Dmel\P{PZ}pum⁰²⁰⁰³ Insertion

General Information
Symbol	Dmel\P{PZ}pum⁰²⁰⁰³	Species	D. melanogaster
Name		FlyBase ID	FBti0004304
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data
Affected gene(s)	Ecol\lacZ, pum	Viability / fertility
Causes allele(s)	Ecol\lacZ^pum-02003, pum⁰²⁰⁰³	Stock availability	none publicly available
LINE ID	fs(2)02003 fs(3)02003
Genomic Location
Chromosomal location	3R ( 85C11 )	Sequence location	3R:4,983,704..4,983,704 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:4,983,704..4,983,704 [+] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	85C11 ( inferred by FlyBase from sequence location )
Cytological location (reported)	85D-85D (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025572 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Lin and Spradling, 1997) pum (Lin and Spradling, 1997)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^pum-02003 (Lin and Spradling, 1997) pum⁰²⁰⁰³ (Lin and Spradling, 1997, Spradling, 1999.12.9, Parisi and Lin, 1999)

Lethality References
Sterility References female sterile (Spradling et al., 1997, Lin and Spradling, 1997) sterile (Ye et al., 2004)
Phenotype Manifest In
	female germline stem cell (Spradling et al., 1997, Lin and Spradling, 1997) ovariole \| germline clone (Parisi and Lin, 1999) ovary (Parisi and Lin, 1999, Lin and Spradling, 1997)
Detailed Description
Statement Reference pum⁰²⁰⁰³ bam^Δ86/pum⁹ bam^Δ86 double mutant ovaries have a complex phenotype that is distinct from that of either single mutant. A mixture of apparently undifferentiated cells and overtly polyploid cells is seen. In many cases, the polyploid chromosomes are thick and expanded like nurse cell chromosomes. Occasionally these pseudo-nurse cells are organised within an epithelial layer of follicle cells, like a cyst, although these cysts never contain a full complement of 16 cystocytes. These cysts contain ring canals. (Chen and McKearin, 2005) Mutants exhibit increased long term facilitation (LTF) at the neuromuscular junction. (Schweers et al., 2002) Homozygous females do not lay eggs. Approximately 50% of homozygous larvae survive the pupal stage to eclosion. By the adult stage, 43% of homozygous females have germlineless ovaries, 32% have "differentiated ovaries" (containing recognisable egg chambers and/or germline cells in at least one ovariole) and 25% of females have one germlineless and one differentiated ovary. Homozygous female germline clones induced at the third larval instar stage produce ovarioles that contain on average 4.2 +/- 2.0 mature egg chambers but no other germ cells. Females containing homozygous female germline clones achieve an oviposition efficiency of 56% (for absolute number of eggs laid by the females). These eggs (derived from females containing homozygous germline clones mated to homozygous males) are sometimes capable of developing into adulthood. (Parisi and Lin, 1999) pum⁰¹⁶⁸⁸ partially complements pum⁰²⁰⁰³ for oogenic defects and embryonic lethality. (Parisi and Lin, 1999) Small ovary phenotype. None of the germ cells appear to undergo asymmetric divisions. Ovarioles contain only two clusters of apparently undifferentiated germline cells. Some ovaries in newly eclosed females completely lack germline cells though individual ovarioles are visible, in other ovaries the ovarioles are not recognisable. A small number of ovarioles contain 2-3 normally developing egg chambers that eventually develop into morphologically normal mature oocytes. Preoogenic germline development is also aberrant, germline stem cells of late third instar larval ovaries have no obvious spectrosome organisation. (Lin and Spradling, 1997) Females are not able to maintain a normal germarial structure: instead of retaining active stem cells each ovariole houses only one or two germ line derivatives. When the non-functional egg chambers leave the germarium they are not replaced, the germarium assumes the appearance of a thin tube. Stem cells cannot be maintained and instead differentiate into cystoblast-like cells. (Spradling et al., 1997)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	fs(2)02003 (Gene Disruption Project members, 2001-) fs(3)02003 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line	t (Gene Disruption Project members, 2001-)
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 3R:4983703-4983704 determined by FlyBase alignment of dbGSS accession AQ025572 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005) This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	fs(2)02003 (FlyBase, 1992-) P{PZ}02003 (FlyBase, 1992-) P{PZ}pum⁰²⁰⁰³ (FlyBase, 2005, FlyBase, 1992-, ) P{PZ}pum²⁰⁰³ (Lin and Spradling, 1997, FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0010313
References ( 15 )
Research paper	Chen and McKearin, 2005, Curr. Biol. 15(2): 179--184 Gene circuitry controlling a stem cell niche. [FBrf0183822] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Ye et al., 2004, Curr. Biol. 14(4): 314--321 Nanos and Pumilio are essential for dendrite morphogenesis in Drosophila peripheral neurons. [FBrf0174478] Schweers et al., 2002, Genetics 161(3): 1177--1185 The Drosophila melanogaster translational repressor pumilio regulates neuronal excitability. [FBrf0151333] Parisi and Lin, 1999, Genetics 153(1): 235--250 The Drosophila pumilio gene encodes two functional protein isoforms that play multiple roles in germline development, gonadogenesis, oogenesis and embryogenesis. [FBrf0111454] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Lin and Spradling, 1997, Development 124(12): 2463--2476 A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary. [FBrf0095585] Spradling et al., 1997, Cold Spring Harbor Symp. Quant. Biol. 62: 25--34 The Drosophila germarium: stem cells, germ line cysts, and oocytes. [FBrf0102402] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Spradling, 1999.12.9, Helping FlyBase with P-element paper. Helping FlyBase with P-element paper. [FBrf0125024] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}pum02003 Insertion

Dmel\P{PZ}pum⁰²⁰⁰³ Insertion