Dmel\P{PZ}Hmgcr⁰¹¹⁵² Insertion

General Information
Symbol	Dmel\P{PZ}Hmgcr⁰¹¹⁵²	Species	D. melanogaster
Name		FlyBase ID	FBti0004269
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Hmgcr	Viability / fertility
Causes allele(s)	Ecol\lacZ^Hmgcr-01152, Hmgcr⁰¹¹⁵²	Stock availability	1 publicly available
LINE ID	l(3)01152
Genomic Location
Chromosomal location	3R ( 95B1 )	Sequence location	3R:19,559,215..19,559,215 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	3 (Treisman and Rubin, 1996)
Sequence Location	3R:19,559,215..19,559,215 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	95B1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	95B5-95B6 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026166 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Treisman and Rubin, 1996, Vosshall, 1998.8.10, Russell et al., 1998) Hmgcr (Treisman and Rubin, 1996)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Hmgcr-01152 (Treisman and Rubin, 1996, Vosshall, 1998.8.10, Russell et al., 1998) Hmgcr⁰¹¹⁵² (Treisman and Rubin, 1996, BDGP Project Members, 1994-1999, Van Doren et al., 1998, Russell et al., 1998, Spradling et al., 1999, Yi et al., 2006, Yi, 2006)

Lethality References lethal \| embryonic stage \| partially \| rescuable maternal effect (Perrimon et al., 1996) lethal \| embryonic stage \| recessive (Russell et al., 1998, Perrimon et al., 1996) lethal \| recessive (Treisman and Rubin, 1996, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	cardioblast (Yi et al., 2006) cardioblast \| embryonic stage 17 (Yi, 2006) denticle belt \| rescuable maternal effect (Deshpande and Schedl, 2005) embryonic/first instar larval cuticle (Russell et al., 1998, Perrimon et al., 1996) embryonic/first instar larval cuticle \| rescuable maternal effect (Deshpande and Schedl, 2005) embryonic dorsal vessel (Yi et al., 2006) embryonic pericardial cell (Yi et al., 2006) embryonic pericardial cell \| embryonic stage 17 (Yi, 2006) filzkorper \| rescuable maternal effect (Deshpande and Schedl, 2005) germline cell (Van Doren and Lehmann, 2001.2.14) mouthpart \| embryonic stage \| rescuable maternal effect (Deshpande and Schedl, 2005) pole cell (Santos and Lehmann, 2004)
Detailed Description
Statement Reference Cardioblasts and pericardial cells are properly specified and aligned until stage 16, but in later embryos the cardioblasts and pericardial cells begain to dissociate from each other. (Yi, 2006) Homozygous stage 17 embryos show a "broken hearted" phenotype; the pericardial cells are dissociated from cardioblasts in the dorsal vessel. Hmgcr⁰¹¹⁵²/Df(3R)Exel9013 mutants show distortion of the shape of the dorsal vessel. (Yi et al., 2006) Embryos whose mothers carry gemline clones homozygous for Hmgcr⁰¹¹⁵² and whose fathers are Hmgcr^11.57/+ often have severe cuticle defects including disrupted denticle belts, abnormal mouthparts and incomplete filzkorper, but others have normal cuticles or mild fusion of abdominal segments 8 and 9. This latter group are probably paternally rescued. (Deshpande and Schedl, 2005) About 40% of Fpps^k06103; Hmgcr⁰¹¹⁵² double homozygous embryoshave severe patterning defects. However, in the remaining 60% the mesoderm appears correctly patterned but germ cell migration is severely defective (on average 14 germ cells remain on the gut), an enhancement over the germ cell migration phenotype seen in either single mutant. A similar degree of enhancement is seen in the presence of qm^L14.4/qm^L14.4. (Santos and Lehmann, 2004) In Hmgcr⁰¹¹⁵² homozygous embryos an average of 8 germ cells fail to migrate properly. (Santos and Lehmann, 2004) Weak germ cell migration defect. (Van Doren and Lehmann, 2001.2.14) Homozygous embryos derived from females with Hmgcr⁺ activity do not have cuticle defects. Embryos derived from homozygous female germ line clones have cuticle defects, which can be partially paternally rescued. (Russell et al., 1998) Homozygous embryos do not exhibit cuticle defects. Germline clones produce eggs with poor cuticle development. (Perrimon et al., 1996)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference third instar larval stage morphogenetic furrow \| posterior to (Treisman and Rubin, 1996)
Additional Information
Statement Reference In eye disc, expressed in a strong stripe posterior to the morphogenetic furrow; weaker later component. (Treisman and Rubin, 1996)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)01152 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11522 ry⁵⁰⁶ P{PZ}Hmgcr⁰¹¹⁵²/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Location 3R:19559215-19559216 confirmed by FlyBase alignment of dbGSS accession AQ026166 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	3-1152 (Treisman and Rubin, 1996) P{PZ}Hmgcr⁰¹¹⁵² (FlyBase, 2005, FlyBase, 1992-, ) P{PZ}l(3)01152⁰¹¹⁵² (Treisman and Rubin, 1996, FlyBase, 1992-) P{ry^+t7.2=PZ}l(3)01152⁰¹¹⁵² (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000485
References ( 19 )
Research paper	Yi et al., 2006, Science 313(5791): 1301--1303 The mevalonate pathway controls heart formation in Drosophila by isoprenylation of G gamma 1. [FBrf0194083] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Santos and Lehmann, 2004, Dev. Cell 6(2): 283--293 Isoprenoids control germ cell migration downstream of HMGCoA reductase. [FBrf0174539] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Keller et al., 1998, Dev. Biol. 202(2): 157--171 A role for nautilus in the differentiation of muscle precursors. [FBrf0105257] Russell et al., 1998, Genome 41(1): 7--13 Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila. [FBrf0102000] Van Doren et al., 1998, Nature 396(6710): 466--469 HMG-CoA reductase guides migrating primordial germ cells. [FBrf0105951] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142] Treisman and Rubin, 1996, Mech. Dev. 56(1-2): 17--24 Targets of glass regulation in the Drosophila eye disc. [FBrf0089138] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material	Yi, 2006, Science 313(5791): [FBrf0195927] Deshpande and Schedl, 2005, Dev. Cell 9(5): HMGCoA reductase potentiates hedgehog signaling in Drosophila melanogaster. [FBrf0202563]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Van Doren and Lehmann, 2001.2.14, Question about Hmgcr mutations. Question about Hmgcr mutations. [FBrf0135282] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}Hmgcr01152 Insertion

Dmel\P{PZ}Hmgcr⁰¹¹⁵² Insertion