Dmel\P{GawB}dimm⁹²⁹ Insertion

General Information
Symbol	Dmel\P{GawB}dimm⁹²⁹	Species	D. melanogaster
Name		FlyBase ID	FBti0004282
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GawB}	Expression data	GAL4 reporter/driver
Affected gene(s)	crc, dimm, Scer\GAL4	Viability / fertility
Causes allele(s)	crc⁹²⁹, dimm⁹²⁹, Scer\GAL4^dimm-929	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2L ( 39C2 )	Sequence location	2L:21,312,920..21,313,354
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:21,312,920..21,313,354 (Hewes et al., 2000)
Orientation
Cytological location (computed by FlyBase)	39C2 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location	The insertion site was mapped within a SstI-EcoRI fragment in the large intron. The orientation of the insertion was not reported. (Hewes et al., 2000)
Sequence Data
Flanking sequence
Inserted Element
Construct	P{GawB}
Location-dependent role	GAL4 driver/enhancer trap (Brand and Perrimon, 1993)
Size	11.279Kb
Associated alleles	Scer\GAL4^wB w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	crc (Hewes et al., 1997, Hewes et al., 2000) dimm (Hewes et al., 2003, Hewes et al., 2006) Scer\GAL4 (Osterwalder et al., 2004, Cerrato et al., 2006, Hewes et al., 1997, Hewes et al., 2003, Luan et al., 2006, Taghert et al., 2001, Joshi et al., 2004, Astle et al., 2003, Park et al., 2004, Marques et al., 2003, Hewes et al., 2000, Grima et al., 2004, Miguel-Aliaga et al., 2004, Schaefer and Taghert, 1995, Seroude et al., 2002, Hewes et al., 2006, Suster et al., 2003, Wegener et al., 2004, Jepson et al., 2012, Zhao et al., 2008, Kaplan et al., 2008, Johnson et al., 2005, Shafer and Taghert, 2009, Peabody et al., 2008, Grönke et al., 2009, Peabody et al., 2009, Donlea et al., 2009, Agrawal et al., 2010, Parisky et al., 2008, Nässel et al., 2008, Morton et al., 2008, Schroeder et al., 2003, Im and Taghert, 2010, Park et al., 2008, Jepson et al., 2011, Sundram et al., 2012, Saleem et al., 2012, Jansen et al., 2009, Kahsai and Winther, 2011)
Alleles and Phenotypes
Causes alleles	crc⁹²⁹ (Hewes et al., 1997, Hewes et al., 2003, Hewes et al., 2000, Schaefer and Taghert, 1995) dimm⁹²⁹ (Hewes et al., 2003, Hewes et al., 2006) Scer\GAL4^dimm-929 (Hewes et al., 1997, Osterwalder et al., 2004, Hewes et al., 2003, Luan et al., 2006, Taghert et al., 2001, Joshi et al., 2004, Astle et al., 2003, Park et al., 2004, Marques et al., 2003, Hewes et al., 2000, Grima et al., 2004, Miguel-Aliaga et al., 2004, Schaefer and Taghert, 1995, Seroude et al., 2002, Cerrato et al., 2006, Hewes et al., 2006, Suster et al., 2003, Wegener et al., 2004, Jepson et al., 2012, Zhao et al., 2008, Kaplan et al., 2008, Johnson et al., 2005, Shafer and Taghert, 2009, Peabody et al., 2008, Grönke et al., 2009, Peabody et al., 2009, Donlea et al., 2009, Agrawal et al., 2010, Parisky et al., 2008, Nässel et al., 2008, Morton et al., 2008, Schroeder et al., 2003, Im and Taghert, 2010, Park et al., 2008, Jepson et al., 2011, Sundram et al., 2012, Saleem et al., 2012, Jansen et al., 2009, Kahsai and Winther, 2011)

Lethality References partially lethal - majority die (Hewes et al., 2000) viable (Hewes et al., 2000)
Sterility References
Phenotype Manifest In
	adult head \| P-stage (Hewes et al., 2000) pupal abdomen (Hewes et al., 2000) ventral thoracic disc (Hewes et al., 2000)
Detailed Description
Statement Reference Hemizygous lethal phase is primarily after pupariation. Larvae with multiple mouthparts are not seen and puparia are normal in size and shape. Gas bubble translocation, expulsion of the larval tracheae and mouthparts and head eversion are all completed successfully. The distal portion of the everted leg discs often darken abnormally and do not differentiate further. After pupation, the abdomen shrinks markedly and withdraws to a dorsal position in these animals. Subsequently, the head collapses partially or completely into the thoracic cavity. Despite this "head/abdomen-collapsed" phenotype, many pupae develop eye pigmentation and other signs of adult differentiation. crc⁹²⁹ shows the "head/abdomen-collapsed" phenotype when heterozygous over some second chromosome balancers, suggesting haploinsufficiency for crc in some genetic backgrounds. (Hewes et al., 2000)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference larval stage ring gland (Hewes et al., 2000) embryonic/larval epidermis \| restricted (Hewes et al., 2000) embryonic/larval fat body (Hewes et al., 2000) neurosecretory neuron of embryonic/larval central nervous system (Hewes et al., 2000) embryonic/larval salivary gland (Hewes et al., 2000) distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference second instar larval stage -- third instar larval stage peritracheal cell (O'Brien and Taghert, 1998) third instar larval stage embryonic/larval corpus cardiacum (Nässel et al., 2008) neurosecretory cell of pars intercerebralis (Nässel et al., 2008) embryonic/larval central nervous system \| restricted (Hewes et al., 2006) embryonic/larval corpus cardiacum \| restricted (Hewes et al., 2006) Tv neuron (Hewes et al., 2006) Tvb neuron (Hewes et al., 2006) ventral nerve cord \| lateral & restricted (Hewes et al., 2006) pupal stage pupal Pdf neuron close to tritocerebrum (Shafer and Taghert, 2009) adult stage l-LNv neuron (Shafer and Taghert, 2009) small adult Pdf neuron of abdominal segment (Shafer and Taghert, 2009) adult brain \| restricted (Hewes et al., 2006) neurosecretory neuron of adult brain \| restricted (Hewes et al., 2006)
Additional Information
Statement Reference Scer\GAL4[dimm-929] is expressed in the pupal Pdf neuron close to tritocerebrum, which survive in newly eclosed adults but rapidly undergo apoptosis shortly thereafter. (Shafer and Taghert, 2009) Scer\GAL4[dimm-929] is expressed in a series of lateral abdominal neurons, and in some insulin-like producing cells (IPCs). (Hewes et al., 2006) In larva, expressed in peptidergic neurons of the CNS, intrinsic cells of the ring gland, salivary glands, fat body, patches of the epidermis, the PM peritracheal cells and a few other scattered locations; very restricted expression in the imaginal discs; no detectable expression in the skeletal muscles or the adbominal histoblasts. (Hewes et al., 2000) Expression is observed in peritracheal cells prior to second to third instar larval ecdysis. (O'Brien and Taghert, 1998)

