence Data
Flanking sequence	G00737 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Treisman and Rubin, 1996, Dong et al., 2000, Chu et al., 2002, Cook et al., 2004, Adachi-Yamada and O'Connor, 2002, Zelhof et al., 1997, Harvie et al., 1998, Vosshall, 1998.8.10, Beckervordersandforth et al., 2008) salm (Treisman and Rubin, 1996, Barrio et al., 1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^salm-03602 (Treisman and Rubin, 1996, Dong et al., 2000, Chu et al., 2002, Cook et al., 2004, Adachi-Yamada and O'Connor, 2002, Zelhof et al., 1997, Harvie et al., 1998, Vosshall, 1998.8.10, Beckervordersandforth et al., 2008) salm⁰³⁶⁰² (Treisman and Rubin, 1996, BDGP Project Members, 1994-1999, Cook et al., 2003, Spradling et al., 1999, Barrio et al., 1999, Mesilaty-Gross et al., 1999, Harvie et al., 1998)

Lethality References lethal \| recessive (Treisman and Rubin, 1996, Harvie et al., 1998, Spradling et al., 1999) partially lethal - majority die partially lethal - majority die \| recessive (Barrio et al., 1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Hemizygotes have greatly reduced viability (6% in salm⁰³⁶⁰²/Df(2L)Prl and 24% in salm⁰³⁶⁰²/Df(2L)esc-P3-0 flies compared to control heterozygous siblings) and delayed eclosion. (Barrio et al., 1999)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference larval stage prothoracic gland (Harvie et al., 1998) third instar larval stage photoreceptor cell \| presumptive (Treisman and Rubin, 1996) eye disc (Treisman and Rubin, 1996) wandering third instar larval stage embryonic/larval oenocyte (Meister and Braun, 1995.10) imaginal disc (Harvie et al., 1998, Meister and Braun, 1995.10) embryonic/larval optic lobe (Harvie et al., 1998) ventral nerve cord (Harvie et al., 1998)
Additional Information
Statement Reference During all larval stages, expressed in lateral secretory cells (prothoracic gland) of the ring gland. (Harvie et al., 1998) In third instar eye disc, expressed in all photoreceptors. (Treisman and Rubin, 1996)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)03602 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11340 P{PZ}salm⁰³⁶⁰² cn¹/CyO; ry⁵⁰⁶
Linkouts
Comments
	The insertion site maps to a 4kb EcoRI fragment; the orientation of the insertion was not reported. (Barrio et al., 1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1340 (Meister and Braun, 1995.10) P{PZ}l(2)03602⁰³⁶⁰² (FlyBase, 1992-) P{PZ}salm⁰³⁶⁰² (Treisman and Rubin, 1996, FlyBase, 1992-, Barrio et al., 1999, ) P{ry^+t7.2=PZ}l(2)03602⁰³⁶⁰² (FlyBase, 1992-) P{ry^+t7.2=PZ}salm⁰³⁶⁰² (Cook et al., 2004) sal-lacZ (Cook et al., 2004, Adachi-Yamada and O'Connor, 2002, Chu et al., 2002, Dong et al., 2000) spalt-major-lacZ (Beckervordersandforth et al., 2008)
Secondary FlyBase IDs
	FBti0000325
References ( 20 )
Research paper	Beckervordersandforth et al., 2008, Mech. Dev. 125(5-6): 542--557 Subtypes of glial cells in the Drosophila embryonic ventral nerve cord as related to lineage and gene expression. [FBrf0205499] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Cook et al., 2004, Development 131(9): 2113--2124 brinker and optomotor-blind act coordinately to initiate development of the L5 wing vein primordium in Drosophila. [FBrf0174576] Cook et al., 2003, Dev. Cell 4(6): 853--864 Distinction between color photoreceptor cell fates is controlled by Prospero in Drosophila. [FBrf0160456] Adachi-Yamada and O'Connor, 2002, Dev. Biol. 251(1): 74--90 Morphogenetic apoptosis: a mechanism for correcting discontinuities in morphogen gradients. [FBrf0151891] Chu et al., 2002, Development 129(3): 695--704 Limb type-specific regulation of bric a brac contributes to morphological diversity. [FBrf0144840] Dong et al., 2000, Development 127(2): 209--216 Coexpression of the homeobox genes Distal-less and homothorax determines Drosophila antennal identity. [FBrf0122999] Barrio et al., 1999, Dev. Biol. 215(1): 33--47 Identification of regulatory regions driving the expression of the Drosophila spalt complex at different developmental stages. [FBrf0111797] Mesilaty-Gross et al., 1999, Gene 231(1-2): 173--186 The Drosophila STAM gene homolog is in a tight gene cluster, and its expression correlates to that of the adjacent gene ial. [FBrf0108471] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Harvie et al., 1998, Genetics 149(1): 217--231 Genes expressed in the ring gland, the major endocrine organ of Drosophila melanogaster. [FBrf0102844] Zelhof et al., 1997, Development 124(13): 2499--2506 A role for ultraspiracle, the Drosophila RXR, in morphogenetic furrow movement and photoreceptor cluster formation. [FBrf0097996] Treisman and Rubin, 1996, Mech. Dev. 56(1-2): 17--24 Targets of glass regulation in the Drosophila eye disc. [FBrf0089138] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}salm03602 Insertion

Dmel\P{PZ}salm⁰³⁶⁰² Insertion