Dmel\P{PZ}osp^rJ571 Insertion

General Information
Symbol	Dmel\P{PZ}osp^rJ571	Species	D. melanogaster
Name		FlyBase ID	FBti0004242
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, osp	Viability / fertility
Causes allele(s)	Ecol\lacZ^osp-rJ571, osp^rJ571	Stock availability	1 publicly available
LINE ID	l(2)rJ571
Genomic Location
Chromosomal location	2L ( 35B4 )	Sequence location	2L:14,688,936..14,688,936 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:14,688,936..14,688,936 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	35B4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	35B1-35B4 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026134 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Treisman and Rubin, 1996) osp (Treisman and Rubin, 1996)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^osp-rJ571 (Treisman and Rubin, 1996) osp^rJ571 (Treisman and Rubin, 1996, Spradling, 1999.12.9, BDGP Project Members, 1994-1999, Spradling et al., 1999, Ashburner et al., 1999.10.12, Ashburner et al., 1999, Ashburner et al., 1999)

Lethality References lethal \| recessive (Treisman and Rubin, 1996) semi-lethal (Spradling, 1999.12.9, ) semi-lethal \| recessive (Berkeley Drosophila Genome Project, 1993.6.1)
Sterility References
Phenotype Manifest In
	crystal cell (Milchanowski et al., 2004) wing (Berkeley Drosophila Genome Project, 1993.6.1)
Detailed Description
Statement Reference The average number of crystal cells per embryo is reduced (to 11.8) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average). (Milchanowski et al., 2004) Flies have an osp phenotype. (Ashburner et al., 1999) Homozygotes have bent wings. (Berkeley Drosophila Genome Project, 1993.6.1) visible: wings bent
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference third instar larval stage morphogenetic furrow \| posterior to (Treisman and Rubin, 1996) eye disc (Treisman and Rubin, 1996)
Additional Information
Statement Reference Expressed in all cells of the eye disc, "starting soon after the morphogenetic furrow". (Treisman and Rubin, 1996)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)rJ571 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	12046 P{PZ}osp^rJ571/CyO; ry⁵⁰⁶
Linkouts
Comments
	Location 2L:14688936-14688937 confirmed by FlyBase alignment of dbGSS accession AQ026134 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}l(2)rJ571^rJ571 (FlyBase, 1992-) P{PZ}osp^rJ571 (FlyBase, 2005, Treisman and Rubin, 1996, FlyBase, 1992-) P{PZ}rJ571 (Ashburner et al., 1999) P{ry^+t7.2=PZ}l(2)rJ571^rJ571 (FlyBase, 1992-) P{ry^+t7.2=PZ}osp^rJ571 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000453 FBti0003026
References ( 14 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Milchanowski et al., 2004, Genetics 168(1): 325--339 Identification and characterization of genes involved in embryonic crystal cell formation during Drosophila hematopoiesis. [FBrf0180297] Ashburner et al., 1999, Genetics 153(1): 179--219 An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. [FBrf0111326] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Treisman and Rubin, 1996, Mech. Dev. 56(1-2): 17--24 Targets of glass regulation in the Drosophila eye disc. [FBrf0089138] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material	Ashburner et al., 1999, Genetics 153(1): Table S1. P-element insertions in the Adh region. [FBrf0112150]
Personal communication to FlyBase	Ashburner et al., 1999.10.12, Adh. Adh. [FBrf0112153] Spradling, 1999.12.9, Helping FlyBase with P-element paper. Helping FlyBase with P-element paper. [FBrf0125024] Berkeley Drosophila Genome Project, 1993.6.1, Insertion alleles. Insertion alleles. [FBrf0122635]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}osprJ571 Insertion

Dmel\P{PZ}osp^rJ571 Insertion