Dmel\Stalker2{}ovo^D1rv23 Insertion

General Information
Symbol	Dmel\Stalker2{}ovo^D1rv23	Species	D. melanogaster
Name		FlyBase ID	FBti0004660
Feature type	transposable_element_insertion_site
Description
Inserted element	HMS-Beagle	Expression data
Affected gene(s)	ovo	Viability / fertility
Causes allele(s)	ovo^D1rv23	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	X ( 4E2 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	4E2 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	HMS-Beagle
Location-dependent role
Size
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene	ovo (Garfinkel et al., 1992)
Alleles and Phenotypes
Causes alleles	ovo^D1rv23 (Salles et al., 2002, Garfinkel et al., 1992)

Lethality References
Sterility References female sterile \| recessive (Andrews et al., 2000, Oliver et al., 1990, Mevel-Ninio et al., 1991, Oliver and Pauli, 1998) male fertile (Oliver et al., 1987)
Phenotype Manifest In
	egg chamber (Mevel-Ninio et al., 1991, Oliver et al., 1987, Hinson et al., 1999) germarium cap cell (Song et al., 2007) germline cell (Nagoshi et al., 1995) germline cell \| embryonic stage (Oliver et al., 1990) germline cell \| female (Andrews et al., 2000, Hinson et al., 1999) nurse cell (Hinson et al., 1999) nurse cell fusome (Hinson et al., 1999) ovary (Waterbury et al., 2000, Granadino et al., 1992, Oliver and Pauli, 1998, Oliver et al., 1994, Salles et al., 2002)
Detailed Description
Statement Reference Mutant germaria are agametic. In 2 day old mutant females, many germaria still contain one to five cap cells, although some germaria completely lack cap cells. (Song et al., 2007) Homozygous ovaries have an agametic phenotype. (Salles et al., 2002) ovo^D1rv23 females carrying two copies of ovo^-2b have ovaries with a variety of defects. Egg chambers in which follicle cells have failed to migrate and degenerating ovarioles are seen. Egg chambers develop to maturity in rare cases, giving rise to short, abnormal eggs which have ventralised eggshells and fused dorsal appendages. (Salles et al., 2002) Ovaries of homozygous females are devoid of germ cells. (Andrews et al., 2000) ovo^D1rv23 have no adverse effect on the development of the germline in XY tra^F.Hsp83 pseudofemales; the pseudo-ovaries are filled with a multitude of undifferentiated germ cells. (Waterbury et al., 2000) The ovaries of ovo^D1rv23 females are primarily agametic. (Waterbury et al., 2000) Addition of otu^hs.PN modestly improves the ovo^D1rv23 phenotype. In mutant females homozygous for ovo^D1rv23 and with one copy of otu^hs.PN, the number of ovary lobes seen with at least one egg chamber increases. However a small number of egg chambers per ovary are seen, with almost all of these displaying a tumorous phenotype, and those without this phenotype have a mixture of tumorous and nurse-like cells. In addition there is some improvement in the degree of oogenic differentiation as the frequency of germ cell clusters with hts positive ring canals or nurse-like cells increases. However chambers remain small and do not progress beyond stage 4. The addition of more than one copy of otu^hs.PN have no extra effect. The addition of otu^hs.PN to ovo^D1rv23/ovo^M1, ovo^D1rv23/ovo^M2 or ovo^D1rv23/ovo^e8F mothers leads to an increase in the number of eggs produced, though in no case is hatching observed. (Hinson et al., 1999) XX tra¹/tra⁴ pseudomales that are also homozygous for ovo^D1rv23 show an increase in the frequency of atrophic gonads compared to XX tra¹/tra⁴ pseudomales. Non-oogenic gonads, with spectrosomes and multibranched fusomes are also seen (at the same frequency as XX tra¹/tra⁴ pseudomales). Oogenic gonads are not seen in XX tra¹/tra⁴ pseudomales carrying otu^hs.PN that are also homozygous for ovo^D1rv23. In each case the frequency of non-oogenic gonads is increased