Dmel\P{PZ}aret⁰¹²⁸⁴ Insertion

General Information
Symbol	Dmel\P{PZ}aret⁰¹²⁸⁴	Species	D. melanogaster
Name		FlyBase ID	FBti0004784
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	aret, CG31759, Ecol\lacZ	Viability / fertility
Causes allele(s)	aret⁰¹²⁸⁴, CG31759⁰¹²⁸⁴, Ecol\lacZ^aret-01284	Stock availability	1 publicly available
LINE ID	l(2)01284 ms(2)01284
Genomic Location
Chromosomal location	2L ( 33D3 )	Sequence location	2L:12,207,752..12,207,752 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:12,207,752..12,207,752 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	33D3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	33C1-33D5 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026400 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	aret (BDGP Project Members, 1994-1999) CG31759 (BDGP Project Members, 1994-1999, Spradling et al., 1999) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	aret⁰¹²⁸⁴ (BDGP Project Members, 1994-1999, Spradling et al., 1999) CG31759⁰¹²⁸⁴ (BDGP Project Members, 1994-1999, Spradling et al., 1999) Ecol\lacZ^aret-01284 (BDGP Project Members, 1994-1999)

Lethality References partially lethal - majority die \| recessive
Sterility References male sterile \| recessive (Spradling et al., 1999)
Phenotype Manifest In

Detailed Description
Statement Reference A aret[01284] aret[BG01566] mutant background enhances the moderately rough eye phenotype observed upon expression of mbl[C.Scer\UAS] under the control of Scer\GAL4[hs.2sev]. (Vicente-Crespo et al., 2008) Semi-lethal when heterozygous with Df(2L)Prl, and male-sterile phenotype more severe.
Expression Data
Reporter Expression

Additional Information
Statement Reference In wandering third instar larva, no detectable β-gal staining. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)01284 (Gene Disruption Project members, 2001-) ms(2)01284 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11755 P{PZ}aret⁰¹²⁸⁴ CG31759⁰¹²⁸⁴ cn¹/CyO; ry⁵⁰⁶
Linkouts
Comments
	Location 2L:12207751-12207752 determined by FlyBase alignment of dbGSS accession AQ026400 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	l(2)01284 (FlyBase, 1992-) P1755 (Meister and Braun, 1995.10) P{PZ}aret⁰¹²⁸⁴ (FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-, ) P{PZ}ms(2)01284⁰¹²⁸⁴ (FlyBase, 1992-) P{ry^+t7.2=PZ}aret⁰¹²⁸⁴ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000208
References ( 10 )
Research paper	Vicente-Crespo et al., 2008, PLoS ONE 3(2): e1613 Drosophila muscleblind is involved in troponin T alternative splicing and apoptosis. [FBrf0210274] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}aret01284 Insertion

Dmel\P{PZ}aret⁰¹²⁸⁴ Insertion