Dmel\P{ry11}chico¹ Insertion

General Information
Symbol	Dmel\P{ry11}chico¹	Species	D. melanogaster
Name		FlyBase ID	FBti0004803
Feature type	transposable_element_insertion_site
Description
Inserted element	P{ry11}	Expression data
Affected gene(s)	chico	Viability / fertility
Causes allele(s)	chico¹	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2L ( 31B1 )	Sequence location
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	31B1 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{ry11}
Location-dependent role
Size	8.403Kb
Associated alleles	ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	chico (Berg and Spradling, 1991, Parrish et al., 2009)
Alleles and Phenotypes
Causes alleles	chico¹ (Berg and Spradling, 1991, Takeo et al., 2005, Bohni et al., 1999, Wessells et al., 2004, Riesgo-Escovar, 1998.3.10, Parrish et al., 2009)

Lethality References semi-lethal semi-lethal \| recessive (Spradling, 1990.8.1) semi-viable \| recessive (Riesgo-Escovar et al., 1998) viable (Clancy et al., 2001)
Sterility References female sterile (Clancy et al., 2001, Richard et al., 2005) female sterile \| recessive (Spradling, 1990.8.1, Clegg et al., 1993) male semi-sterile \| recessive (Spradling, 1990.8.1)
Phenotype Manifest In
	adult (Junger et al., 2003) adult heart (Wessells et al., 2004) egg chamber (Drummond-Barbosa and Spradling, 2001) eye (Junger et al., 2003, Wittwer et al., 2005) eye \| cell autonomous \| somatic clone (Bohni et al., 1999) follicle cell (Drummond-Barbosa and Spradling, 2001) genital arch \| male \| somatic clone (Shingleton et al., 2005) head capsule \| somatic clone (Bohni et al., 1999) larva (Parrish et al., 2009) maxillary palpus \| male \| somatic clone (Shingleton et al., 2005) nurse cell (Richard et al., 2005) ommatidium (Junger et al., 2003, Wittwer et al., 2005) ovary (Werz et al., 2009, Richard et al., 2005) wing (Junger et al., 2003, Shingleton et al., 2005)
Detailed Description
Statement Reference Heterozygous flies show increased resistance to dry starvation compared to control flies. (Hull-Thompson et al., 2009) Expression of NLaz[Scer\UAS.cHTa] under the control of Scer\GAL4[ppl.PP] can not further increase the starvation resistance of chico[1]/+ flies. (Hull-Thompson et al., 2009) The length of the dorsal hemisegments along the antero-posterior axis in chico[1] mutant larvae is only 10% of that of wild-types. (Parrish et al., 2009) In a fed state, chico[1] flies are anoxia tolerant (heterozygotes are not). (Vigne et al., 2009) chico[1]/chico[flp147E] flies have elevated levels of lipids compared to control flies. (Werz et al., 2009) Dg[323]/chico[1] double heterozygous mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes. (Shcherbata et al., 2007) Heterozygous ovaries transplanted into homozygous females usually show a robust vitellogenic response, with vitellogenic oocytes spanning the entire range of stage 8 to mature stage 14 eggs being seen in the transplanted ovaries. In all cases, the oocytes of the homozygous host female terminate development at the previtellogenic stage 7. Homozygous ovaries transplanted into heterozygous or wild-type females fail to develop oocytes beyond stage 7, even though the host ovaries become fully vitellogenic in each recipient female. Methoprene does not stimulate vitellogenesis or egg production in homozygous females. On day 1 post-eclosion, homozygous females contain an average of 11.2 nurse cells per egg chamber, and on day 4 they have an average of 14.8 nurse cells per egg chamber (a statistically significant difference). (Richard et al., 2005) chico¹ mutants show a reduction in wing size compared to wild-type controls. However, this phenotype is not temperature sensitive as chico¹ flies raised at 25^oC show a similar decrease in wing area compared to chico¹ flies raised at 18^oC to the decrease in wing area observed for control flies at these two temperatures. Males that carry chico¹ Minute clones throughout one side of the genitals and are heterozygous for chico¹ on the other side show smaller genital arches on the homozygous clone side than on the heterozygous side. The difference in size is 16%. Maxillary palps consisting of chico¹ homozygous clones are 45% smaller than paired palps on the same male. (Shingleton et al., 2005) chico¹/+; dally^{sec.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^29BD pupae show the same large pupae and disordered wing patterning phenotype as wild-type animals expressing dally^{sec.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^29BD. (Takeo et al., 2005) chico¹ homozygous adults are abnormally small and have reduced numbers of ommatidia per eye. (Wittwer et al., 2005) The small body size and reduced numbers of ommatidia seen in chico¹ homozygous adults are partially suppressed by B4^susi-1/B4^susi-1. The eye phenotype is also partially suppressed by B4⁰⁵³³⁷/B4⁰⁵³³⁷ (Wittwer et al., 2005) chico¹ homozygous mutants are small, weighing on average 44% of the mass of wild-types. Mean oxygen consumption is not significantly higher than in wild-type flies. Mass-specific resting heat production is the same in chico¹ mutants as wild-type flies. Dietary-restriction of chico¹ mutants has no effect on resting heat production. While the body mass of wild-type flies fed on an energy restricted medium is reduced, chico¹ mutants show no decrease in body mass. (Hulbert et al., 2004) chico¹ flies are small and live ~50% longer than wild-type flies. These flies have low rates of pacing-induced heart failure and arrest, and their heart beat frequency does not increase with age, compared to wild-type flies. chico¹/+ flies have lifespans that are longer than wild-type flies, but shorter than chico¹ homozygotes. Like wild-type flies, these chico¹/+ flies experience stress-induced heart failure and their heart beat frequency