Dmel\P{GAL4}A307 Insertion

General Information
Symbol	Dmel\P{GAL4}A307	Species	D. melanogaster
Name		FlyBase ID	FBti0004408
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GAL4}	Expression data	GAL4 reporter/driver
Affected gene(s)	Scer\GAL4	Viability / fertility
Causes allele(s)	Scer\GAL4^A307	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	2	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	2 (Allen et al., 1997)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{GAL4}
Location-dependent role	GAL4 driver/enhancer trap (FlyBase, 1992-)
Size
Associated alleles	Scer\GAL4^unspecified
Molecular map
Affected Gene(s)
Insertion may affect gene	Scer\GAL4 (Phelan et al., 1996, Smith et al., 1996, Murphey et al., 2003, Godenschwege et al., 2002, White et al., 2001, Presente et al., 2002, Allen et al., 1999, Blagburn et al., 1999, Allen et al., 1997, Allen et al., 1998, Allen et al., 1997, Allen et al., 1995, Godenschwege et al., 2006, Storkebaum et al., 2009, Zhang et al., 2009, Allen et al., 2000, Ma et al., 2009, Zhang et al., 2007)
Alleles and Phenotypes
Causes alleles	Scer\GAL4^A307 (Phelan et al., 1996, Smith et al., 1996, Murphey et al., 2003, Godenschwege et al., 2002, White et al., 2001, Presente et al., 2002, Allen et al., 1999, Blagburn et al., 1999, Allen et al., 1997, Allen et al., 1998, Allen et al., 1997, Allen et al., 1995, Godenschwege et al., 2006, Storkebaum et al., 2009, Zhang et al., 2009, Allen et al., 2000, Ma et al., 2009, Zhang et al., 2007)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference third instar larval stage -- adult stage giant fiber neuron (Allen et al., 1998) prepupal stage giant fiber neuron (Smith et al., 1996) prepupal stage P3 -- pharate adult stage P15 giant fiber neuron (Allen et al., 1997) Comment:>=6 hrs APF adult stage giant fiber neuron (Allen et al., 1997, Blagburn et al., 1999) tergotrochanteral muscle motor neuron (Allen et al., 1998) peripherally synapsing interneuron (Allen et al., 1998)
Additional Information
Statement Reference The cell body of the giant fibre neuron is located just below the dorsal protocerebral surface. The major dendritic arborization projects posterolaterally and ventrally, arborizing in the deutocerebrum. Minor dendrites project ventrolaterally and dorsomedially. The latter follow the path of the giant commissure interneurons (which also mark with Scer\GAL4[A307]). The axon runs posteriorly towards the ventral midline, and projects dorsally, exiting the brain along the dorsal midline of the cervical connective. It projects unbranched through the prothoracic neuromere until it reaches the mesothoracic neuromere where it turns ventrally and laterally towards the edge of the ganglion. (Allen et al., 1998) During pupal stage, expression first detected at 6hrs. APF; consistent expression in giant fibers; dynamic and transient expression in other cells of the CNS.In adults, predominant expression in the cell bodies and axons of the giant fibers; also in a small number of other cells of the CNS: in giant commissural interneurons, possibly targets of the GF's, and in a bilateral pattern of scattered cells in the brain and thoracic/abdominal ganglion. (Allen et al., 1997)

Marker for	adult mushroom body larval mushroom body antennal lobe \| precursor (Smith et al., 1996) giant fiber neuron (Allen et al., 1999) giant fibers (Allen et al., 1999)
Reflects expression of
Reporter construct used in assay	Ecol\lacZ^Scer\UAS.cBa (Allen et al., 1998) n-syb^{Scer\UAS.cEa.T:Avic\GFP} (Blagburn et al., 1999)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	A307 (Godenschwege et al., 2002, Blagburn et al., 1999, Godenschwege et al., 2006, Allen et al., 2000, Ma et al., 2009) A307-Gal4 (Presente et al., 2002, White et al., 2001, Zhang et al., 2007, Zhang et al., 2009) A307-GAL4 (Storkebaum et al., 2009) P[Gal4]-A307 (Murphey et al., 2003) P[GAL4]A307 (Allen et al., 1998) P{GAL4}307 (Smith et al., 1996, FlyBase, 1992-) P{GAL4}A307 (Phelan et al., 1996, FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0004212
References ( 19 )
Research paper	Ma et al., 2009, BMC Genet. 10: 77 A modifier screen in the Drosophila eye reveals that aPKC interacts with Glued during central synapse formation. [FBrf0209456] Storkebaum et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(28): 11782--11787 Dominant mutations in the tyrosyl-tRNA synthetase gene recapitulate in Drosophila features of human Charcot–Marie–Tooth neuropathy. [FBrf0208295] Zhang et al., 2009, Dis. Model Mech. 2(5-6): 247--266 Inactivation of Drosophila Huntingtin affects long-term adult functioning and the pathogenesis of a Huntington's disease model. [FBrf0208014] Zhang et al., 2007, Europ. J. Neurosci. 26(9): 2405--2416 A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons. [FBrf0201582] Godenschwege et al., 2006, Curr. Biol. 16(1): 12--23 A conserved role for Drosophila Neuroglian and human L1-CAM in central-synapse formation. [FBrf0190016] Murphey et al., 2003, Development 130(16): 3671--3682 Targeted expression of shibire[ts] and semaphorin 1a reveals critical periods for synapse formation in the giant fiber of Drosophila. [FBrf0160808] Godenschwege et al., 2002, J. Neurosci. 22(8): 3117--3129 Ectopic expression in the giant fiber system of Drosophila reveals distinct roles for roundabout (Robo), Robo2, and Robo3 in dendritic guidance and synaptic connectivity. [FBrf0147116] Presente et al., 2002, genesis 34(1-2): 165--169 Transgene-mediated RNA interference defines a novel role for Notch in chemosensory startle behavior. [FBrf0152371] White et al., 2001, Neuron 31(5): 699--711 Targeted attenuation of electrical activity in Drosophila using a genetically modified K[+] channel. [FBrf0139774] Allen et al., 2000, Mol. Cell. Neurosci. 16(6): 754--765 A role for Drosophila Drac1 in neurite outgrowth and synaptogenesis in the Giant Fiber System. [FBrf0132411] Allen et al., 1999, J. Neurosci. 19(21): 9374--9384 Targeted expression of truncated glued disrupts giant fiber synapse formation in Drosophila. [FBrf0111772] Blagburn et al., 1999, J. Comp. Neurol. 404(4): 449--458 Null mutation in shaking-B eliminates electrical, but not chemical, synapses in the Drosophila giant fiber system: a structural study. [FBrf0106230] Allen et al., 1998, J. Comp. Neurol. 397(4): 519--531 Development of the giant fiber neuron of Drosophila melanogaster. [FBrf0103226] Phelan et al., 1996, J. Neurosci. 16(3): 1101--1113 Mutations in shaking-B prevent electrical synapse formation in the Drosophila giant fiber system. [FBrf0085744] Smith et al., 1996, Dev. Genes Evol. 206(1): 14--24 Inducible ternary control of transgene expression and cell ablation in Drosophila. [FBrf0090807]
Abstract	Allen et al., 1997, Doe, Hall, 1997: 18 Analysis of a P[GAL4] enhancer-trap expressed in the giant fibre system. [FBrf0099086] Allen et al., 1997, J. Neurogenet. 11: 142 Development of the giant fiber system (GFS). [FBrf0104972] Allen et al., 1995, Posakony, White, 1995: 20 Development of the giant fiber neuron. [FBrf0083735]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]