compared to XX tra¹/tra⁴ pseudomales also homozygous for ovo^D1rv23. (Hinson and Nagoshi, 1999) In homozygous mutant germaria, very few ovary lobes contain any egg chambers. Few germ cells are found, those germ cells that are found usually have no spectrosome or fusome material. In those rare cases that do, fusomes are small and poorly branched indicating aberrant and aborted differentiation early in gametogenesis. Those rare egg chambers that are found are also small and have no nurse cells. ovo^e7E/ovo^D1rv23 mutants have egg chambers arrested in pre-vitellogenic stages. (Hinson et al., 1999) The ovary phenotype of homozygous ovo^D1rv23 females is partially suppressed if they are also carrying mle¹/mle⁵ or mle¹/mle¹⁴. The ovary phenotype is of ovo^D1rv23/ovo^D1rv22 females partially suppressed if they are also carrying mle¹/mle¹. The phenotype is not affected if the females are also carrying Sxl^M4 or Sxl^M1. The phenotype is not affected if the females are also carrying msl-2¹³⁸/msl-2¹³⁸ or msl-2¹³⁸/msl-2¹. ovo^D1rv23/ovo^D1rv22; mle⁹/mle¹⁴ ovaries are phenotypically mosaic, containing germ cells of both male and female morphology. The mosaicism can be at the level of whole ovaries or ovarioles. ovo^D1rv23/ovo^D1rv23; mle¹/mle⁵ ovaries most often contain germ cells of male morphology rather than female morphology. This phenotype is partially suppressed by Sxl^M1; ovaries more often contain germ cells of female morphology rather than male morphology. (Oliver and Pauli, 1998) Ovaries of homozygous females contain germline chambers in less than 20% of cases. Ovaries of ovo^D1rv23/ovo^D1rv22 females contain germline chambers in less than 20% of cases. (Oliver and Pauli, 1998) Homozygous female embryos contain the normal number of germ cells. Most homozygous female first instar larvae have the wild-type number of germ cells, although by the end of the third larval instar many homozygous female larvae have a reduced number or no germ cells. (Staab and Steinmann-Zwicky, 1996) XX females have agametic ovaries. ovo^D1rv23/Y pseudofemales (carrying tra^Hsp83.PS) have ovaries that contain large numbers of tumorous egg cysts. XX homozygotes transformed into pseudomales with tra¹/tra⁴ show pseudotestes characteristic of group 1, either empty or containing only undifferentiated germ cells. (Nagoshi et al., 1995) In contrast to a previous study (FBrf0046291) no significant reduction in the number of germ cells in female homozygous embryos was detected. Any early reduction in germ cell numbers is either transient or inconsistent. Many larval homozygous ovaries have near wild type morphology. Many larval ovaries of the agametic heteroallelic combination ovo^D1rv23/ovo^D1rv22 also have near wild type morphology. By late pupae homozygous gonads fail to produce egg chambers and germ cells that are present fail to differentiate. (Rodesch et al., 1995) Increased amounts of cuticular hydrocarbons. (Wicker and Jallon, 1995) Ovaries are atrophic and have no germ cells. Rare, escaping germ cells are usually found as single cells or small clusters in a single ovariole, though they sometimes form cysts. ovo^-,tra^hs.PB XY ovaries have abundant germ cells. The germ cells of XO ovo^- males have the crystals characteristic of XO males and are fully populated with male germ cells of all stages. (Oliver et al., 1994) The survival of 2X ovo^D1rv23/ovo^D1rv22 germline cells is enhanced in an mle¹/mle⁵ background. Surviving cells usually form small ovarian tumours. Sxl^M1 partially rescues the morphology of the otu mutant ovarian tumour cells. (Oliver et al., 1993) Phenotypic category: svb⁺ ovo^-. (Garfinkel et al., 1992) The germ cells of homozygous females die. Homozygous females also heterozygous for fl(2)d¹ (at 29^oC) or fl(2)d² (at 25^oC) have small ovaries with a few ovarioles