Dmel\P{PZ}Itp-r83A⁰⁵⁶¹⁶ Insertion

General Information
Symbol	Dmel\P{PZ}Itp-r83A⁰⁵⁶¹⁶	Species	D. melanogaster
Name		FlyBase ID	FBti0004437
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Itp-r83A, Nmdar1	Viability / fertility
Causes allele(s)	Ecol\lacZ^{Itp-r83A-05616}, Itp-r83A⁰⁵⁶¹⁶, Nmdar1⁰⁵⁶¹⁶	Stock availability	1 publicly available
LINE ID	l(3)05616
Genomic Location
Chromosomal location	3R ( 83A7 )	Sequence location	3R:1,343,232..1,343,232 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:1,343,232..1,343,232 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	83A7 ( inferred by FlyBase from sequence location )
Cytological location (reported)	83A5-83A6 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034066 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Venkatesh and Hasan, 1997, Vosshall, 1998.8.10) Itp-r83A (Venkatesh and Hasan, 1997) Nmdar1 (FlyBase, 1996-)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^{Itp-r83A-05616} (Venkatesh and Hasan, 1997, Vosshall, 1998.8.10) Itp-r83A⁰⁵⁶¹⁶ (Venkatesh and Hasan, 1997, Spradling et al., 1999, BDGP Project Members, 1994-1999, Joshi et al., 2004, Sinha and Hasan, 1999, Venkatesh et al., 2001, FlyBase, 1996-) Nmdar1⁰⁵⁶¹⁶ (FlyBase, 1996-)

Lethality References lethal \| pupal stage (Venkatesh et al., 2001) lethal \| pupal stage \| recessive (Venkatesh et al., 2001, Venkatesh and Hasan, 1997) lethal \| recessive (Spradling et al., 1999) viable (Raghu et al., 2000)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference rut²⁰⁸⁰, Itp-r83A⁰⁵⁶¹⁶ double mutants fail to moult from the second to the third larval instar and perish in the second larval instar. Several of the double mutant larvae have tracheal endings characteristic of the first instar larva. (Venkatesh et al., 2001) Homozygotes can molt to the third instar and eventually die primarily as pupae. A few adult survivors do survive. (Venkatesh et al., 2001) The response of homozygous Itp-r83A⁰⁵⁶¹⁶ and Itp-r83A⁰⁵⁶¹⁶/Itp-r83A^XR12 survivors to all concentrations of ethyl acetate is normal. (Deshpande et al., 2000) Almost 50% of homozygotes moult into third instar larvae and pupae. Both moults are delayed compared to heterozygotes. The remaining 50% of homozygotes survive at the second larval stage for an extended time period and then appear to moult directly into pupae. Very few homozygous adults eclose, those that do have no obvious morphological defects. The larval moulting delay in homozygotes is rescued by 20-hydroxyecdysone. (Venkatesh and Hasan, 1997)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference third instar larval stage myoblast of imaginal disc (Venkatesh and Hasan, 1997) ring gland (Venkatesh and Hasan, 1997) embryonic/larval salivary gland (Venkatesh and Hasan, 1997) wandering third instar larval stage embryonic/larval fat body (Meister and Braun, 1995.10)
Additional Information
Statement Reference Expression is detected in the ring gland and in the salivary gland in addition to the previously observed expression in myoblasts within imaginal discs. (Venkatesh and Hasan, 1997) In wandering third instar larva, weak expression in fat body. (Meister and Braun, 1995.10)

Marker for
Reflects expression of	Itp-r83A (Venkatesh and Hasan, 1997)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)05616 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11664 P{PZ}Itp-r83A⁰⁵⁶¹⁶ Nmdar1⁰⁵⁶¹⁶ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Location 3R:1343232-1343233 confirmed by FlyBase alignment of dbGSS accession AQ034066 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1664 (Meister and Braun, 1995.10) P{PZ}Itp-r83A⁰⁵⁶¹⁶ (FlyBase, 2005, Venkatesh and Hasan, 1997, FlyBase, 1992-) P{PZ}l(3)05616⁰⁵⁶¹⁶ (FlyBase, 1992-) P{ry^+t7.2=PZ}l(3)05616⁰⁵⁶¹⁶ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000591
References ( 16 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Joshi et al., 2004, Genetics 166(1): 225--236 Genetic dissection of itpr gene function reveals a vital requirement in aminergic cells of Drosophila larvae. [FBrf0174703] Venkatesh et al., 2001, Genetics 158(1): 309--318 Interactions between the inositol 1,4,5-trisphosphate and cyclic amp signaling pathways regulate larval molting in Drosophila. [FBrf0135810] Deshpande et al., 2000, J. Neurobiol. 43(3): 282--288 The inositol 1,4,5-trisphosphate receptor is required for maintenance of olfactory adaptation in Drosophila antennae. [FBrf0128448] Raghu et al., 2000, Molec. Cell. Neurosci. 15(5): 429--445 Normal phototransduction in Drosophila photoreceptors lacking an InsP[3] receptor gene. [FBrf0128616] Sinha and Hasan, 1999, Gene 233(1-2): 271--276 Sequencing and exon mapping of the inositol 1,4,5-trisphosphate receptor cDNA from Drosophila embryos suggests the presence of differentially regulated forms of RNA and protein. [FBrf0110814] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Venkatesh and Hasan, 1997, Curr. Biol. 7(7): 500--509 Disruption of the IP₃ receptor gene of Drosophila affects larval metamorphosis and ecdysone release. [FBrf0097686] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}Itp-r83A05616 Insertion

Dmel\P{PZ}Itp-r83A⁰⁵⁶¹⁶ Insertion