Dmel\P{hsneo}bel^neo30 Insertion

General Information
Symbol	Dmel\P{hsneo}bel^neo30	Species	D. melanogaster
Name		FlyBase ID	FBti0004786
Feature type	transposable_element_insertion_site
Description
Inserted element	P{hsneo}	Expression data
Affected gene(s)	bel	Viability / fertility
Causes allele(s)	bel^neo30	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 85A5 )	Sequence location
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	85A5 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{hsneo}
Location-dependent role
Size	5.075Kb
Associated alleles	Tn\neoR^Hsp70Bb.PS
Molecular map
Affected Gene(s)
Insertion may affect gene	bel (Berg and Spradling, 1991)
Alleles and Phenotypes
Causes alleles	bel^neo30 (Berg and Spradling, 1991, Johnstone et al., 2005)

Lethality References
Sterility References female fertile (Johnstone et al., 2005) female sterile (Johnstone et al., 2005) male sterile (Berg and Spradling, 1991, Johnstone et al., 2005)
Phenotype Manifest In
	dorsal appendage (Johnstone et al., 2005) onion stage spermatid & nucleus (Johnstone et al., 2005) spermatid
Detailed Description
Statement Reference Homozygous mutant males have testes that have a high frequency of defective onion stage early spermatid cysts: nucleus size often varies and abnormally large mitochondrial derivatives associated with several nuclei are common. Flagella elongate in mutants, nut spermatid bundles are disorganised. Spermatid nuclei also elongate but are scattered instead of clustered at the end of the flagella bundles. In bel^neo30/bel⁶ females oogenesis initially proceeds normally, but at around stage 9 the majority of egg chambers degenerate. A minority of egg chambers proceed beyond this stage to produce mature eggs. bel^neo30/bel⁶ and bel^neo30/bel² ovaries produce eggs that have very fragile chorions and very short dorsal appendages. (Johnstone et al., 2005) Hemizygous males show a more extreme phenotype than homozygotes and are poorly viable if raised at 18^oC. Hemizygous females are sterile and produce eggs with weak chorion. Homozygous females are fertile.
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	10063 w¹¹¹⁸; fs(3)^ mwh¹ P{hsneo}bel^neo30 red¹ e¹/TM3, Sb¹ Ser¹
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{hsneo}bel^neo30 (Berg and Spradling, 1991, FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000050
References ( 3 )
Research paper	Johnstone et al., 2005, Dev. Biol. 277(1): 92--101 Belle is a Drosophila DEAD-box protein required for viability and in the germ line. [FBrf0180115] Berg and Spradling, 1991, Genetics 127: 515--524 Studies on the rate and site-specificity of P-element transposition. [FBrf0054167]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{hsneo}belneo30 Insertion

Dmel\P{hsneo}bel^neo30 Insertion