containing mainly multicellular cysts and cysts with 16 cells resembling young egg chambers. (Granadino et al., 1992) Lack of egg chamber formation. (Mevel-Ninio et al., 1991) Germ cell death occurs during early gastrulation in female homozygotes. This phenotype is suppressed in females also homozygous for Sxl^f1. The soma is wild-type. (Oliver et al., 1990) Gene activity is not detectable. Viable. Male germ line is fertile, female germ line has no vitellogenic egg chambers. (Oliver et al., 1987)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	HMS-Beagle{}ovo^D1rv23 (FlyBase, 1992-) rom{}ovo^D1rv23 (FlyBase, 1992-) Stalker2{}ovo^D1rv23 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 19 )
Research paper	Song et al., 2007, Development 134(6): 1071--1080 Notch signaling controls germline stem cell niche formation in the Drosophila ovary. [FBrf0201538] Salles et al., 2002, Dev. Biol. 246(2): 366--376 A germline-specific splicing generates an extended ovo protein isoform required for Drosophila oogenesis. [FBrf0148939] Andrews et al., 2000, Development 127(4): 881--892 OVO transcription factors function antagonistically in the Drosophila female germline. [FBrf0125467] Waterbury et al., 2000, Genetics 155(4): 1741--1756 Sex determination in the Drosophila germline is dictated by the sexual identity of the surrounding soma. [FBrf0130161] Hinson et al., 1999, Mech. Dev. 88(1): 3--14 Regulatory and functional interactions between ovarian tumor and ovo during Drosophila oogenesis. [FBrf0111908] Hinson and Nagoshi, 1999, Development 126(5): 861--871 Regulatory and functional interactions between the somatic sex regulatory gene transformer and the germline genes ovo and ovarian tumor. [FBrf0106653] Oliver and Pauli, 1998, Dev. Genet. 23(4): 335--346 Suppression of distinct ovo phenotypes in the Drosophila female germline by maleless- and Sex-lethal. [FBrf0105902] Staab and Steinmann-Zwicky, 1996, Mech. Dev. 54(2): 205--210 Female germ cells of Drosophila require zygotic ovo and otu product for survival in larvae and pupae respectively. [FBrf0086636] Nagoshi et al., 1995, Development 121(2): 579--587 The somatic sex determines the requirement for ovarian tumor gene activity in the proliferation of the Drosophila germline. [FBrf0076142] Rodesch et al., 1995, Genetics 141(1): 191--202 Developmental analysis of the ovarian tumor gene during Drosophila oogenesis. [FBrf0084317] Wicker and Jallon, 1995, Europ. J. Ent. 92(1): 197--202 Influence of ovary and ecdysteroids on pheromone biosynthesis in Drosophila melanogaster, Diptera: Drosophilidae. [FBrf0080479] Oliver et al., 1994, Development 120(11): 3185--3195 Function of Drosophila ovo⁺ in germ-line sex determination depends on X-chromosome number. [FBrf0074069] Oliver et al., 1993, Development 119(3): 897--908 Sex-lethal, master and slave: a hierarchy of germ-line sex determination in Drosophila. [FBrf0065519] Garfinkel et al., 1992, Genetics 130(4): 791--803 Molecular genetics of the Drosophila melanogaster ovo locus, a gene required for sex determination of germline cells. [FBrf0056260] Granadino et al., 1992, Genetics 130: 597--612 Evidence of a dual function in fl(2)d, a gene needed for Sex-lethal expression in Drosophila melanogaster. [FBrf0056240] Mevel-Ninio et al., 1991, EMBO J. 10: 2259--2266 The ovo gene of Drosophila encodes a zinc finger protein required for female germ line development. [FBrf0053878] Oliver et al., 1990, Genetics 125: 535--550 Genetic evidence that the ovo locus is involved in Drosophila germ line sex determination. [FBrf0051953] Oliver et al., 1987, Genes Dev. 1: 913--923 The ovo locus is required for sex-specific germ line maintenance in Drosophila. [FBrf0046291]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\Stalker2{}ovoD1rv23 Insertion

Dmel\Stalker2{}ovo^D1rv23 Insertion