decreases with age. There is no difference between male or female flies for any of the heart functions tested in homozygous or heterozygous chico¹ flies. (Wessells et al., 2004) chico¹/chico^flp147E flies show lower body weight, fewer ommatidia, smaller wings, fewer cells per wing and smaller wing cell area than wild type. (Junger et al., 2003) Homozygous chico;foxo double mutants have more, and even slightly smaller, cells than homozygous chico single mutants. (Junger et al., 2003) No obvious defects in photoreceptor axon projections in the medulla are seen in chico¹/+ ; InR^ex15/+ double heterozygous adults. (Song et al., 2003) Photoreceptor cell projection patterns are indistinguishable from wild type in homozygous larvae and in mosaic larvae in which homozygous clones have been induced in the eye. The pattern of R7 and R8 projections in the medulla is similar to wild type in mosaic adults in which homozygous clones have been induced in the eye. Heterozygotes have normal photoreceptor cell projections in the medulla. (Song et al., 2003) The relation between life-span and food concentration is right-shifted in chico¹ females compared to controls. Control and chico¹ females show similar peak life-spans under dietary restriction, but chico¹ females show a peak in mean lifespan at a food concentration 0.8 times that of normal, while control females show a peak in mean lifespan at a food concentration 0.65 times that of normal. (Clancy et al., 2002) chico¹ adults are resistant to starvation conditions compared to wild type. (Oldham et al., 2002) ovo^D1 chico¹ double heterozygotes live as long as chico¹/+ single heterozygotes and significantly longer than ovo^D1/+ single heterozygotes. (Clancy et al., 2001) Homozygous adults are dwarf in size. Homozygous females show an increase of median and maximum life-span of up to 48 and 41% respectively compared to controls. Homozygous males are slightly short-lived. Heterozygous females and males show an increase in median life-span of up to 36 and 13% respectively. Heterozygous females have reduced fecundity and homozygotes are almost sterile. Mutant flies show no resistance to heat stress at 37^oC. Slight resistance to oxidative stress (methyl viologen) is seen in heterozygotes but not in homozygotes. Heterozygotes and homozygotes show some resistance to starvation. (Clancy et al., 2001) chico^+t8 significantly reduces the extended life-span seen in chico¹ heterozygous males and females. (Clancy et al., 2001) The chico¹ mutation partially impaired the ability of follicle cells to proliferate faster in the presence of abundant nutrients. Egg chambers do not develop beyond vitellogenic stages in homozygotes, even in the presence of abundant food. (Drummond-Barbosa and Spradling, 2001) chico¹ flies do not show unexpanded wings, are not dark and do not show male sterility. (Riesgo-Escovar, 2001.2.7) Homozygous clones are not visible in the adult eye because they are out-competed by heterozygous cells. (Wittwer et al., 2001) Homozygosity for chico¹ causes semilethality and an overall delay in development. Flies eclose 2-3 days after their heterozygous siblings. In non-crowded culture conditions, homozygous mutant mothers can produce few viable progeny lacking both maternal and zygotic chico function. Homozygotes have a drastic weight reduction (65% in females, 55% in males) compared to wild-type. Body size reduction is observed at all developmental stages but does not alter the overall proportions of the flies. Homozygous clones in the eye produce morphologically normal ommatidia, except that they are more than 50% smaller than wild-type. Otherwise normal ommatidia with both smaller mutant and larger wild-type photoreceptors are observed at the edges of clones, indicating that chico acts cell autonomously. Autonomy of cell size control is also observed in mutant clones in the wing. Selective removal of chico¹ function in the eye imaginal disc cells generates flies with a strongly reduced head capsule and reduced eyes, whereas the proboscis and the rest of the body are of wild type size. Homozygous mutant clones in the adult eye are rarer, more variable in size and on average smaller than their wild-type sister clones. Clones are more frequently observed in the anterior half of the eye around the equator. In the eye disc, clones are also more variable in size and on average smaller than their wild-type sister clones. TUNEL analysis shows no increase in apoptotic cells in homozygous clones. Clones produced in a Minute background do not reveal enhanced apoptosis compared to wild type clones, or any increase in morphological signs of programmed cell death, in either the imaginal discs or the adult eye. No significant difference is seen in the apoptotic sub-G1 fraction of homozygous mutant cells compared to heterozygous cells. (Bohni et al., 1999) Mutant flies develop slower than sibling controls and have a reduced body size. chico mutant cells differentiate normally but are significantly reduced in size. (Riesgo-Escovar et al., 1998) male fertility poor female-sterile
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	10738 cn¹ P{ry11}chico¹/CyO; ry⁵⁰⁶
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{ry11}chico¹ (FlyBase, 1992-) P{ry11}flp^ry4 (Berg and Spradling, 1991, FlyBase, 1992-) P{ry11}fs(2)4¹ (FlyBase, 1992-) P{ry^+t7.2=ry11}flp^ry4 (FlyBase, 1992-) P{ry^+t7.2=ry11}fs(2)4¹ (FlyBase, 1992-) unnamed (Parrish et al., 2009)
Secondary FlyBase IDs
	FBti0000889 FBti0002798
References ( 26 )

Generate a list of	All references relating to this insertion ( 26 )

List References by type	Research paper ( 21 ) Personal communication to FlyBase ( 3 ) Abstract ( 1 ) FlyBase analysis ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...

Dmel\P{ry11}chico1 Insertion

Dmel\P{ry11}chico¹ Insertion