Marker for	peritracheal cell (O'Brien and Taghert, 1998)
Reflects expression of
Reporter construct used in assay	Avic\GFP^Scer\UAS.cYa (Hewes et al., 2006) Dcr-2^Scer\UAS.cDa (Shafer and Taghert, 2009) dimm^{Scer\UAS.T:Hsap\MYC} (Hewes et al., 2006) Ecol\lacZ^{Scer\UAS.T:SV40\nls2} (Shafer and Taghert, 2009) Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} (Nässel et al., 2008) Pdf^GD299 (Shafer and Taghert, 2009)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	25373 w^*; P{GawB}dimm⁹²⁹ crc⁹²⁹
Linkouts
Comments
	The P{GawB}crc⁹²⁹ insertion maps within a SstI-EcoRI fragment in the large intron. The orientation of the insertion was not reported. (Hewes et al., 2000)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	C929 (Marques et al., 2003, Donlea et al., 2009) c929 (Sundram et al., 2012) c929(dimm) (Park et al., 2008) C929>Gal4 (Marques et al., 2003) c929G4 (Sundram et al., 2012) C929-GAL4 (Luan et al., 2006, Grima et al., 2004, Astle et al., 2003) c929-GAL4 (Miguel-Aliaga et al., 2004, Im and Taghert, 2010, Suster et al., 2003, Parisky et al., 2008, Jansen et al., 2009, Kahsai and Winther, 2011) c929-gal4 (Taghert et al., 2001, Schroeder et al., 2003, Park et al., 2008, Wegener et al., 2004) c929-Gal4 (Osterwalder et al., 2004, Zhao et al., 2008, Shafer and Taghert, 2009, Peabody et al., 2008, Peabody et al., 2009, Saleem et al., 2012, Nässel et al., 2008) C929-Gal4 (Kaplan et al., 2008) c929GAL4 (Agrawal et al., 2010) crc929 (Cerrato et al., 2006) dimm-Gal4 (Hewes et al., 2006, Jepson et al., 2011, Kahsai and Winther, 2011) dimm-GAL4 (Morton et al., 2008) P[c929^GAL4] (Park et al., 2004) P{GAL4}^c929 (Johnson et al., 2005) P{GAL4}ATF-4⁹²⁹ (Hewes et al., 1997, FlyBase, 1992-) P{GAL4}crc⁹²⁹ (FlyBase, 1992-) P{GawB}c929 (Seroude et al., 2002) P{GawB}crc⁹²⁹ (Hewes et al., 2006, FlyBase, 1992-, Hewes et al., 2000, Osterwalder et al., 2004, Zhao et al., 2008) P{GawB}dimm⁹²⁹ (FlyBase, 1992-, ) P{w^+mW.hs=GawB}dimm⁹²⁹ (Grönke et al., 2009)
Secondary FlyBase IDs

References ( 43 )

Generate a list of	All references relating to this insertion ( 43 )

List References by type	Research paper ( 40 ) Abstract ( 2 ) FlyBase analysis ( 1 )
Recent research papers ( 5 )
	Jepson et al., 2012, PLoS Genet. 8(4): e1002671 dyschronic, a Drosophila Homolog of a Deaf-Blindness Gene, Regulates Circadian Output and Slowpoke Channels. [FBrf0218124] Saleem et al., 2012, Mech. Dev. 129(5-8): 177--191 Drosophila melanogaster p24 trafficking proteins have vital roles in development and reproduction. [FBrf0219054] Sundram et al., 2012, J. Biol. Rhythms 27(3): 183--195 Cellular Requirements for LARK in the Drosophila Circadian System. [FBrf0218481] Jepson et al., 2011, J. Biol. Chem. 286(10): 8325--8337 Engineered Alterations in RNA Editing Modulate Complex Behavior in Drosophila: REGULATORY DIVERSITY OF ADENOSINE DEAMINASE ACTING ON RNA (ADAR) TARGETS. [FBrf0213236] Kahsai and Winther, 2011, J. Comp. Neurol. 519(2): 290--315 Chemical neuroanatomy of the Drosophila central complex: distribution of multiple neuropeptides in relation to neurotransmitters. [FBrf0212649] Show all research papers ...

Dmel\P{GawB}dimm929 Insertion

Dmel\P{GawB}dimm⁹²⁹ Insertion