Dmel\P{PZ}Hsc70-4⁰³⁵⁵⁰ Insertion

General Information
Symbol	Dmel\P{PZ}Hsc70-4⁰³⁵⁵⁰	Species	D. melanogaster
Name		FlyBase ID	FBti0004312
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Hsc70-4	Viability / fertility
Causes allele(s)	Ecol\lacZ^{Hsc70-4-03550}, Hsc70-4⁰³⁵⁵⁰	Stock availability	1 publicly available
LINE ID	l(3)03550
Genomic Location
Chromosomal location	3R ( 88E4 )	Sequence location	3R:11,068,403..11,068,403 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:11,068,403..11,068,403 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	88E4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	88E9 (inferred from flanking sequence analysis) (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034056 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Schmucker et al., 1997, Braun et al., 1997, Braun et al., 1998, Vosshall, 1998.8.10) Hsc70-4 (BDGP Project Members, 1994-1999, Schmucker et al., 1997)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^{Hsc70-4-03550} (Schmucker et al., 1997, Braun et al., 1997, Braun et al., 1998, Vosshall, 1998.8.10) Hsc70-4⁰³⁵⁵⁰ (Schmucker et al., 1997, BDGP Project Members, 1994-1999, Brumby et al., 2002, Hing et al., 1999, Doughty, 2000.5.9, Braun et al., 1997, Spradling et al., 1999)

Lethality References lethal (Mollaaghababa et al., 2001) lethal \| embryonic stage \| maternal effect \| poor (Perrimon et al., 1996) lethal \| pupal stage \| recessive (Braun et al., 1997) lethal \| recessive (Spradling et al., 1999, Perrimon et al., 1996) partially lethal - majority die (Bronk et al., 2001, )
Sterility References
Phenotype Manifest In
	abdominal segment 1 \| maternal effect (Perrimon et al., 1996) abdominal segment 2 \| maternal effect (Perrimon et al., 1996) abdominal segment 3 \| maternal effect (Perrimon et al., 1996) abdominal segment 4 \| maternal effect (Perrimon et al., 1996) abdominal segment 5 \| maternal effect (Perrimon et al., 1996) abdominal segment 6 \| maternal effect (Perrimon et al., 1996) abdominal segment 7 \| maternal effect (Perrimon et al., 1996) abdominal segment 8 \| maternal effect (Perrimon et al., 1996) abdominal segment 9 \| maternal effect (Perrimon et al., 1996) abdominal segment 10 \| maternal effect (Perrimon et al., 1996) abdominal segment 11 \| maternal effect (Perrimon et al., 1996) Bolwig nerve (Schmucker et al., 1997) Bolwig organ (Schmucker et al., 1997) embryonic/first instar larval cuticle \| maternal effect (Perrimon et al., 1996) head \| anterior \| maternal effect (Perrimon et al., 1996) melanotic mass (Braun et al., 1997) photoreceptor cell (Schmucker et al., 1997)
Detailed Description
Statement Reference The CycE^JP rough eye phenotype is weakly suppressed by the addition of Hsc70-4⁰³⁵⁵⁰. (Brumby et al., 2002) The EJP amplitudes recorded from Hsc70-4⁰³⁵⁵⁰, Csp^U1 double mutant neuromuscular junctions (NMJs) are reduced to about a third of control amplitudes - about the same as each allele individually. The frequency and amplitude of spontaneously occurring mEJPs in double mutants are not different from controls. (Bronk et al., 2001) The nerve-evoked excitatory junctional potential (EJP) amplitudes in mutant larvae are reduced to about half that of controls. EJP amplitudes are not reduced by a fatigue of evoked release, because responses sequentially recorded at 0.2 Hz do not progressively diminish. The quantal content of evoked release in homozygotes is reduced to a third to a half of control. (Bronk et al., 2001) Shows no interaction with N pathway mutants. (Hing et al., 1999) Third instar larvae contain circulating melanotic capsules, and die as early pupae. (Braun et al., 1997) The Bolwig\'s organs show variable disruption of cell clustering in homozygous embryos. A number of photoreceptors are displaced along the path of the Bolwig\'s nerve (BN) in some embryos. The BN defasciculates on the surface of the optic lobe anlagen in most embryos. the defasciculated BN usually projects abnormally and forms loop-like structures. (Schmucker et al., 1997) Germline clones produce eggs with patterning defects: anterior head defects and variable abdominal segmentation defects. Lethality occurs during the larval and pupal stages. (Perrimon et al., 1996) visible rough eyes, bent wings, thin, short bristles
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) embryonic/larval midgut \| restricted (Meister and Braun, 1995.10) gonad (Meister and Braun, 1995.10) Malpighian tubule (Meister and Braun, 1995.10) adult stage adult brain (Vosshall, 1998.8.10)
Additional Information
Statement Reference In adult, expression throughout brain. (Vosshall, 1998.8.10)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)03550 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	5807 ru¹ h¹ th¹ st¹ Ki¹ p^p P{PZ}Hsc70-4⁰³⁵⁵⁰/TM3, Sb¹
Linkouts
Comments
	Location 3R:11068403-11068404 confirmed by FlyBase alignment of dbGSS accession AQ034056 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1594 (Meister and Braun, 1995.10) P{PZ}E(nd)195^ry+3550 (Doughty, 2000.5.9) P{PZ}Hsc70-4⁰³⁵⁵⁰ (FlyBase, 2005, FlyBase, 1992-, ) P{PZ}l(3)03550⁰³⁵⁵⁰ (FlyBase, 1992-) P{PZ}scattered⁰³⁵⁵⁰ (Schmucker et al., 1997, FlyBase, 1992-) P{ry^+t7.2=PZ}l(3)03550⁰³⁵⁵⁰ (FlyBase, 1992-) PZ[ry+3550] (Hing et al., 1999)
Secondary FlyBase IDs
	FBti0000534
References ( 19 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Brumby et al., 2002, EMBO J. 21(13): 3377--3389 Drosophila cyclin E interacts with components of the Brahma complex. [FBrf0151282] Bronk et al., 2001, Neuron 30(2): 475--488 Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo. [FBrf0137066] Mollaaghababa et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(7): 3958--3963 Mutations in Drosophila heat shock cognate 4 are enhancers of Polycomb. [FBrf0135926] Hing et al., 1999, Europ. J. Cell Biol. 78(10): 690--697 Mutations in the heatshock cognate 70 protein (hsc4) modulate Notch signaling. [FBrf0123050] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Braun et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95(24): 14337--14342 Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes. [FBrf0105781] Braun et al., 1997, Genetics 147(2): 623--634 Drosophila immunity: analysis of larval hemocytes by P-element-mediated enhancer trap. [FBrf0098749] Schmucker et al., 1997, Development 124(5): 937--948 Genetic analysis of the larval optic nerve projection in Drosophila. [FBrf0092677] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Doughty, 2000.5.9, P{PZ}E(nd)195<up>ry+3550</up>. P{PZ}E(nd)195^ry+3550. [FBrf0128184] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}Hsc70-403550 Insertion

Dmel\P{PZ}Hsc70-4⁰³⁵⁵⁰ Insertion